Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   N6H00_RS22145 Genome accession   NZ_CP114036
Coordinates   5229816..5231240 (-) Length   474 a.a.
NCBI ID   WP_268973560.1    Uniprot ID   -
Organism   Streptomyces sp. S465     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5224816..5236240
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N6H00_RS22125 (N6H00_22125) - 5224843..5225454 (-) 612 WP_268973556.1 SigE family RNA polymerase sigma factor -
  N6H00_RS22130 (N6H00_22130) - 5225745..5226671 (-) 927 WP_268973558.1 A/G-specific adenine glycosylase -
  N6H00_RS22135 (N6H00_22135) - 5226922..5227785 (+) 864 WP_268973559.1 hypothetical protein -
  N6H00_RS22140 (N6H00_22140) disA 5228001..5229059 (-) 1059 WP_268977070.1 DNA integrity scanning diadenylate cyclase DisA Machinery gene
  N6H00_RS22145 (N6H00_22145) radA/sms 5229816..5231240 (-) 1425 WP_268973560.1 DNA repair protein RadA Machinery gene
  N6H00_RS22150 (N6H00_22150) - 5231451..5233145 (+) 1695 WP_268973561.1 sigma-70 family RNA polymerase sigma factor -
  N6H00_RS22155 (N6H00_22155) - 5233209..5234051 (-) 843 WP_268977071.1 hypothetical protein -
  N6H00_RS22160 (N6H00_22160) - 5234210..5235139 (+) 930 WP_268973562.1 Ppx/GppA phosphatase family protein -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 50005.93 Da        Isoelectric Point: 7.4630

>NTDB_id=692160 N6H00_RS22145 WP_268973560.1 5229816..5231240(-) (radA/sms) [Streptomyces sp. S465]
MAARSSSRSSAKDRPSYRCTECGWTTAKWLGRCPECHAWGTVEEYGAPAVRTTTPGRVTSAALPIGQVDGRQATARSTGV
DELDRVLGGGLVPGAVVLLAGEPGVGKSTLLLDVAAKAASDEHRTLYITGEESASQVRLRADRIGALSDHLYLAAETDLS
AVLGHLDSVKPSLLVLDSVQTVASPEIDGAPGGMAQVREVAGALIRASKERGMSTLLVGHVTKDGAIAGPRLLEHLVDVV
LHFEGDRHARLRLVRGVKNRYGATDEVGCFELHDEGITGLADPSGLFLTRRDEPVPGTCLTVTLEGRRPLVAEVQALTVD
SQIPSPRRTTSGLETSRVSMMLAVLEQRGRISALGKRDIYSATVGGVKLSEPAADLAVTLALASAASDTPLPKNLVAIGE
VGLAGEVRRVTGVQRRLAEAARLGFTHALVPGDPGRIPDGMRVLEVADIGDALRVLPRRRERSEKARSAAEDRG

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=692160 N6H00_RS22145 WP_268973560.1 5229816..5231240(-) (radA/sms) [Streptomyces sp. S465]
ATGGCTGCCCGTAGCTCCTCCCGTTCATCCGCCAAGGACCGGCCCTCCTACCGCTGCACGGAGTGCGGCTGGACCACCGC
CAAGTGGCTCGGCCGCTGCCCGGAGTGCCATGCGTGGGGCACGGTCGAGGAGTACGGCGCGCCCGCGGTGCGGACCACCA
CACCCGGCCGGGTCACCTCGGCCGCCCTGCCCATCGGCCAGGTCGACGGCCGTCAGGCCACCGCGCGCAGCACCGGCGTG
GACGAGCTCGACCGGGTGCTCGGCGGCGGGCTGGTCCCGGGCGCGGTCGTGCTGCTCGCCGGGGAGCCCGGGGTCGGCAA
GTCCACGCTGCTGCTGGACGTCGCCGCCAAGGCCGCCTCCGACGAGCACCGCACGCTCTACATCACCGGTGAGGAGTCGG
CGAGCCAGGTGCGGCTGCGCGCGGACCGCATCGGCGCGCTGAGCGATCATCTCTACCTCGCCGCCGAGACCGACCTCTCC
GCGGTCCTCGGCCATCTCGACTCGGTCAAGCCCTCGCTGCTCGTCCTGGACTCGGTGCAGACCGTCGCCTCGCCCGAGAT
CGACGGCGCGCCCGGCGGGATGGCGCAGGTGCGCGAGGTGGCCGGGGCGCTCATCCGCGCCTCCAAGGAGCGCGGTATGT
CCACGCTGCTGGTGGGCCACGTCACCAAGGACGGCGCCATCGCGGGACCCCGGCTGCTGGAGCATCTGGTCGATGTCGTC
CTGCACTTCGAGGGCGACCGGCACGCCCGGCTGCGGCTGGTCCGGGGCGTGAAGAACCGCTACGGGGCGACGGACGAGGT
CGGCTGTTTCGAGCTGCACGACGAGGGCATCACCGGGCTCGCCGACCCCTCCGGGCTGTTCCTCACCCGCCGCGACGAGC
CGGTGCCGGGCACCTGTCTGACGGTCACCCTGGAGGGCCGCCGGCCGCTGGTGGCCGAGGTGCAGGCGCTCACCGTCGAC
TCCCAGATCCCCTCCCCGCGCCGCACCACCTCCGGTCTGGAGACCTCCCGGGTCTCGATGATGCTGGCCGTGCTGGAGCA
GCGCGGCCGGATCAGCGCGCTGGGCAAGCGGGACATCTACAGCGCCACGGTCGGCGGGGTGAAGCTGTCCGAGCCCGCCG
CGGACCTCGCCGTGACGCTCGCGCTGGCCAGCGCCGCCAGTGACACCCCGCTGCCCAAGAACCTGGTGGCCATCGGCGAG
GTGGGGCTCGCGGGCGAGGTCCGGCGGGTCACCGGGGTGCAGCGGCGGCTGGCGGAGGCGGCCCGGCTCGGTTTCACCCA
CGCCCTGGTACCGGGCGACCCGGGCCGGATACCGGACGGGATGCGGGTGCTGGAGGTCGCGGACATCGGGGACGCGCTGC
GGGTGCTGCCCCGGCGGCGGGAGCGGAGTGAGAAGGCGCGGTCGGCCGCGGAGGATCGCGGCTGA

Domains

Predicted by InterProScan.

Subunit ChlI of Mg-chelatase (ChlI)

(372-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(17-44)

AAA domain (AAA_25)

(85-225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

43.929

95.57

0.42
  radA Streptococcus mitis NCTC 12261

42.291

95.781

0.405
  radA Streptococcus pneumoniae D39

42.35

95.148

0.403
  radA Streptococcus pneumoniae TIGR4

42.35

95.148

0.403
  radA Streptococcus pneumoniae R6

42.35

95.148

0.403
  radA Streptococcus pneumoniae Rx1

42.35

95.148

0.403
  radA Streptococcus mitis SK321

43.56

90.084

0.392