Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NBE99_RS08620 Genome accession   NZ_CP098039
Coordinates   1784225..1785316 (-) Length   363 a.a.
NCBI ID   WP_250681686.1    Uniprot ID   -
Organism   Thermosynechococcus sp. HN-54     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1779225..1790316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBE99_RS08605 (NBE99_08605) - 1780523..1782685 (-) 2163 WP_250681683.1 AMIN domain-containing protein -
  NBE99_RS08610 (NBE99_08610) - 1782781..1783515 (-) 735 WP_250681684.1 pilus assembly protein PilO -
  NBE99_RS08615 (NBE99_08615) - 1783512..1784213 (-) 702 WP_250681685.1 PilN domain-containing protein -
  NBE99_RS08620 (NBE99_08620) pilM 1784225..1785316 (-) 1092 WP_250681686.1 type IV pilus biogenesis protein PilM Machinery gene
  NBE99_RS08625 (NBE99_08625) - 1785590..1786144 (-) 555 WP_250681687.1 L,D-transpeptidase -
  NBE99_RS08630 (NBE99_08630) - 1786166..1786510 (-) 345 WP_250681688.1 hypothetical protein -
  NBE99_RS08635 (NBE99_08635) - 1786824..1788053 (-) 1230 WP_250681689.1 acetate/propionate family kinase -
  NBE99_RS08640 (NBE99_08640) comA 1788182..1789879 (+) 1698 WP_250681690.1 phospholipase D-like domain-containing protein Machinery gene

Sequence

Protein


Download         Length: 363 a.a.        Molecular weight: 39699.69 Da        Isoelectric Point: 4.2618

>NTDB_id=693805 NBE99_RS08620 WP_250681686.1 1784225..1785316(-) (pilM) [Thermosynechococcus sp. HN-54]
MGDIVLGNLFAKPKQGLGIELTPERVNIVQLQRQKQGLKITAMASVPLSEGAIEEGRIIDTTAVADAIRQGIEDKRIKQK
EVISAIPMNEAVIRLIRLPAELPDYELREVVLMQEAPLYLPFPREEADVDYQKLGTSLDEDGIERVEILLVGTPREVTDA
YINAFTQAGLQLTALEVTNFALMRTLRDSLQQFVGEAAAIIDIGDEGTEISIVKDGIPQFNRKVPIGKERMQEAISRAMN
LPPSMGVDLIESLTVPLEPMDVTGALNPSGAAILRVLSDLADEIRRSIDFYLNQGESLEVSQLLLAGPGASIGQVDEFFS
QRLNYPTTLVDPISLLGLQTPEEIPLEKRPAWGTVIGLSLRGA

Nucleotide


Download         Length: 1092 bp        

>NTDB_id=693805 NBE99_RS08620 WP_250681686.1 1784225..1785316(-) (pilM) [Thermosynechococcus sp. HN-54]
GTGGGTGATATTGTGCTTGGCAACCTATTTGCAAAACCAAAGCAGGGCTTGGGGATTGAACTCACCCCAGAGCGGGTCAA
CATCGTGCAGCTTCAGCGGCAAAAGCAGGGTCTGAAAATCACAGCGATGGCCTCAGTCCCCTTGAGTGAAGGTGCCATTG
AAGAAGGCCGAATCATTGACACCACTGCCGTTGCCGATGCTATCCGTCAAGGAATTGAGGATAAGCGCATCAAACAAAAA
GAGGTGATCAGTGCCATTCCCATGAATGAAGCGGTGATTCGCCTGATTCGCCTGCCCGCAGAATTGCCTGACTACGAGTT
GCGGGAAGTGGTTCTCATGCAGGAAGCCCCGCTGTACCTTCCTTTTCCCCGTGAAGAGGCTGATGTTGACTATCAAAAGC
TGGGCACCTCCCTTGATGAAGATGGCATTGAGCGGGTGGAGATTCTGCTGGTAGGTACGCCCCGCGAGGTGACCGATGCC
TACATCAATGCCTTTACCCAAGCAGGTTTACAACTGACAGCGCTTGAAGTAACCAATTTTGCCCTCATGCGCACCCTGCG
AGATTCACTGCAACAGTTTGTGGGTGAGGCAGCAGCAATTATTGATATTGGTGATGAGGGCACAGAGATTAGCATTGTCA
AGGATGGTATTCCCCAGTTCAACCGCAAGGTGCCCATTGGCAAAGAACGGATGCAAGAGGCCATCAGCCGTGCCATGAAC
CTGCCTCCCTCCATGGGAGTTGACCTGATCGAGAGTCTCACGGTTCCCCTAGAGCCAATGGATGTCACCGGCGCACTCAA
CCCCAGTGGGGCAGCTATTTTGCGCGTTCTCTCCGATCTTGCGGACGAAATCCGCCGCTCCATTGACTTTTACCTCAACC
AAGGAGAAAGTCTAGAGGTATCCCAGTTACTGCTGGCAGGCCCCGGTGCCAGTATTGGTCAAGTCGATGAGTTTTTTAGT
CAGCGGCTGAACTATCCCACCACATTGGTTGACCCGATTAGTTTGCTGGGACTGCAAACGCCAGAGGAGATTCCCCTCGA
AAAACGTCCTGCTTGGGGCACGGTGATTGGTCTGAGTCTGCGTGGCGCTTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(18-362)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

49.861

99.449

0.496