Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MU859_RS00235 Genome accession   NZ_CP098403
Coordinates   30882..32258 (+) Length   458 a.a.
NCBI ID   WP_188891845.1    Uniprot ID   -
Organism   Lactobacillus kefiranofaciens subsp. kefirgranum strain HL1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 25882..37258
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MU859_RS00210 (MU859_00210) - 26893..27276 (-) 384 Protein_41 hypothetical protein -
  MU859_RS00215 (MU859_00215) - 27483..28325 (-) 843 WP_013853539.1 RhaT/GlcU family sugar-proton symporter -
  MU859_RS00220 (MU859_00220) pepC 28469..29818 (-) 1350 WP_250825443.1 aminopeptidase C -
  MU859_RS00225 (MU859_00225) - 29882..30196 (-) 315 WP_013853541.1 hypothetical protein -
  MU859_RS00230 (MU859_00230) - 30331..30882 (+) 552 WP_250825444.1 dUTP diphosphatase -
  MU859_RS00235 (MU859_00235) radA 30882..32258 (+) 1377 WP_188891845.1 DNA repair protein RadA Machinery gene
  MU859_RS00240 (MU859_00240) gltX 32335..33834 (+) 1500 WP_013853544.1 glutamate--tRNA ligase -
  MU859_RS00245 (MU859_00245) cysS 33930..35363 (+) 1434 WP_013853545.1 cysteine--tRNA ligase -
  MU859_RS00250 (MU859_00250) - 35356..35799 (+) 444 WP_013853546.1 ribonuclease III domain-containing protein -
  MU859_RS00255 (MU859_00255) rlmB 35786..36538 (+) 753 WP_188891849.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  MU859_RS00260 (MU859_00260) - 36679..37227 (+) 549 WP_250825445.1 sigma-70 family RNA polymerase sigma factor -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50171.76 Da        Isoelectric Point: 7.0110

>NTDB_id=695280 MU859_RS00235 WP_188891845.1 30882..32258(+) (radA) [Lactobacillus kefiranofaciens subsp. kefirgranum strain HL1]
MARVKTQYKCHSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSTKATASRLIKKTGINEPVKLDKVKAEKEDRIHTKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSDLSEKYKVLYVSGEESANQIKLRADRLGVGQSNMLLYPETDMHDI
REQINDVKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHSYRILHSVKNRFGAANEIGMFEMENEGLKEVTNPSSIFLDQRLPNSTGSAVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGISFNKASLLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAITMAVASSYTDKEISPIDCFVGEVGL
TGEVRRVDKIEARVKEAAKVGFKRVFIPRHNMHASLNSLGIEVIPVSSIPQALKLVFG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=695280 MU859_RS00235 WP_188891845.1 30882..32258(+) (radA) [Lactobacillus kefiranofaciens subsp. kefirgranum strain HL1]
ATGGCACGAGTAAAAACACAATATAAATGTCATTCTTGTGGCTATATTTCCGCCAGCTATTTGGGACGTTGTCCTAATTG
TGGCGCGTGGAATCAGTTTGAAAAAGAAACTGAAGAAGTACAAAAGCGTTCTACAAAAGCGACTGCTAGCCGTTTAATCA
AAAAGACGGGTATCAATGAACCAGTTAAATTAGACAAGGTTAAGGCAGAAAAAGAAGACCGCATCCATACTAAGTCAGAG
GAGTTAAATCGCGTCTTGGGTGGTGGCATCGTCCCAGGTTCGCTTGTTTTAATTGGCGGGGATCCTGGTATTGGCAAATC
GACCTTGATGTTGCAGATCATGAGCGACTTATCGGAAAAATATAAGGTGCTCTACGTTTCTGGGGAAGAATCAGCTAATC
AGATTAAATTAAGAGCCGATCGTTTAGGCGTGGGTCAAAGTAATATGTTGCTATATCCTGAAACAGACATGCATGATATT
CGCGAACAAATCAACGATGTAAAGCCTGATTTCGTAGTCATCGATTCGATTCAAACCATGAATGAGCCTAGTCTTGATTC
AATGACTGGTTCAGCTTCGCAAGTGCGTGAAGTGACCAGCGAACTCATGAAGATCGCTAAGATGGACGCCATTACCGTGT
TTGTCATTGGTCACGTGACTAAGGAAGGCGCGATTGCAGGACCGAAGATTTTGGAGCACATGGTGGATACTGTCCTCTAT
TTTGAAGGGGATGAACACCATTCTTACCGTATTTTGCACTCTGTGAAAAATCGTTTTGGCGCCGCAAACGAAATCGGCAT
GTTTGAGATGGAAAATGAAGGTTTAAAAGAAGTAACTAATCCATCATCGATTTTTCTTGATCAAAGACTGCCAAACTCTA
CTGGTTCCGCTGTAGTAGTTTCGCTGGAAGGCACTAGGCCACTGCTTGCGGAAATTCAAGCATTGGTTACGCCAACCGCT
TTTGGCTATGCTAAAAGAACGACTTCAGGTATTAGTTTCAATAAGGCTTCATTATTATTGGCCGTGCTTGAAAAACGAGG
CAATTTAATGTTGCAGAACCAAGATGTTTATTTGACTGCGACTGGCGGCATTAAATTAAATGAACCGGCGATTGATTTGG
CTATTACAATGGCAGTAGCTTCAAGCTACACTGATAAAGAAATTTCGCCTATAGATTGTTTTGTAGGTGAAGTAGGGCTA
ACCGGCGAAGTTCGCCGCGTTGATAAAATTGAAGCACGAGTAAAAGAAGCCGCTAAAGTGGGCTTCAAGCGTGTTTTTAT
TCCGCGGCATAATATGCATGCTAGTTTAAACAGCTTAGGCATTGAGGTTATTCCGGTTTCCAGCATTCCACAGGCGTTAA
AATTAGTTTTTGGTTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-223)

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

57.549

99.782

0.574
  radA Streptococcus mitis NCTC 12261

57.549

99.782

0.574
  radA Streptococcus pneumoniae Rx1

57.549

99.782

0.574
  radA Streptococcus pneumoniae D39

57.549

99.782

0.574
  radA Streptococcus pneumoniae R6

57.549

99.782

0.574
  radA Streptococcus pneumoniae TIGR4

57.549

99.782

0.574
  radA Bacillus subtilis subsp. subtilis str. 168

55.338

100

0.555