Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   NCL52_RS12660 Genome accession   NZ_CP098417
Coordinates   2473812..2474579 (-) Length   255 a.a.
NCBI ID   WP_015384118.1    Uniprot ID   -
Organism   Bacillus subtilis strain N2-10     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2468812..2479579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NCL52_RS12640 (NCL52_12640) ppsR 2468986..2469798 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  NCL52_RS12645 (NCL52_12645) ccpN 2469824..2470462 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  NCL52_RS12650 (NCL52_12650) glyS 2470595..2472634 (-) 2040 WP_064671021.1 glycine--tRNA ligase subunit beta -
  NCL52_RS12655 (NCL52_12655) glyQ 2472627..2473514 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  NCL52_RS12660 (NCL52_12660) recO 2473812..2474579 (-) 768 WP_015384118.1 DNA repair protein RecO Machinery gene
  NCL52_RS12665 (NCL52_12665) yqzL 2474616..2474759 (-) 144 WP_003230053.1 YqzL family protein -
  NCL52_RS12670 (NCL52_12670) era 2474907..2475812 (-) 906 WP_003230051.1 GTPase Era -
  NCL52_RS12675 (NCL52_12675) - 2475793..2476203 (-) 411 WP_032722130.1 cytidine deaminase -
  NCL52_RS12680 (NCL52_12680) unpK 2476322..2476693 (-) 372 WP_015384119.1 diacylglycerol kinase family protein -
  NCL52_RS12685 (NCL52_12685) ybeY 2476674..2477147 (-) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  NCL52_RS12690 (NCL52_12690) pgpH 2477148..2479283 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29332.01 Da        Isoelectric Point: 8.3080

>NTDB_id=695391 NCL52_RS12660 WP_015384118.1 2473812..2474579(-) (recO) [Bacillus subtilis strain N2-10]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKSNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=695391 NCL52_RS12660 WP_015384118.1 2473812..2474579(-) (recO) [Bacillus subtilis strain N2-10]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGTC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTGAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTTTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTCTACTATTTTGATCTGTCGAGACTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAGCATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.216

100

0.992