Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   M8561_RS12050 Genome accession   NZ_CP097895
Coordinates   2511432..2512232 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain B1     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 2506432..2517232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8561_RS12020 (M8561_12020) - 2506978..2507643 (+) 666 WP_250353947.1 helix-turn-helix domain-containing protein -
  M8561_RS12025 (M8561_12025) - 2507902..2508039 (+) 138 WP_014470646.1 hypothetical protein -
  M8561_RS12030 (M8561_12030) - 2508133..2509251 (+) 1119 WP_024085589.1 NADH-dependent flavin oxidoreductase -
  M8561_RS12035 (M8561_12035) - 2509377..2509589 (+) 213 WP_024085590.1 spore germination protein -
  M8561_RS12040 (M8561_12040) - 2509715..2509927 (+) 213 WP_014305392.1 spore germination protein -
  M8561_RS12045 (M8561_12045) alr 2510103..2511284 (+) 1182 WP_014305393.1 alanine racemase -
  M8561_RS12050 (M8561_12050) spo0A 2511432..2512232 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  M8561_RS12055 (M8561_12055) spoIVB 2512513..2513793 (-) 1281 WP_007408360.1 SpoIVB peptidase -
  M8561_RS12060 (M8561_12060) recN 2513956..2515686 (-) 1731 WP_024085591.1 DNA repair protein RecN Machinery gene
  M8561_RS12065 (M8561_12065) argR 2515718..2516167 (-) 450 WP_003153173.1 transcriptional regulator ArgR -
  M8561_RS12070 (M8561_12070) - 2516264..2517109 (-) 846 WP_015388017.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=693406 M8561_RS12050 WP_003153177.1 2511432..2512232(-) (spo0A) [Bacillus velezensis strain B1]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=693406 M8561_RS12050 WP_003153177.1 2511432..2512232(-) (spo0A) [Bacillus velezensis strain B1]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCCGATGTGCTCG
TGTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGTGAATCAGAGCTTGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCATCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCTGCGCAAAGCAGCATTATCCGCAGCCAGCCGGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAACCAACCAACTCGGAATTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97