Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   MF619_RS17770 Genome accession   NZ_CP097784
Coordinates   3532976..3534121 (+) Length   381 a.a.
NCBI ID   WP_003151067.1    Uniprot ID   A0A6A8LBV3
Organism   Bacillus velezensis strain V 3.14     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3527976..3539121
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MF619_RS17750 (MF619_003536) - 3528451..3529167 (-) 717 WP_014306017.1 Crp/Fnr family transcriptional regulator -
  MF619_RS17755 (MF619_003537) narK 3529265..3530455 (-) 1191 WP_003151073.1 nitrate transporter NarK -
  MF619_RS17760 (MF619_003538) argS 3530589..3532259 (-) 1671 WP_003151071.1 arginine--tRNA ligase -
  MF619_RS17765 (MF619_003539) - 3532256..3532684 (-) 429 WP_003151069.1 DUF1934 domain-containing protein -
  MF619_RS17770 (MF619_003540) rapF 3532976..3534121 (+) 1146 WP_003151067.1 tetratricopeptide repeat protein Regulator
  MF619_RS17775 (MF619_003541) - 3534105..3534224 (+) 120 WP_003151066.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  MF619_RS17780 (MF619_003542) - 3534422..3534625 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  MF619_RS17785 (MF619_003543) - 3534639..3534842 (+) 204 WP_003151062.1 hypothetical protein -
  MF619_RS17795 (MF619_003545) - 3535250..3535531 (+) 282 WP_014306021.1 hypothetical protein -
  MF619_RS17800 (MF619_003546) - 3535575..3536087 (+) 513 WP_032859656.1 stage II sporulation protein M -
  MF619_RS17805 (MF619_003547) - 3536678..3536896 (-) 219 WP_003151056.1 helix-turn-helix transcriptional regulator -
  MF619_RS17810 (MF619_003548) speB 3537066..3537938 (-) 873 WP_003151054.1 agmatinase -
  MF619_RS17815 (MF619_003549) speE 3537998..3538828 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45339.49 Da        Isoelectric Point: 4.9293

>NTDB_id=692401 MF619_RS17770 WP_003151067.1 3532976..3534121(+) (rapF) [Bacillus velezensis strain V 3.14]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEEDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELDQREYLSAIKFFKKAETKLSFIKDTIDKAEYFFKMSEVYYYLKQTYVS
TDYARQAYEIYKAQSSYDIRLIQCQSLFGANFLDLKLYDRAITHFQKAFSLAETEKQPQLMGRTLYNIALCKQSLNDYEA
AITYVEKAIDVFESAKMTPSLPQAYFLMTQIHYKLERYDKALEYHKKGVIYSEESADSVYLHEFEFLKSLYVNGPDEDAM
KRSFDFLESKMMYADIEDFALDAAKYYHKLEDYKKASTYFLKVEEARQHIQGGVTFYEAEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=692401 MF619_RS17770 WP_003151067.1 3532976..3534121(+) (rapF) [Bacillus velezensis strain V 3.14]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAGCTTGCAGGCCTGGAAGAAGATCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGATCAGCGGGAATATTTGTCGGCTATAAAATTTTTTAAAAAGGCTGAGACGAAACTCTCATTTA
TTAAGGATACTATTGATAAAGCCGAGTACTTTTTCAAGATGTCTGAAGTCTACTACTATTTGAAACAAACGTATGTATCT
ACCGATTACGCAAGGCAGGCATATGAAATATATAAAGCTCAGTCGTCATACGATATCAGGCTCATACAATGCCAATCCTT
ATTCGGAGCAAATTTCCTGGATTTAAAACTATATGATCGGGCAATTACTCACTTTCAAAAGGCTTTTTCTTTGGCAGAAA
CAGAAAAACAGCCTCAATTAATGGGCAGGACGCTGTATAACATCGCACTTTGCAAACAAAGCCTGAACGACTATGAAGCA
GCTATAACTTATGTTGAAAAAGCCATTGATGTTTTTGAAAGTGCTAAAATGACCCCTTCTTTGCCCCAAGCATATTTTTT
AATGACACAGATTCATTATAAATTAGAAAGATACGACAAAGCGCTCGAGTATCATAAAAAAGGAGTTATCTATTCCGAAG
AGTCAGCAGATTCAGTTTATTTACACGAATTCGAATTTTTAAAATCTTTGTATGTGAACGGACCTGATGAGGATGCAATG
AAACGGAGTTTTGACTTTTTGGAAAGTAAGATGATGTATGCAGATATAGAAGATTTTGCTCTTGATGCAGCTAAATATTA
TCACAAACTTGAAGATTATAAAAAAGCTTCAACATACTTTTTAAAAGTTGAAGAAGCAAGACAGCATATTCAAGGGGGGG
TAACTTTTTATGAAGCTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(223-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A8LBV3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

74.803

100

0.748
  rapC Bacillus subtilis subsp. subtilis str. 168

53.158

99.738

0.53