Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   PCK13_RS19290 Genome accession   NZ_CP115178
Coordinates   3957671..3959500 (+) Length   609 a.a.
NCBI ID   WP_012767774.1    Uniprot ID   -
Organism   Escherichia coli strain B     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3952671..3964500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCK13_RS19255 (PCK13_19245) - 3953016..3953864 (-) 849 WP_001066390.1 YPO3983 family protein -
  PCK13_RS19260 (PCK13_19250) - 3954047..3954193 (+) 147 Protein_3760 CorA family divalent cation transporter -
  PCK13_RS19265 (PCK13_19255) yigF 3954234..3954614 (-) 381 WP_000032581.1 DUF2628 domain-containing protein -
  PCK13_RS19270 (PCK13_19260) yigG 3954628..3955008 (-) 381 Protein_3762 protein YigG -
  PCK13_RS19275 (PCK13_19265) rarD 3955103..3955993 (-) 891 WP_000339104.1 EamA family transporter RarD -
  PCK13_RS19280 (PCK13_19270) yigI 3956045..3956512 (-) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  PCK13_RS19285 (PCK13_19275) pldA 3956677..3957546 (+) 870 WP_001259695.1 phospholipase A -
  PCK13_RS19290 (PCK13_19280) recQ 3957671..3959500 (+) 1830 WP_012767774.1 ATP-dependent DNA helicase RecQ Machinery gene
  PCK13_RS19295 (PCK13_19285) rhtC 3959564..3960184 (+) 621 WP_000928828.1 threonine export protein RhtC -
  PCK13_RS19300 (PCK13_19290) rhtB 3960246..3960866 (-) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  PCK13_RS19305 (PCK13_19295) pldB 3960977..3961999 (+) 1023 WP_000487654.1 lysophospholipase L2 -
  PCK13_RS19310 (PCK13_19300) yigL 3962007..3962807 (+) 801 WP_000285349.1 sugar/pyridoxal phosphate phosphatase YigL -
  PCK13_RS19315 (PCK13_19305) bioP 3962883..3963782 (+) 900 WP_001196237.1 biotin transporter -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68405.59 Da        Isoelectric Point: 7.2544

>NTDB_id=694271 PCK13_RS19290 WP_012767774.1 3957671..3959500(+) (recQ) [Escherichia coli strain B]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNIRVDVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=694271 PCK13_RS19290 WP_012767774.1 3957671..3959500(+) (recQ) [Escherichia coli strain B]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGGGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAAT
CCCTTTGCTATCAAATCCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCCTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAGCAGCAACTTGAAGT
TATGACAGGCTGTCGCACCGGGCAAATCCGCCTGCTTTATATCGCGCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCG
GAATACGCTGCCCTCGGTCAGCTGCGTCAGCGGTTCCCGACGCTCCCGTTTATGGCGCTGACTGCCACAGCCGATGACAC
CACGCGGCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGATCGCCCGAACATTC
GCTACATGCTGATGGAGAAATTCAAACCGCTCGATCAGTTGATGCGCTACGTGCAGGAACAACGCGGTAAGTCGGGGATT
ATTTACTGCAACAGCCGGGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGAATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATATTCGCGTCGACGTGCAGGAAAAATTTCAGCGCGATGACCTGCAAATTGTGGTGGCGACCG
TGGCGTTTGGCATGGGCATCAACAAGCCTAACGTCCGCTTCGTGGTCCACTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACCGGTCGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCCGATATGGCGTG
GCTACGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAATTGCAGGATATCGAGCGCCATAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTGCTGCTGAACTACTTTGGCGAAGGGCGGCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAATACGATGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGCGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCTGCGCGTCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTATTTAACGAC
GCAACCTTGATTGAGATGGCGGAACAGATGCCGATCACTGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTCGATGGCGACGACGAAGAGTAG

Domains

Predicted by InterProScan.

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

DEAD/DEAH box helicase (DEAD)

(28-187)

HRDC domain (HRDC)

(533-599)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.034

97.209

0.389