Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NAF01_RS18475 Genome accession   NZ_CP098323
Coordinates   3644161..3645231 (-) Length   356 a.a.
NCBI ID   WP_226618334.1    Uniprot ID   -
Organism   Cytobacillus firmus strain PheN7     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3639161..3650231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAF01_RS18430 (NAF01_18430) - 3639655..3640449 (+) 795 WP_250800952.1 YqhG family protein -
  NAF01_RS18435 (NAF01_18435) - 3640493..3640678 (-) 186 WP_035329975.1 YqzE family protein -
  NAF01_RS18440 (NAF01_18440) - 3640757..3641254 (-) 498 WP_226618338.1 shikimate kinase -
  NAF01_RS18445 (NAF01_18445) comGG 3641267..3641650 (-) 384 WP_226618337.1 competence type IV pilus minor pilin ComGG -
  NAF01_RS18450 (NAF01_18450) comGF 3641640..3642119 (-) 480 WP_350457420.1 competence type IV pilus minor pilin ComGF -
  NAF01_RS18455 (NAF01_18455) - 3642070..3642399 (-) 330 WP_226618336.1 phosphatase -
  NAF01_RS18460 (NAF01_18460) comGD 3642383..3642823 (-) 441 WP_197247510.1 competence type IV pilus minor pilin ComGD -
  NAF01_RS18465 (NAF01_18465) comGC 3642820..3643143 (-) 324 WP_197247508.1 competence type IV pilus major pilin ComGC -
  NAF01_RS18470 (NAF01_18470) comGB 3643143..3644174 (-) 1032 WP_226618335.1 competence type IV pilus assembly protein ComGB -
  NAF01_RS18475 (NAF01_18475) comGA 3644161..3645231 (-) 1071 WP_226618334.1 competence type IV pilus ATPase ComGA Machinery gene
  NAF01_RS18485 (NAF01_18485) - 3645500..3645853 (-) 354 WP_226618333.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NAF01_RS18490 (NAF01_18490) - 3646100..3646798 (+) 699 WP_226618332.1 helix-turn-helix domain-containing protein -
  NAF01_RS18495 (NAF01_18495) - 3646952..3647197 (+) 246 WP_009332938.1 DUF2626 domain-containing protein -
  NAF01_RS18500 (NAF01_18500) - 3647232..3648302 (-) 1071 WP_197247803.1 SAM-dependent methyltransferase -
  NAF01_RS18505 (NAF01_18505) - 3648507..3649142 (-) 636 WP_197247501.1 MBL fold metallo-hydrolase -
  NAF01_RS18510 (NAF01_18510) - 3649230..3649709 (+) 480 WP_197247500.1 hypothetical protein -
  NAF01_RS18515 (NAF01_18515) - 3649835..3650008 (+) 174 WP_048008269.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40167.32 Da        Isoelectric Point: 8.3349

>NTDB_id=694913 NAF01_RS18475 WP_226618334.1 3644161..3645231(-) (comGA) [Cytobacillus firmus strain PheN7]
MSIIERLAERIIRDAVRSHASDIHIIPRRKDTLIQLRFGSQLTPRLYLPKEECDRLISHYKFTASMDIGEKRRPQSGAYS
LEVDGQMIGLRFSTLPSSHSESLVIRILPQQEQIPFYQLSLFPDMTRKMLALLKHAHGLIIFTGPTGSGKTTTLYSLLNE
TSHMFHRNVITLEDPIEKENDLVLQVQVNEKAGVSYSAGLKAILRHDPDIIMVGEIRDAETAKIAVRAALTGHLVLSTMH
TRDAEGAVYRLAEFGVNMLEIEQTLVAVTAQRLVELNCPYCEKECSPFCYGYGRWKRASVFELLAGRALNASIKKARGES
SHVKYRTLKEVITKGIALGYIKDTEYSRWVHDDGEA

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=694913 NAF01_RS18475 WP_226618334.1 3644161..3645231(-) (comGA) [Cytobacillus firmus strain PheN7]
TTGAGTATAATTGAAAGATTGGCAGAACGAATTATTAGAGACGCGGTCAGAAGCCATGCTTCCGACATCCACATCATTCC
CCGAAGAAAAGACACACTCATTCAGCTGAGGTTCGGCAGCCAATTGACCCCAAGGCTTTATCTTCCCAAAGAAGAGTGTG
ACAGATTGATTTCCCATTATAAATTTACAGCATCGATGGATATTGGTGAAAAAAGAAGGCCGCAGAGCGGTGCATACTCA
CTTGAAGTAGACGGACAAATGATTGGCCTGCGCTTTTCAACCCTCCCATCAAGCCACAGCGAAAGCCTCGTTATCAGAAT
CCTTCCCCAGCAGGAACAAATTCCTTTTTACCAGCTCAGCCTTTTTCCAGACATGACAAGAAAAATGCTGGCACTGCTGA
AGCATGCTCATGGTTTGATTATTTTCACTGGCCCGACCGGCAGCGGTAAAACCACAACTTTGTATTCCCTTCTGAATGAA
ACCTCCCATATGTTCCATCGCAATGTCATCACCCTGGAAGATCCCATTGAAAAAGAGAATGACTTAGTTCTTCAAGTCCA
AGTGAATGAGAAAGCGGGCGTGTCTTATTCTGCAGGCTTAAAAGCAATCCTTCGCCATGACCCCGATATCATTATGGTCG
GTGAAATCCGGGATGCGGAAACGGCGAAGATTGCAGTTAGAGCAGCTCTCACCGGTCACCTTGTTTTAAGCACCATGCAT
ACCCGGGATGCCGAGGGGGCTGTTTACCGTTTAGCTGAGTTTGGAGTGAACATGCTGGAAATTGAGCAGACTTTGGTGGC
AGTCACCGCACAGCGCCTCGTCGAATTGAATTGTCCATATTGTGAAAAGGAATGCTCCCCATTCTGCTATGGATATGGCA
GGTGGAAAAGGGCGAGTGTGTTTGAATTGCTCGCAGGAAGGGCGCTCAATGCCTCCATAAAGAAGGCAAGAGGGGAAAGC
AGCCATGTTAAGTATCGAACACTGAAGGAAGTCATAACTAAAGGGATTGCTCTAGGCTATATAAAGGATACAGAGTACAG
CAGGTGGGTGCATGACGATGGAGAAGCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.618

100

0.556
  pilB Glaesserella parasuis strain SC1401

39.714

98.315

0.39