Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NCL52_RS12910 Genome accession   NZ_CP098417
Coordinates   2517250..2517660 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain N2-10     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2512250..2522660
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NCL52_RS12880 (NCL52_12880) - 2512337..2512477 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  NCL52_RS12885 (NCL52_12885) yqeF 2512783..2513514 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  NCL52_RS12890 (NCL52_12890) cwlH 2513766..2514518 (-) 753 WP_101169548.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  NCL52_RS12895 (NCL52_12895) yqeD 2514705..2515331 (+) 627 WP_033884461.1 TVP38/TMEM64 family protein -
  NCL52_RS12900 (NCL52_12900) gnd 2515350..2516243 (-) 894 WP_047182906.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  NCL52_RS12905 (NCL52_12905) yqeB 2516495..2517217 (+) 723 WP_134983516.1 DUF308 domain-containing protein -
  NCL52_RS12910 (NCL52_12910) nucA/comI 2517250..2517660 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  NCL52_RS12915 (NCL52_12915) sigK 2517856..2518584 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  NCL52_RS12920 (NCL52_12920) - 2518584..2518699 (+) 116 Protein_2502 hypothetical protein -
  NCL52_RS12925 (NCL52_12925) - 2518686..2518862 (-) 177 Protein_2503 recombinase family protein -
  NCL52_RS12930 (NCL52_12930) - 2518820..2518998 (-) 179 Protein_2504 hypothetical protein -
  NCL52_RS12935 (NCL52_12935) psiE 2519562..2519978 (-) 417 WP_003246154.1 phosphate-starvation-inducible protein PsiE -
  NCL52_RS12940 (NCL52_12940) yrkQ 2520024..2521322 (-) 1299 WP_134983213.1 two-component system sensor histidine kinase YrkQ -
  NCL52_RS12945 (NCL52_12945) yrkP 2521309..2522004 (-) 696 WP_021480140.1 two-component response regulator YrkP -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=695395 NCL52_RS12910 WP_009967785.1 2517250..2517660(-) (nucA/comI) [Bacillus subtilis strain N2-10]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=695395 NCL52_RS12910 WP_009967785.1 2517250..2517660(-) (nucA/comI) [Bacillus subtilis strain N2-10]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCTGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGCTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529