Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NDN13_RS17310 Genome accession   NZ_CP098480
Coordinates   3618007..3618813 (+) Length   268 a.a.
NCBI ID   WP_251116334.1    Uniprot ID   -
Organism   Acinetobacter sp. C32I     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3613007..3623813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDN13_RS17285 (NDN13_17280) - 3613936..3615207 (+) 1272 WP_251118261.1 glycosyltransferase family 1 protein -
  NDN13_RS17290 (NDN13_17285) - 3615246..3615797 (+) 552 WP_004802640.1 phosphatase PAP2 family protein -
  NDN13_RS17295 (NDN13_17290) iscX 3615894..3616094 (+) 201 WP_004652532.1 Fe-S cluster assembly protein IscX -
  NDN13_RS17300 (NDN13_17295) ndk 3616202..3616633 (+) 432 WP_004652531.1 nucleoside-diphosphate kinase -
  NDN13_RS17305 (NDN13_17300) rlmN 3616755..3617990 (+) 1236 WP_251116333.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  NDN13_RS17310 (NDN13_17305) pilF 3618007..3618813 (+) 807 WP_251116334.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NDN13_RS17315 (NDN13_17310) - 3618804..3619598 (+) 795 WP_171551121.1 helix-turn-helix domain-containing protein -
  NDN13_RS17320 (NDN13_17315) ispG 3619621..3620736 (+) 1116 WP_004802647.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  NDN13_RS17325 (NDN13_17320) hisS 3620733..3622025 (+) 1293 WP_251116335.1 histidine--tRNA ligase -
  NDN13_RS17330 (NDN13_17325) - 3622055..3622753 (+) 699 WP_167250023.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 268 a.a.        Molecular weight: 30431.40 Da        Isoelectric Point: 9.7100

>NTDB_id=696178 NDN13_RS17310 WP_251116334.1 3618007..3618813(+) (pilF) [Acinetobacter sp. C32I]
MKITRTKTIISTAVGFILLSLTACQSTSTALKKDPEKAVQVRTQLAAEYIRNRDLDAAKRSLDQALKVDSRDANANMMMG
ILLQQEGSASNMEKAEAYFKRAISSEPDNAQARNNYGTYLYQQQRYNEAIEQFTRAGATLGYEQRFQSLENLGRIYLRLG
DAANAEKSFKQALQANRNATISMLELAEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGLRIARANADQLGMQVLV
NQLRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 807 bp        

>NTDB_id=696178 NDN13_RS17310 WP_251116334.1 3618007..3618813(+) (pilF) [Acinetobacter sp. C32I]
TTGAAGATCACTCGAACAAAAACGATTATAAGCACGGCTGTTGGGTTTATTTTATTGAGCTTAACTGCGTGTCAATCGAC
ATCGACAGCATTAAAAAAAGATCCAGAAAAAGCAGTGCAAGTGCGTACTCAACTTGCTGCCGAATATATCCGTAATCGTG
ATCTAGATGCAGCCAAGCGCTCGCTGGACCAAGCCCTAAAAGTCGACTCTCGTGATGCCAATGCCAACATGATGATGGGC
ATCTTGCTGCAACAAGAAGGCAGCGCCAGTAATATGGAAAAGGCAGAAGCTTATTTTAAGCGGGCAATTTCCTCAGAACC
AGACAATGCACAAGCGCGTAATAATTACGGTACATATTTGTATCAACAGCAACGCTATAATGAAGCAATTGAGCAATTTA
CCCGTGCGGGTGCAACTTTAGGCTATGAACAACGGTTTCAGTCGTTGGAAAACCTAGGGCGAATCTATTTAAGATTGGGC
GATGCTGCGAATGCTGAAAAATCATTCAAACAGGCGCTACAAGCGAATCGAAACGCAACCATTTCAATGTTAGAGTTGGC
GGAAATTTTCTATTTGCAACAAAATAATAGAGATGCATCGCAACTATACGAACAATATGTTCGTAATGTAGGACAGAAAA
ACCAAGGTGCTCGAGCACTTTGGATTGGTTTACGCATTGCTCGAGCGAATGCGGATCAATTAGGAATGCAAGTGTTAGTC
AATCAACTGCGTGCACTCTTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATG
GAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(41-70)

Tetratricopeptide repeat (TPR_16)

(89-136)

Tetratricopeptide repeat (TPR_16)

(151-209)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.985

100

0.78
  pilF Acinetobacter baylyi ADP1

67.91

100

0.679