Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NBT14_RS07245 Genome accession   NZ_CP098365
Coordinates   1472553..1473926 (-) Length   457 a.a.
NCBI ID   WP_040760502.1    Uniprot ID   A0A5M9ELU2
Organism   Weissella paramesenteroides strain LCW-28     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1467553..1478926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBT14_RS07235 (NBT14_07215) gltX 1469806..1471302 (-) 1497 WP_131473300.1 glutamate--tRNA ligase -
  NBT14_RS07240 (NBT14_07220) - 1471425..1472534 (-) 1110 WP_150191426.1 PIN/TRAM domain-containing protein -
  NBT14_RS07245 (NBT14_07225) radA 1472553..1473926 (-) 1374 WP_040760502.1 DNA repair protein RadA Machinery gene
  NBT14_RS07250 (NBT14_07230) - 1473937..1474479 (-) 543 WP_002827833.1 dUTP diphosphatase -
  NBT14_RS07255 (NBT14_07235) - 1474578..1474856 (-) 279 WP_002827834.1 GNAT family N-acetyltransferase -
  NBT14_RS07260 (NBT14_07240) - 1474911..1475453 (-) 543 WP_002827835.1 NlpC/P60 family protein -
  NBT14_RS07265 (NBT14_07245) - 1475519..1476907 (-) 1389 WP_277362006.1 LysM peptidoglycan-binding domain-containing protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49655.84 Da        Isoelectric Point: 5.1406

>NTDB_id=695224 NBT14_RS07245 WP_040760502.1 1472553..1473926(-) (radA) [Weissella paramesenteroides strain LCW-28]
MAKTKTQFVCSNCGFVSQRYMGRCANCGAWGTFVEEVMQPEKVDRKSRINLAGQVAKVERLNEINIEETPRIKTDNEEFN
RVLGGGIVPGSMVLIGGDPGIGKSTLLLQISGQLANMGNKVLYIAGEESASQIKLRAERLGVRGDSDFLLFPETDMKQIR
QAIDEAKPEFVVIDSIQTMQEPDISSSVGSVAQIRETTAELLQIAKTNNITIFIVGHVTKEGAIAGPKILEHMVDTVLYF
EGDNQRSFRLLRAVKNRFGSTNELGVFDMQHGGLVEVSNPSDLFLEERLADASGSAVVVAMEGTRPILVEVQALVTPTVF
GNAQRTASGIDRNRVSLLMAVLEKRANLLLQNQDAYVRAAGGVKLDEPAIDLAVAIAIASSYNDVETRSTDAFIGEIGLT
GEVRRVPNVVERVNEAKKLGFKRVFVPQHVSEQLAGIDGIQIMPTKTLSEALQNALG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=695224 NBT14_RS07245 WP_040760502.1 1472553..1473926(-) (radA) [Weissella paramesenteroides strain LCW-28]
ATGGCAAAAACAAAAACCCAATTCGTTTGTTCAAATTGTGGTTTTGTCTCACAGCGATACATGGGACGATGTGCTAATTG
TGGTGCATGGGGAACGTTTGTCGAAGAGGTTATGCAACCGGAAAAGGTGGATCGTAAGTCACGAATCAATCTAGCTGGCC
AAGTAGCTAAGGTTGAACGATTGAATGAAATCAACATTGAAGAAACACCACGTATTAAGACAGATAATGAGGAATTTAAC
CGCGTGCTTGGTGGTGGCATTGTCCCCGGTTCCATGGTACTGATTGGTGGTGATCCTGGAATTGGAAAATCAACATTGTT
ACTACAGATTTCTGGTCAGCTAGCTAATATGGGAAATAAAGTCCTATATATTGCAGGTGAAGAGAGTGCGTCACAAATTA
AATTACGTGCAGAACGATTAGGTGTTCGTGGCGATTCAGACTTTTTACTGTTCCCAGAGACAGACATGAAGCAAATCAGA
CAGGCAATTGATGAAGCCAAGCCAGAATTTGTTGTCATTGATTCGATACAAACAATGCAAGAGCCAGATATCAGTTCATC
AGTTGGCTCAGTTGCCCAAATCCGTGAGACGACTGCAGAGTTATTGCAAATTGCAAAGACCAATAATATTACAATTTTTA
TTGTTGGTCATGTTACCAAAGAAGGTGCGATTGCCGGGCCAAAAATTTTAGAGCATATGGTCGATACTGTTCTATATTTT
GAGGGTGACAATCAGCGAAGTTTTAGATTACTACGTGCCGTTAAAAATCGATTTGGATCAACCAATGAGTTAGGTGTCTT
TGATATGCAACATGGCGGTTTAGTTGAAGTATCGAACCCATCAGATCTCTTTCTAGAAGAACGCCTGGCAGATGCGAGTG
GCTCAGCAGTCGTTGTGGCCATGGAAGGCACACGACCAATTTTAGTTGAGGTCCAAGCATTGGTGACGCCAACCGTATTC
GGCAATGCGCAACGGACAGCTTCAGGAATTGACCGAAATCGTGTATCATTGTTAATGGCAGTCTTAGAAAAGCGAGCTAA
CTTGTTGCTACAGAATCAAGATGCCTATGTACGAGCTGCCGGTGGTGTTAAACTTGATGAACCAGCAATTGACTTAGCTG
TCGCAATTGCGATTGCTTCAAGCTACAATGATGTTGAGACACGATCAACAGATGCGTTTATTGGTGAGATTGGACTAACT
GGTGAAGTTAGACGCGTACCTAATGTTGTTGAACGTGTAAATGAGGCAAAAAAGCTGGGATTTAAACGAGTTTTTGTTCC
ACAACATGTGTCAGAACAATTAGCAGGAATTGATGGCATTCAAATTATGCCAACTAAGACTTTGTCAGAGGCATTACAAA
ACGCATTGGGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-428)

AAA domain (AAA_25)

(71-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5M9ELU2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

62.527

100

0.628
  radA Streptococcus pneumoniae Rx1

62.281

99.781

0.621
  radA Streptococcus mitis NCTC 12261

62.281

99.781

0.621
  radA Streptococcus pneumoniae R6

62.281

99.781

0.621
  radA Streptococcus pneumoniae TIGR4

62.281

99.781

0.621
  radA Streptococcus pneumoniae D39

62.281

99.781

0.621
  radA Bacillus subtilis subsp. subtilis str. 168

60.044

100

0.602