Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NBN09_RS10975 Genome accession   NZ_CP098396
Coordinates   2360666..2362171 (-) Length   501 a.a.
NCBI ID   WP_089484601.1    Uniprot ID   -
Organism   Burkholderia lata strain JAB02     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2355666..2367171
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBN09_RS10950 (NBN09_10915) - 2356694..2357329 (-) 636 WP_089446443.1 LON peptidase substrate-binding domain-containing protein -
  NBN09_RS10955 (NBN09_10920) rapZ 2357357..2358265 (-) 909 WP_412525281.1 RNase adapter RapZ -
  NBN09_RS10960 (NBN09_10925) hprK 2358341..2359309 (-) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  NBN09_RS10965 (NBN09_10930) - 2359439..2359951 (-) 513 WP_027806888.1 PTS sugar transporter subunit IIA -
  NBN09_RS10970 (NBN09_10935) hpf 2360147..2360506 (-) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  NBN09_RS10975 (NBN09_10940) rpoN/rpoN1 2360666..2362171 (-) 1506 WP_089484601.1 RNA polymerase factor sigma-54 Machinery gene
  NBN09_RS10980 (NBN09_10945) lptB 2362331..2363107 (-) 777 WP_011353224.1 LPS export ABC transporter ATP-binding protein -
  NBN09_RS10985 (NBN09_10950) lptA 2363104..2363769 (-) 666 WP_069248887.1 lipopolysaccharide transport periplasmic protein LptA -
  NBN09_RS10990 (NBN09_10955) lptC 2363790..2364404 (-) 615 WP_412525282.1 LPS export ABC transporter periplasmic protein LptC -
  NBN09_RS10995 (NBN09_10960) - 2364408..2364944 (-) 537 WP_089426347.1 KdsC family phosphatase -
  NBN09_RS11000 (NBN09_10965) kdsD 2364944..2365927 (-) 984 WP_039345999.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54790.92 Da        Isoelectric Point: 4.8740

>NTDB_id=695255 NBN09_RS10975 WP_089484601.1 2360666..2362171(-) (rpoN/rpoN1) [Burkholderia lata strain JAB02]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
TNGSSSSSDRAERDEPAGADEYDGYASGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINSLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=695255 NBN09_RS10975 WP_089484601.1 2360666..2362171(-) (rpoN/rpoN1) [Burkholderia lata strain JAB02]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACACCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCACAGAACCCGCTGCTCGAGAACGACGATGAATGGA
TCGCGAGCCCGCTGCGTGTCGCTGCGGACGGATCGTTGATCGCGCAGACGCCTCCCGCACAGAACACCGAGCCCGCTGCG
ACAAACGGCAGCAGTTCGTCGAGCGATCGTGCCGAGCGCGACGAGCCGGCCGGCGCGGACGAATACGACGGTTATGCATC
CGGCGACGGCAACGACGGCCCGCAGTGGAACCTCGACGAATACGGCCGCTCGAGCGGCGCATCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGCGACCATCTGTCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGATGAAGTGCT
CGCCGACCTGCCGCCCGAGTTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCACTGCTGCACAGCTTCGATCCGG
CCGGGGTCGGTGCCCGTTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACACGCACGCTG
GCACTCGAGATTGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACACGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAAG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCATGCCG
CGCCTGCGGATCAACAGCCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTCAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCCATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGAGGCGCCGCTTCGTCGACAGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.379

100

0.691