Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M9804_RS01760 Genome accession   NZ_CP097886
Coordinates   336818..338194 (+) Length   458 a.a.
NCBI ID   WP_003631505.1    Uniprot ID   A0AAV4E640
Organism   Lactobacillus helveticus strain BIM B-461 G     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 331818..343194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9804_RS01740 (M9804_01735) - 333332..334189 (+) 858 WP_065866619.1 IS982 family transposase -
  M9804_RS01745 (M9804_01740) - 334427..335686 (-) 1260 WP_172619755.1 RNA-guided endonuclease TnpB family protein -
  M9804_RS01750 (M9804_01745) - 335830..336132 (-) 303 WP_003625857.1 hypothetical protein -
  M9804_RS01755 (M9804_01750) - 336267..336818 (+) 552 WP_003625858.1 dUTP diphosphatase -
  M9804_RS01760 (M9804_01755) radA 336818..338194 (+) 1377 WP_003631505.1 DNA repair protein RadA Machinery gene
  M9804_RS01765 (M9804_01760) gltX 338270..339769 (+) 1500 WP_065866617.1 glutamate--tRNA ligase -
  M9804_RS01770 (M9804_01765) cysS 339862..341292 (+) 1431 WP_020829388.1 cysteine--tRNA ligase -
  M9804_RS01775 (M9804_01770) - 341285..341728 (+) 444 WP_003625863.1 ribonuclease III domain-containing protein -
  M9804_RS01780 (M9804_01775) rlmB 341715..342467 (+) 753 WP_065866616.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  M9804_RS01785 (M9804_01780) - 342610..343155 (+) 546 WP_003631508.1 sigma-70 family RNA polymerase sigma factor -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50293.84 Da        Isoelectric Point: 6.5937

>NTDB_id=693324 M9804_RS01760 WP_003631505.1 336818..338194(+) (radA) [Lactobacillus helveticus strain BIM B-461 G]
MARVKTQYKCRSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSTKATASRLIQKTGLNEPVHLDKIKAEKEERIPTKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLHIMSDLAEKYKVLYVSGEESANQIKLRADRLGVGQSNMLLYPETDMHDI
REQINDVKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHTYRILHSVKNRFGAANEIGMFEMKNEGLREVTNPSSIFLDQRLPNSTGSAVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGISFNKASLLLAVLEKRGNLMLQNQDIYLTATGGIKLNEPAIDLAIAMSVASSYTDKEISPTDCFVGEVGL
TGEVRRVDKIEARVKEAAKVGFKRIFIPKHNMYQSLTENGIEVIPVASIPQALELVFG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=693324 M9804_RS01760 WP_003631505.1 336818..338194(+) (radA) [Lactobacillus helveticus strain BIM B-461 G]
ATGGCTAGAGTAAAAACGCAATATAAATGTCGTTCCTGTGGTTATATTTCAGCTAGTTACTTGGGTCGTTGTCCTAACTG
CGGTGCATGGAATCAATTTGAAAAAGAAACTGAAGAAGTACAGAAAAGATCTACTAAAGCAACGGCAAGCCGTTTAATTC
AAAAAACAGGTCTTAATGAACCTGTTCATTTGGATAAAATCAAGGCTGAAAAAGAAGAACGAATTCCTACTAAGTCCGAA
GAATTAAATCGTGTATTAGGTGGCGGGATCGTTCCTGGATCCCTCGTCTTAATTGGTGGGGACCCTGGTATTGGTAAGTC
AACTTTAATGTTGCATATTATGAGTGATCTGGCAGAAAAATATAAAGTGCTTTATGTTTCAGGAGAAGAGTCAGCTAATC
AAATCAAGCTGAGGGCAGATCGGCTTGGAGTTGGTCAAAGCAACATGCTTTTATATCCAGAAACAGATATGCATGATATT
CGTGAGCAGATCAATGATGTTAAGCCTGATTTTGTCGTTATTGATTCTATTCAAACGATGAATGAACCTAGTCTTGATTC
AATGACTGGTTCTGCTTCACAAGTACGTGAGGTAACAAGTGAATTGATGAAGATTGCTAAGATGGATGCAATTACTGTTT
TTGTTATTGGACACGTGACTAAAGAAGGTGCGATTGCTGGCCCCAAAATTTTGGAACACATGGTGGATACTGTCCTTTAT
TTTGAGGGGGATGAACACCACACGTACCGGATTTTGCATTCCGTAAAAAATCGTTTTGGTGCTGCTAACGAAATCGGCAT
GTTTGAAATGAAAAATGAAGGCTTACGTGAAGTAACCAATCCATCTTCGATTTTCCTTGATCAAAGATTACCTAATTCAA
CTGGGTCAGCCGTAGTAGTTTCACTTGAAGGAACGCGGCCACTTTTGGCAGAAATTCAAGCATTAGTAACGCCAACTGCA
TTTGGCTATGCCAAACGAACTACTTCAGGAATTAGTTTTAATAAAGCTTCACTATTATTAGCGGTTCTTGAAAAACGCGG
CAATTTAATGCTTCAAAATCAGGATATTTATTTAACGGCTACTGGCGGTATTAAATTAAATGAACCTGCAATCGATCTAG
CTATTGCTATGTCAGTAGCATCAAGCTACACCGATAAAGAAATCTCACCGACTGATTGTTTTGTAGGGGAAGTGGGATTA
ACCGGTGAAGTTCGCCGCGTTGATAAAATCGAAGCTAGAGTAAAGGAAGCTGCCAAGGTAGGTTTTAAACGTATTTTTAT
TCCTAAGCATAATATGTATCAAAGTCTAACGGAAAATGGCATTGAAGTAATTCCAGTTGCCAGCATTCCACAGGCATTAG
AATTAGTTTTTGGCTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)

AAA domain (AAA_25)

(66-223)

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

57.112

99.782

0.57
  radA Streptococcus pneumoniae D39

57.112

99.782

0.57
  radA Streptococcus pneumoniae R6

57.112

99.782

0.57
  radA Streptococcus pneumoniae TIGR4

57.112

99.782

0.57
  radA Streptococcus mitis NCTC 12261

57.112

99.782

0.57
  radA Streptococcus mitis SK321

57.112

99.782

0.57
  radA Bacillus subtilis subsp. subtilis str. 168

55.991

100

0.561