Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   NDK36_RS05920 Genome accession   NZ_CP098491
Coordinates   1136972..1140670 (+) Length   1232 a.a.
NCBI ID   WP_014479389.1    Uniprot ID   -
Organism   Bacillus subtilis strain NB205     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1131972..1145670
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDK36_RS05900 (NDK36_05900) - 1132295..1132486 (+) 192 WP_072592521.1 hypothetical protein -
  NDK36_RS05905 (NDK36_05905) cusC 1132420..1132746 (-) 327 WP_033881409.1 four-helix bundle copper-binding protein -
  NDK36_RS05910 (NDK36_05910) yhjR 1132864..1133301 (-) 438 WP_003245806.1 ferritin family protein -
  NDK36_RS05915 (NDK36_05915) addB 1133485..1136985 (+) 3501 WP_014479388.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  NDK36_RS05920 (NDK36_05920) addA 1136972..1140670 (+) 3699 WP_014479389.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  NDK36_RS05925 (NDK36_05925) sbcD 1140742..1141917 (+) 1176 WP_014479390.1 exonuclease subunit SbcD -
  NDK36_RS05930 (NDK36_05930) sbcC 1141914..1145306 (+) 3393 WP_014479391.1 exonuclease subunit SbcC -
  NDK36_RS05935 (NDK36_05935) hlpB 1145320..1145622 (+) 303 WP_003233097.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1232 a.a.        Molecular weight: 141058.39 Da        Isoelectric Point: 5.0963

>NTDB_id=696271 NDK36_RS05920 WP_014479389.1 1136972..1140670(+) (addA) [Bacillus subtilis strain NB205]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIAEA
LEKELVQRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIAMVLNGAKEKLLRA
LELTKAPGGPAPRADNFLDDLAQIDELIQHQDDFSELYKRVPAVSFKRAKAVKGDEFDPALLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLAEMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAENDKGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETETELLLIDNAEDTDASEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKKKTHRNIQYRDIVILLRSMPWAPQIMEELRAHGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLNTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQASASQTDWLLPEFDRYQAKTYLDFIGPALARHRDLGDLAGVPAHADISGHPARFAVQMIHSYDLL
DDDLEERMEEKSERLEAIRRGEPVPGSFAFDEKAREQLSWTYPHQEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPADG
SILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLSHVPSIEEAEQTVHRLYEKELLTEEQKDAIDIEEIVQFFHTEIGGQL
IGAKWKDREIPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYETQ
IQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3699 bp        

>NTDB_id=696271 NDK36_RS05920 WP_014479389.1 1136972..1140670(+) (addA) [Bacillus subtilis strain NB205]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTTTCAACCGGCCAGGATATTCT
TGTGGCAGCGGCTGCGGGCTCTGGTAAAACCGCTGTGCTCGTTGAACGAATGATTCGGAAAATCACCGCGGAGGAAAACC
CAATAGATGTAGACCGTCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAGCACCGAATCGCAGAAGCC
TTGGAAAAAGAGCTTGTACAGCGCCCCGGCTCGCTGCATATCAGACGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
GACGCTTCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTGATCGATCTTGATCCGGGCTTTAGGATAGCTG
ATCAGACGGAAGGCGAGCTGCTCGGTGATGAAGTGCTTGACGAGCTGTTTGAAGACGAATATGCAAAGGGTGAAAAGGCA
TTTTTTGAGCTTGTTGACCGCTATACGACAGACCGGCACGATCTGGATCTGCAATTTCTCGTTAAACAGGTGTACGAGTA
TTCCCGATCCCATCCCAACCCCGAGGCGTGGCTGGAAAGCTTTGTTCATTTGTATGATGTATCAGAAAAGAGCGCCATCG
AGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCG
CTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCGCAGATTGATGAACT
GATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGTCTCTTTTAAGCGTGCCAAAGCAGTAAAAG
GGGATGAGTTCGATCCAGCGCTCCTTGATGAGGCGACAGATTTGAGAAACGGCGCAAAAAAACTGCTTGAAAAGCTCAAA
ACCGACTACTTCACGCGAAGTCCTGAACAGCACTTGAAAAGCCTAGCCGAGATGAAGCCTGTCATTGAAACGCTCGTACA
GCTTGTCATCAGCTATGGAAAACGGTTCGAAGCCGCGAAGCAGGAAAAATCAATCATTGATTTTTCTGATTTGGAGCATT
ACTGTTTAGCGATTTTGACAGCTGAGAATGACAAAGGTGAACGTGAGCCGAGCGAGGCTGCGAGGTTTTATCAGGAACAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTCGTGCAGGAATCGATTCTGCAGCTCGTCACAAGCGGGCC
GGAGGAGACTGGTAACCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCCGAGCCGCTTCTTT
TCCTCTCTAAATACAAACGGTTTACAGAGAGCGGAGAAGGCACGGGGCGGAAAATCGATTTAAATAAAAATTTCCGAAGC
CGGGCTGATATTTTAGACAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTCGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCAGCGTATCCGGACAATGACGAGACGGAAACAGAGCTGCTGCTGATCGACAACGCAG
AAGATACGGATGCAAGCGAGGAAGCAGAAGAGCTTGAAACGGTGCAGTTTGAGGCAAAAGCCATCGCTAAGGAAATTCGC
AAGCTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAAAGAAAACACATCGCAATATTCAGTACCGAGATATCGTGAT
TTTGCTCCGTTCGATGCCGTGGGCTCCGCAAATCATGGAGGAGCTGAGAGCACATGGCATACCGGTTTACGCCAATTTAA
CGTCAGGCTATTTTGAAGCGGTCGAAGTCGCCGTGGCGCTTTCTGTGCTGAAGGTGATTGATAATCCGTATCAGGATATA
CCGCTTGCCTCTGTGCTGCGCTCACCGATTGTCGGAGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATAAAAA
AGCGCCGTACTATGAGGCGATGAAAGACTACCTGGCTGCTGGTGACCGGAACGATGAGCTTTATCAAAAGCTAAATACGT
TTTACGGACATCTGCAAAAATGGCGCGCGTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAC
ACCAAATATATGGATTATGTCGGCGGCATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGTGTTCTTTATGACAGGGC
GCGTCAATATGAATCAACGGCATTTCGCGGCTTGTTCCGTTTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTGTTTGCAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTAAATAAATCGTACCTGCTGGATAAGGA
GCTCGGATTTGGCACGAAGTATATTCATCCGCAATTACGCATCAGCTATCCGACACTTCCGCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCAGAGGAATTGCGTGTTCTCTATGTTGCATTAACGAGAGCGAAGGAAAAGCTGTTTCTG
ATTGGCTCATGTAAGGATCATCAGAAACAGCTTGCAAAATGGCAGGCATCAGCGTCCCAAACTGATTGGCTTCTGCCGGA
ATTTGACCGCTATCAGGCGAAAACGTATCTAGATTTCATCGGCCCGGCTCTTGCCAGGCACAGAGACTTGGGGGATTTGG
CTGGTGTGCCAGCACACGCTGACATCTCAGGCCACCCGGCTCGTTTTGCCGTTCAAATGATCCATTCCTATGATTTGCTT
GATGATGATCTGGAAGAAAGAATGGAAGAAAAAAGCGAGCGCCTAGAAGCGATCCGCCGAGGTGAACCAGTTCCCGGCTC
GTTTGCGTTTGATGAAAAAGCCCGCGAGCAGCTGAGCTGGACCTACCCGCATCAAGAAGTGACGCAAATTCGGACAAAGC
AATCAGTTTCTGAGATCAAAAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCCCCTGTAAAACCGGCTGATGGA
AGCATTCTGTACAGACGTCCCGCTTTTATGATGAAAAAAGGCCTGACAGCGGCAGAGAAAGGGACTGCCATGCATACGGT
GATGCAGCATATCCCGCTGTCACATGTGCCGTCGATAGAAGAAGCTGAGCAGACGGTTCACAGGCTTTATGAAAAAGAGC
TTCTCACTGAAGAACAAAAAGACGCTATTGATATAGAAGAAATCGTGCAATTTTTCCATACGGAAATCGGCGGACAGCTG
ATCGGTGCTAAGTGGAAGGACCGGGAAATACCATTCAGCTTAGCGCTTCCGGCCAAGGAGATCTATCCTGATGCACAGGA
GGCAGATGAGCCGCTTTTAGTGCAGGGTATCATTGACTGTCTCTATGAAACTGAGGACGGATTATATCTATTGGATTATA
AGTCGGACCGGATTGAGGGCAAATTCCAGCATGGATTTGAAGGAGCGGCCCCGATCTTGAAGAAACGATATGAAACGCAA
ATTCAGCTGTACACGAAGGCAGTGGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTTTATTTCTTTGACGGAGG
GCACATTCTGACATTATAG

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1053-1223)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

99.513

100

0.995