Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   MF619_RS12030 Genome accession   NZ_CP097784
Coordinates   2427306..2428343 (-) Length   345 a.a.
NCBI ID   WP_024085602.1    Uniprot ID   -
Organism   Bacillus velezensis strain V 3.14     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2422306..2433343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MF619_RS11980 (MF619_002389) tasA 2422541..2423326 (-) 786 WP_003153102.1 biofilm matrix protein TasA -
  MF619_RS11985 (MF619_002390) sipW 2423390..2423974 (-) 585 WP_025852917.1 signal peptidase I SipW -
  MF619_RS11990 (MF619_002391) tapA 2423946..2424617 (-) 672 WP_025852919.1 amyloid fiber anchoring/assembly protein TapA -
  MF619_RS11995 (MF619_002392) - 2424876..2425205 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  MF619_RS12000 (MF619_002393) - 2425245..2425424 (-) 180 WP_003153093.1 YqzE family protein -
  MF619_RS12005 (MF619_002394) comGG 2425481..2425858 (-) 378 WP_014305410.1 competence type IV pilus minor pilin ComGG Machinery gene
  MF619_RS12010 (MF619_002395) comGF 2425859..2426359 (-) 501 WP_226565836.1 competence type IV pilus minor pilin ComGF -
  MF619_RS12015 (MF619_002396) comGE 2426268..2426582 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  MF619_RS12020 (MF619_002397) comGD 2426566..2427003 (-) 438 WP_025852922.1 competence type IV pilus minor pilin ComGD Machinery gene
  MF619_RS12025 (MF619_002398) comGC 2426993..2427301 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  MF619_RS12030 (MF619_002399) comGB 2427306..2428343 (-) 1038 WP_024085602.1 competence type IV pilus assembly protein ComGB Machinery gene
  MF619_RS12035 (MF619_002400) comGA 2428330..2429400 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  MF619_RS12040 (MF619_002401) - 2429592..2430542 (-) 951 WP_003153082.1 magnesium transporter CorA family protein -
  MF619_RS12045 (MF619_002402) - 2430688..2431989 (+) 1302 WP_014305416.1 hemolysin family protein -
  MF619_RS12050 (MF619_002403) - 2432027..2432857 (-) 831 WP_032856863.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39618.91 Da        Isoelectric Point: 9.5658

>NTDB_id=692373 MF619_RS12030 WP_024085602.1 2427306..2428343(-) (comGB) [Bacillus velezensis strain V 3.14]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLKLQLHKRQLAELTDGIRRLREGDAFYEVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGELLQRKLMQTNQIKKMLRYPMFLISSVCVMFYILQSLIIPQFSGIYQSMNMNTSGATAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPYYEKDLAAAVSHGQANGLLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=692373 MF619_RS12030 WP_024085602.1 2427306..2428343(-) (comGB) [Bacillus velezensis strain V 3.14]
ATGAAGCGGATTAAACGAACCTGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGTCTGATTGAAGGGCTCAGACTGCTGAAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGACGGACGGAATCC
GCCGGCTGAGGGAAGGTGATGCGTTTTACGAAGTGCTTGAGGCACTGTCTTTTCATAAAGAAGCCGTCTCTATCTGCTAT
TTCGCAGAGAAACACGGAGAATTGCCGGGGGCGATGAAACAAAGCGGCGAATTATTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTCTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACATCCGGTGCGACTGCTTTCATCTTTGCATTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTGTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAATCCCCGCAAGATAAAATGCTCATTGTTGTGAAAATACCCCTCTTGGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
CGGGCATCCGTATTATGAAAAAGATCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCTGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

65.635

93.623

0.614