Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NDN12_RS15295 Genome accession   NZ_CP098479
Coordinates   3365723..3366529 (-) Length   268 a.a.
NCBI ID   WP_251110131.1    Uniprot ID   -
Organism   Acinetobacter sp. C26G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3360723..3371529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDN12_RS15275 (NDN12_15270) - 3361783..3362481 (-) 699 WP_167250023.1 tetratricopeptide repeat protein -
  NDN12_RS15280 (NDN12_15275) hisS 3362511..3363803 (-) 1293 WP_167250025.1 histidine--tRNA ligase -
  NDN12_RS15285 (NDN12_15280) ispG 3363800..3364915 (-) 1116 WP_004802647.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  NDN12_RS15290 (NDN12_15285) - 3364938..3365732 (-) 795 WP_167250027.1 helix-turn-helix domain-containing protein -
  NDN12_RS15295 (NDN12_15290) pilF 3365723..3366529 (-) 807 WP_251110131.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NDN12_RS15300 (NDN12_15295) rlmN 3366547..3367782 (-) 1236 WP_251110132.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  NDN12_RS15305 (NDN12_15300) ndk 3367904..3368335 (-) 432 WP_004652531.1 nucleoside-diphosphate kinase -
  NDN12_RS15310 (NDN12_15305) iscX 3368443..3368643 (-) 201 WP_004652532.1 Fe-S cluster assembly protein IscX -
  NDN12_RS15315 (NDN12_15310) - 3368740..3369291 (-) 552 WP_167250033.1 phosphatase PAP2 family protein -
  NDN12_RS15320 (NDN12_15315) - 3369329..3370600 (-) 1272 WP_251111558.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 268 a.a.        Molecular weight: 30481.39 Da        Isoelectric Point: 9.5972

>NTDB_id=696122 NDN12_RS15295 WP_251110131.1 3365723..3366529(-) (pilF) [Acinetobacter sp. C26G]
MNTTRPKTIMAIAVGFTLFGLTACQSTSTTLKKDPEKAVQVRTQLAAEYIRNRDLDSAKRSLDQALKVDSRDANANMMMG
ILLQQEGSASNMEKAEAYFKRAISSEPDNAQARNNYGTYLYQQQRYNEAIEQFTRAGATLGYEQRFQSLENLGRIYLRLG
DVANAEKSFKQALQANRNATISMLELAEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGLRIARANADQLGMQVLV
NQLRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 807 bp        

>NTDB_id=696122 NDN12_RS15295 WP_251110131.1 3365723..3366529(-) (pilF) [Acinetobacter sp. C26G]
TTGAATACCACTCGACCAAAAACGATTATGGCGATAGCTGTTGGGTTTACTTTATTCGGCTTAACAGCATGTCAATCGAC
ATCTACAACATTAAAAAAAGATCCAGAAAAAGCAGTGCAGGTCCGTACCCAACTTGCAGCAGAATATATTCGTAATCGTG
ATTTAGATTCAGCCAAGCGCTCGCTGGATCAAGCCTTAAAAGTTGATTCCCGTGATGCCAATGCCAATATGATGATGGGC
ATCTTGCTACAACAGGAAGGCAGTGCCAGCAATATGGAAAAAGCAGAGGCTTATTTTAAGCGTGCAATTTCCTCAGAACC
AGATAATGCGCAAGCGCGTAATAATTACGGTACATATTTGTATCAACAGCAACGCTATAATGAAGCGATTGAGCAATTTA
CCCGTGCGGGTGCAACCTTAGGTTATGAACAGCGGTTTCAATCGTTGGAAAACCTAGGGCGAATCTATTTGAGATTGGGC
GATGTTGCGAATGCTGAAAAATCGTTCAAACAAGCGCTACAAGCGAATCGAAACGCAACTATTTCAATGTTAGAGTTGGC
GGAAATTTTCTATTTGCAACAAAATAATAGAGATGCATCGCAACTATACGAACAATATGTTCGTAATGTAGGACAGAAAA
ACCAAGGGGCAAGAGCACTTTGGATTGGTCTACGCATTGCTCGAGCGAATGCGGATCAATTAGGAATGCAAGTGTTAGTG
AACCAACTACGTGCGCTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATG
GAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(41-70)

Tetratricopeptide repeat (TPR_16)

(89-136)

Tetratricopeptide repeat (TPR_16)

(151-209)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.731

100

0.787
  pilF Acinetobacter baylyi ADP1

69.084

97.761

0.675