Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   EPG46_RS16430 Genome accession   NZ_AP023219
Coordinates   3418758..3419435 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain M505     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3413758..3424435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPG46_RS16395 gspG 3414238..3414675 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  EPG46_RS16400 gspH 3414683..3415192 (+) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  EPG46_RS16405 gspI 3415189..3415566 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  EPG46_RS16410 gspJ 3415559..3416146 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  EPG46_RS16415 gspK 3416139..3417122 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  EPG46_RS16420 gspL 3417137..3418300 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  EPG46_RS16425 gspM 3418297..3418758 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  EPG46_RS16430 pilD 3418758..3419435 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  EPG46_RS16435 bfr 3419464..3419940 (-) 477 WP_000675504.1 bacterioferritin -
  EPG46_RS16440 bfd 3420012..3420206 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  EPG46_RS16445 chiA 3420375..3423068 (-) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=69578 EPG46_RS16430 WP_000178161.1 3418758..3419435(+) (pilD) [Escherichia coli strain M505]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=69578 EPG46_RS16430 WP_000178161.1 3418758..3419435(+) (pilD) [Escherichia coli strain M505]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment