Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MF619_RS01445 Genome accession   NZ_CP097784
Coordinates   231051..231488 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain V 3.14     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 226051..236488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MF619_RS01405 (MF619_000281) - 226630..226794 (-) 165 WP_370648487.1 molybdopterin dinucleotide binding domain-containing protein -
  MF619_RS01410 (MF619_000282) - 226908..228110 (+) 1203 WP_032857854.1 GTP-binding protein -
  MF619_RS01415 (MF619_000283) - 228175..229311 (+) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  MF619_RS01420 (MF619_000284) - 229326..229760 (+) 435 WP_003156592.1 RDD family protein -
  MF619_RS01425 (MF619_000285) - 229878..230153 (+) 276 WP_369719109.1 YckD family protein -
  MF619_RS01430 (MF619_000286) - 230257..230373 (+) 117 WP_015388718.1 family 1 glycosylhydrolase -
  MF619_RS01435 (MF619_000287) - 230372..230590 (+) 219 Protein_220 family 1 glycosylhydrolase -
  MF619_RS01440 (MF619_000288) nin/comJ 230631..231029 (-) 399 WP_014304317.1 competence protein ComJ Regulator
  MF619_RS01445 (MF619_000289) nucA/comI 231051..231488 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MF619_RS01450 (MF619_000290) hxlB 231847..232404 (-) 558 WP_003156587.1 6-phospho-3-hexuloisomerase -
  MF619_RS01455 (MF619_000291) hxlA 232401..233036 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  MF619_RS01460 (MF619_000292) - 233268..233630 (+) 363 WP_014304320.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=692335 MF619_RS01445 WP_003156588.1 231051..231488(-) (nucA/comI) [Bacillus velezensis strain V 3.14]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=692335 MF619_RS01445 WP_003156588.1 231051..231488(-) (nucA/comI) [Bacillus velezensis strain V 3.14]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641