Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   M5E07_RS02380 Genome accession   NZ_CP098732
Coordinates   483255..484070 (+) Length   271 a.a.
NCBI ID   WP_252221518.1    Uniprot ID   A0AAE9LS73
Organism   Acinetobacter tibetensis strain Y-23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 478255..489070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5E07_RS02350 (M5E07_02350) - 478615..479025 (+) 411 WP_252221510.1 hypothetical protein -
  M5E07_RS02355 (M5E07_02355) - 479178..480461 (+) 1284 WP_252221512.1 glycosyltransferase family 1 protein -
  M5E07_RS02360 (M5E07_02360) - 480490..481041 (+) 552 WP_252221514.1 phosphatase PAP2 family protein -
  M5E07_RS02365 (M5E07_02365) iscX 481122..481322 (+) 201 WP_116760462.1 Fe-S cluster assembly protein IscX -
  M5E07_RS02370 (M5E07_02370) ndk 481449..481880 (+) 432 WP_004983508.1 nucleoside-diphosphate kinase -
  M5E07_RS02375 (M5E07_02375) rlmN 482004..483239 (+) 1236 WP_252221516.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  M5E07_RS02380 (M5E07_02380) pilF 483255..484070 (+) 816 WP_252221518.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M5E07_RS02385 (M5E07_02385) - 484061..484855 (+) 795 WP_116760456.1 helix-turn-helix domain-containing protein -
  M5E07_RS02390 (M5E07_02390) ispG 484876..485991 (+) 1116 WP_252221520.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M5E07_RS02395 (M5E07_02395) hisS 485988..487283 (+) 1296 WP_252221522.1 histidine--tRNA ligase -
  M5E07_RS02400 (M5E07_02400) - 487317..488021 (+) 705 WP_116760450.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 271 a.a.        Molecular weight: 30527.94 Da        Isoelectric Point: 8.8630

>NTDB_id=697465 M5E07_RS02380 WP_252221518.1 483255..484070(+) (pilF) [Acinetobacter tibetensis strain Y-23]
MSKPTYKFACITMFCLSASLVGCITTTTSSAPGFKKDPEKAVEVRTQLAAEYIRKGDLDAAKRALDQALEMEPRDSTANM
MMGVLLQQEGSPANMEKAETHFKRAISADPKNAQARNNYGTYLYVVERYNEAIEQLAIAGSTLGYDQRFKALENLGRIYL
KLGDIANAEKSFKQALQVNPNSYVSMLELSEIFYLRQQIPAATQLYEQFVRLVGQKNQSARALWIGIRIARANGDQMGMQ
VLVNQLRALFPDSQEYTRYLQLQYSTEAVWK

Nucleotide


Download         Length: 816 bp        

>NTDB_id=697465 M5E07_RS02380 WP_252221518.1 483255..484070(+) (pilF) [Acinetobacter tibetensis strain Y-23]
TTGAGTAAACCCACCTACAAATTTGCATGTATAACAATGTTTTGTCTTTCAGCGTCATTAGTGGGTTGTATTACGACAAC
GACTTCGTCAGCACCGGGGTTTAAAAAAGACCCCGAGAAAGCAGTAGAAGTGCGAACTCAATTGGCAGCGGAATATATTC
GTAAAGGCGACCTAGATGCGGCAAAACGCGCGCTCGATCAAGCATTAGAAATGGAACCACGAGATTCCACAGCGAATATG
ATGATGGGCGTGTTGTTACAGCAAGAAGGTAGCCCTGCGAATATGGAAAAAGCGGAGACACATTTTAAACGTGCAATTTC
CGCAGATCCTAAAAATGCGCAAGCTCGTAACAACTATGGTACGTATTTATACGTCGTAGAGCGTTATAATGAAGCGATTG
AACAATTAGCAATTGCTGGTTCAACCTTAGGTTATGATCAGCGATTCAAGGCATTGGAAAATTTAGGACGTATTTACTTA
AAGTTGGGTGATATAGCAAATGCTGAAAAATCATTCAAGCAAGCATTACAAGTGAATCCGAATTCTTATGTTTCAATGTT
AGAGTTGTCAGAAATATTTTATTTAAGACAACAAATTCCTGCTGCGACACAGTTATATGAACAGTTTGTTCGTCTGGTTG
GGCAGAAAAATCAGAGTGCGCGTGCATTATGGATTGGTATTCGTATTGCGCGAGCCAATGGCGATCAGATGGGTATGCAA
GTGTTAGTCAATCAACTGCGTGCATTGTTCCCAGATAGCCAAGAATACACACGTTATTTGCAATTACAGTACAGTACTGA
GGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_1)

(150-181)

Tetratricopeptide repeat (TPR_8)

(43-74)

Tetratricopeptide repeat (TPR_17)

(102-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

73.091

100

0.742
  pilF Acinetobacter baylyi ADP1

68.821

97.048

0.668