Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MF619_RS16860 Genome accession   NZ_CP097784
Coordinates   3353259..3354644 (-) Length   461 a.a.
NCBI ID   WP_025853539.1    Uniprot ID   A0A6A8LC10
Organism   Bacillus velezensis strain V 3.14     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3348259..3359644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MF619_RS16825 (MF619_003352) flgL 3348434..3349351 (-) 918 WP_003151396.1 flagellar hook-associated protein FlgL -
  MF619_RS16830 (MF619_003353) flgK 3349363..3350880 (-) 1518 WP_003151395.1 flagellar hook-associated protein FlgK -
  MF619_RS16835 (MF619_003354) - 3350896..3351378 (-) 483 WP_003151394.1 flagellar protein FlgN -
  MF619_RS16840 (MF619_003355) flgM 3351393..3351659 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  MF619_RS16845 (MF619_003356) - 3351729..3352148 (-) 420 WP_003151392.1 TIGR03826 family flagellar region protein -
  MF619_RS16850 (MF619_003357) comFC 3352222..3352911 (-) 690 WP_196769287.1 phosphoribosyltransferase family protein Machinery gene
  MF619_RS16855 (MF619_003358) - 3352917..3353201 (-) 285 WP_003151389.1 late competence development ComFB family protein -
  MF619_RS16860 (MF619_003359) comFA 3353259..3354644 (-) 1386 WP_025853539.1 DEAD/DEAH box helicase Machinery gene
  MF619_RS16865 (MF619_003360) - 3354752..3355600 (-) 849 WP_032857563.1 DegV family protein -
  MF619_RS16870 (MF619_003361) degU 3355698..3356387 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  MF619_RS16875 (MF619_003362) degS 3356464..3357627 (-) 1164 WP_003151385.1 two-component sensor histidine kinase DegS Regulator
  MF619_RS16880 (MF619_003363) - 3357850..3358497 (+) 648 WP_003151384.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52557.14 Da        Isoelectric Point: 10.1848

>NTDB_id=692395 MF619_RS16860 WP_025853539.1 3353259..3354644(-) (comFA) [Bacillus velezensis strain V 3.14]
MAEGASEFSEVRDFFYGRHLLRSEIPFSDHCIKRFIEKEYITAEPSIIRRKNRYVCQRCGQSDQACFAAFWAPSAKRQIT
YCRACVMMGRADELTLLYSWNQAPENRWKPVKLSWEGTLTDGQKQAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNQGLRVCVATPRTDVVLELLPRLKKAFEKVDVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKNAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQSQLPVFLFVPSVHVLKKTTDYFQKLNIRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVLFHFGITVGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=692395 MF619_RS16860 WP_025853539.1 3353259..3354644(-) (comFA) [Bacillus velezensis strain V 3.14]
ATGGCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
TTCAGATCATTGCATCAAGCGTTTTATAGAAAAAGAATATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCTGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATTATTATATTCTTGGAATCAAGCCCCTGAAAACAG
ATGGAAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCAGGCCGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCTGGTATCGAGTTT
GCCCTGAATCAAGGTTTGCGCGTCTGTGTCGCGACTCCGCGCACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGACGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGCTTGACTCCGCTGATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGGTTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAATGCTGAAGCCGGTTTGCTGAAAAGCGTTCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGCCAAAAACTTCAATCACAGCTGCCGGTTTTCTTATTCGTTCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAGACG
GCCGGCTTGATCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCTAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCCTGTTTCATTTCGGAATTACAGTCGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(118-266)

Helicase conserved C-terminal domain (Helicase_C)

(328-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A8LC10

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

63.261

99.783

0.631