Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NBE99_RS00740 Genome accession   NZ_CP098039
Coordinates   162577..163968 (-) Length   463 a.a.
NCBI ID   WP_250682618.1    Uniprot ID   -
Organism   Thermosynechococcus sp. HN-54     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 157577..168968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBE99_RS00710 (NBE99_00705) - 158058..158462 (+) 405 WP_250682613.1 DUF3119 family protein -
  NBE99_RS00715 (NBE99_00710) - 158452..159420 (+) 969 WP_250682614.1 DUF3086 domain-containing protein -
  NBE99_RS00720 (NBE99_00715) plsY 159445..160080 (+) 636 WP_250682615.1 glycerol-3-phosphate 1-O-acyltransferase PlsY -
  NBE99_RS00725 (NBE99_00720) argF 160216..161130 (+) 915 WP_250683666.1 ornithine carbamoyltransferase -
  NBE99_RS00730 (NBE99_00725) - 161135..161986 (+) 852 WP_250682616.1 ABC transporter permease -
  NBE99_RS00735 (NBE99_00730) cobU 162002..162568 (+) 567 WP_250682617.1 bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase -
  NBE99_RS00740 (NBE99_00735) radA 162577..163968 (-) 1392 WP_250682618.1 DNA repair protein RadA Machinery gene
  NBE99_RS00745 (NBE99_00740) - 163977..164465 (-) 489 WP_250682619.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  NBE99_RS00750 (NBE99_00745) - 164459..164779 (-) 321 WP_250682620.1 phasin family protein -
  NBE99_RS00755 (NBE99_00750) secG 164889..165122 (+) 234 WP_250682621.1 preprotein translocase subunit SecG -
  NBE99_RS00760 (NBE99_00755) - 165133..165630 (+) 498 WP_250682622.1 GNAT family N-acetyltransferase -
  NBE99_RS00765 (NBE99_00760) - 165614..167008 (-) 1395 WP_250682623.1 serine/threonine-protein kinase -
  NBE99_RS00770 (NBE99_00765) - 167037..167687 (-) 651 WP_250682624.1 hypothetical protein -
  NBE99_RS00775 (NBE99_00770) - 167684..168403 (-) 720 WP_250682625.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 49522.03 Da        Isoelectric Point: 6.9334

>NTDB_id=693793 NBE99_RS00740 WP_250682618.1 162577..163968(-) (radA) [Thermosynechococcus sp. HN-54]
MPKPKVLYICRECGAESLQYFGRCPNCGQWNTLDEQVVTPVETAARRTTPRKSTTPQPLSAKALGEISEQAQSRSSSGFA
ELDRVLGGGIVAGSLVLVAGEPGIGKSTLLLQMAASLGQEWRVLYVCAEESAQQVKLRSQRLGIAAPPSLYLLAETDLDV
ILQELSHLQPHLAILDSIQAVYLPQLTAAPGSVSQVREGTAALMRLAKREQMSLFIVGHVTKEGAIAGPKVLEHLVDTVL
YFEGDRFASHRLVRAVKNRFGASQEIGVFEMGAQGLVEVTNPSALFLGDREPTPGTATIVACEGTRPLVVELQSLVSPTS
YASPRRTATGIEYNRFLQILAVLEKRLGVPLSKLDAYVASSGGLNVAEPAADLGVAVAVVASFRDRWVQPQTVIIGEVGL
GGQVRAVSQLELRLREALKLGFRRAIIPESQACAIAGLDIYPVRRVSDAIVAALAPLTSDESS

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=693793 NBE99_RS00740 WP_250682618.1 162577..163968(-) (radA) [Thermosynechococcus sp. HN-54]
ATGCCCAAGCCTAAAGTACTGTACATCTGCCGCGAGTGTGGTGCCGAGTCTCTGCAATACTTTGGTCGCTGTCCCAATTG
TGGTCAGTGGAATACCCTTGATGAGCAGGTGGTTACCCCAGTAGAGACCGCTGCTCGACGCACAACCCCCCGAAAATCGA
CAACTCCCCAACCCCTCAGTGCCAAAGCTCTAGGAGAGATTAGCGAACAGGCACAAAGTCGTTCTTCCTCTGGATTTGCC
GAGTTGGATCGCGTCTTGGGAGGGGGAATTGTTGCTGGCTCACTGGTGTTGGTGGCAGGGGAACCGGGGATTGGCAAGTC
CACACTGTTGTTGCAAATGGCAGCCTCTTTGGGGCAAGAGTGGCGGGTGCTCTATGTTTGTGCTGAGGAATCGGCGCAAC
AGGTGAAATTGCGATCGCAACGCTTGGGCATTGCTGCCCCCCCTTCCCTTTACCTTTTGGCAGAAACGGATTTAGACGTT
ATTTTGCAGGAACTGAGCCATCTGCAACCCCATCTGGCGATTCTTGATAGCATCCAAGCAGTTTATCTGCCCCAGCTGAC
GGCAGCTCCCGGATCAGTATCTCAGGTGCGCGAGGGAACGGCAGCCTTGATGCGCTTGGCCAAGCGGGAGCAGATGAGTC
TCTTTATTGTGGGTCATGTCACAAAGGAGGGGGCGATCGCTGGCCCCAAGGTGCTGGAACATCTCGTGGATACTGTCCTC
TACTTTGAGGGCGATCGCTTTGCCAGTCACCGCCTCGTACGCGCCGTAAAAAACCGCTTTGGTGCCAGTCAAGAAATTGG
TGTTTTTGAAATGGGAGCGCAGGGCTTAGTAGAAGTAACCAATCCTTCCGCACTGTTTTTGGGCGATCGCGAGCCTACCC
CTGGTACCGCCACCATTGTCGCCTGTGAAGGCACCCGTCCTCTGGTGGTTGAATTGCAAAGTTTGGTCAGTCCCACCAGT
TATGCCTCTCCTCGCCGCACGGCCACGGGCATTGAGTACAACCGCTTTTTGCAAATTTTAGCGGTCTTGGAAAAGCGCTT
AGGGGTTCCCCTCTCAAAGCTGGATGCTTATGTCGCGTCCTCTGGGGGGCTGAATGTGGCGGAACCCGCTGCTGATCTGG
GGGTGGCGGTGGCTGTTGTGGCTAGTTTTCGCGATCGCTGGGTACAACCCCAAACGGTGATTATTGGTGAAGTCGGCTTG
GGGGGTCAGGTGCGGGCAGTCTCCCAGTTGGAGTTACGGCTGCGGGAGGCGCTGAAGTTGGGCTTTCGCCGTGCCATTAT
TCCCGAAAGCCAAGCCTGTGCGATCGCTGGCTTAGATATTTATCCGGTGCGTCGTGTCAGTGATGCCATTGTTGCTGCCC
TTGCACCGCTCACCTCTGATGAATCCTCGTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(75-224)

Subunit ChlI of Mg-chelatase (ChlI)

(370-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.913

99.352

0.486
  radA Streptococcus pneumoniae Rx1

48.741

94.384

0.46
  radA Streptococcus pneumoniae D39

48.741

94.384

0.46
  radA Streptococcus pneumoniae R6

48.741

94.384

0.46
  radA Streptococcus pneumoniae TIGR4

48.741

94.384

0.46
  radA Streptococcus mitis NCTC 12261

48.741

94.384

0.46
  radA Streptococcus mitis SK321

48.741

94.384

0.46