Detailed information    

insolico Bioinformatically predicted

Overview


Name   nucA/comI   Type   Machinery gene
Locus tag   MF628_RS26635 Genome accession   NZ_CP097767
Coordinates   5730560..5731009 (-) Length   149 a.a.
NCBI ID   WP_250271684.1    Uniprot ID   -
Organism   Paenibacillus polymyxa strain R 5.31     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5722293..5757633 5730560..5731009 within 0


Gene organization within MGE regions


Location: 5722293..5757633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MF628_RS26590 (MF628_005293) - 5722419..5722643 (-) 225 Protein_5189 hypothetical protein -
  MF628_RS26595 (MF628_005294) - 5722707..5722987 (+) 281 Protein_5190 IS1595 family transposase -
  MF628_RS26600 (MF628_005295) - 5723012..5723408 (-) 397 Protein_5191 VOC family protein -
  MF628_RS26605 (MF628_08980) - 5723842..5724366 (-) 525 WP_250271678.1 hypothetical protein -
  MF628_RS26610 (MF628_005297) - 5724750..5725175 (-) 426 WP_416383368.1 hypothetical protein -
  MF628_RS26615 (MF628_005298) - 5725624..5727043 (-) 1420 Protein_5194 transcriptional regulator -
  MF628_RS26620 (MF628_005299) - 5727202..5727384 (-) 183 WP_250271679.1 hypothetical protein -
  MF628_RS26625 (MF628_005300) - 5727758..5727946 (+) 189 WP_250271681.1 hypothetical protein -
  MF628_RS26630 (MF628_005301) - 5728887..5729957 (-) 1071 WP_250271683.1 hypothetical protein -
  MF628_RS26635 (MF628_005302) nucA/comI 5730560..5731009 (-) 450 WP_250271684.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MF628_RS26640 (MF628_005303) - 5731148..5731456 (-) 309 WP_250271686.1 DUF2500 family protein -
  MF628_RS26645 (MF628_005304) - 5732766..5733671 (-) 906 WP_250271688.1 copper amine oxidase N-terminal domain-containing protein -
  MF628_RS26650 (MF628_005305) - 5733906..5734595 (+) 690 WP_250271690.1 hypothetical protein -
  MF628_RS26655 (MF628_005306) - 5734808..5735017 (-) 210 WP_190297201.1 hypothetical protein -
  MF628_RS26660 (MF628_005307) - 5735111..5735226 (-) 116 Protein_5203 2-pyrone-4,6-dicarboxylate hydrolase -
  MF628_RS26665 (MF628_005308) - 5735266..5736417 (-) 1152 WP_242210790.1 epoxide hydrolase family protein -
  MF628_RS26670 (MF628_08985) - 5736525..5737379 (+) 855 WP_242210789.1 helix-turn-helix domain-containing protein -
  MF628_RS26675 (MF628_005310) - 5737695..5738165 (-) 471 WP_250271692.1 hypothetical protein -
  MF628_RS26680 (MF628_08990) - 5738938..5740368 (+) 1431 WP_250271693.1 radical SAM/SPASM domain-containing protein -
  MF628_RS26685 (MF628_005312) - 5740424..5740570 (-) 147 WP_250271696.1 hypothetical protein -
  MF628_RS27255 - 5740539..5740718 (-) 180 WP_416383397.1 SIS domain-containing protein -
  MF628_RS26690 (MF628_08995) - 5740956..5741243 (-) 288 WP_250271697.1 helix-turn-helix domain-containing protein -
  MF628_RS26695 (MF628_005314) - 5741233..5741415 (-) 183 WP_060628847.1 YvrJ family protein -
  MF628_RS26700 (MF628_005315) - 5741421..5742017 (-) 597 WP_250271700.1 RNA polymerase sigma factor -
  MF628_RS26705 (MF628_005316) - 5742809..5743669 (-) 861 WP_250271702.1 hypothetical protein -
  MF628_RS27260 - 5743926..5744186 (-) 261 Protein_5214 MarR family transcriptional regulator -
  MF628_RS26710 (MF628_005317) - 5744701..5745786 (-) 1086 WP_250271704.1 major royal jelly family protein -
  MF628_RS26715 (MF628_005318) - 5746341..5746622 (-) 282 WP_250271705.1 hypothetical protein -
  MF628_RS26720 (MF628_005319) - 5746813..5747055 (+) 243 WP_171644152.1 spore gernimation protein GerQ -
  MF628_RS26725 (MF628_005320) - 5747068..5747454 (+) 387 WP_044785937.1 spore coat protein -
  MF628_RS26730 (MF628_005321) - 5747451..5748587 (+) 1137 WP_250271708.1 zinc-dependent alcohol dehydrogenase -
  MF628_RS26735 (MF628_005322) - 5748619..5748819 (+) 201 WP_250271710.1 hypothetical protein -
  MF628_RS26740 (MF628_005323) - 5748831..5749127 (+) 297 WP_250271712.1 spore coat protein -
  MF628_RS26745 (MF628_005324) - 5750313..5751899 (+) 1587 WP_250271714.1 spore germination protein -
  MF628_RS26750 (MF628_005325) - 5751914..5753101 (+) 1188 WP_323873283.1 Ger(x)C family spore germination protein -
  MF628_RS26755 (MF628_005326) - 5753098..5753322 (+) 225 WP_250238797.1 hypothetical protein -
  MF628_RS26760 (MF628_005327) - 5753333..5754445 (+) 1113 WP_323873284.1 GerAB/ArcD/ProY family transporter -
  MF628_RS26765 (MF628_005328) - 5754942..5755256 (-) 315 WP_250271715.1 DMT family transporter -
  MF628_RS26770 (MF628_005329) - 5755260..5755601 (-) 342 WP_025723001.1 DMT family transporter -
  MF628_RS26775 (MF628_005330) - 5755877..5756395 (-) 519 WP_250274071.1 GNAT family N-acetyltransferase -
  MF628_RS26780 (MF628_005331) - 5756422..5756874 (-) 453 WP_076293702.1 MarR family winged helix-turn-helix transcriptional regulator -
  MF628_RS26785 (MF628_005332) - 5756989..5757633 (+) 645 WP_250271716.1 YczE/YyaS/YitT family protein -

Sequence


Protein


Download         Length: 149 a.a.        Molecular weight: 16292.54 Da        Isoelectric Point: 6.4783

>NTDB_id=692120 MF628_RS26635 WP_250271684.1 5730560..5731009(-) (nucA/comI) [Paenibacillus polymyxa strain R 5.31]
MIRWMITLLATVLLAGCSVQQQVEVTPSKTAPVASQVTQVSLETVKLEFPSAKYPETAQHIKEAIAAGHTNTCTIDRDGA
DHNRELSLKDVPTRKGKDRDEWPMAMCAEGGVGADVKYISPKDNRGAGSWVGHKLNEYPDGTKIEFIIN

Nucleotide


Download         Length: 450 bp        

>NTDB_id=692120 MF628_RS26635 WP_250271684.1 5730560..5731009(-) (nucA/comI) [Paenibacillus polymyxa strain R 5.31]
ATGATTAGATGGATGATTACCTTACTGGCTACAGTCCTATTAGCTGGCTGCAGTGTGCAACAACAGGTAGAAGTTACACC
GAGCAAAACAGCGCCTGTTGCTTCACAAGTAACCCAAGTATCATTAGAAACCGTAAAACTTGAGTTTCCCTCAGCAAAGT
ACCCGGAAACAGCACAGCACATCAAGGAGGCCATAGCAGCCGGACACACCAATACATGTACCATTGATCGTGACGGAGCT
GATCATAACCGTGAACTGTCACTTAAAGATGTACCCACGCGCAAGGGCAAAGACCGCGATGAATGGCCAATGGCAATGTG
TGCGGAAGGCGGAGTGGGAGCGGACGTTAAATACATATCACCAAAGGATAACCGTGGGGCTGGATCATGGGTAGGTCACA
AGCTGAATGAATATCCAGACGGCACAAAGATTGAATTTATTATTAATTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.569

68.456

0.49