Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MW290_RS31555 Genome accession   NZ_CP097636
Coordinates   3707868..3709406 (+) Length   512 a.a.
NCBI ID   WP_250198281.1    Uniprot ID   A0A1S6R6J6
Organism   Aquincola tertiaricarbonis strain RN12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3702868..3714406
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW290_RS31530 (MW290_31525) argS 3703152..3704870 (+) 1719 WP_250198276.1 arginine--tRNA ligase -
  MW290_RS31535 (MW290_31530) - 3704867..3705541 (+) 675 WP_250198277.1 SPOR domain-containing protein -
  MW290_RS31540 (MW290_31535) - 3705633..3706286 (+) 654 WP_250198278.1 thiol:disulfide interchange protein DsbA/DsbL -
  MW290_RS31545 (MW290_31540) lptA 3706465..3707058 (+) 594 WP_250198279.1 lipopolysaccharide transport periplasmic protein LptA -
  MW290_RS31550 (MW290_31545) lptB 3707064..3707846 (+) 783 WP_250198280.1 LPS export ABC transporter ATP-binding protein -
  MW290_RS31555 (MW290_31550) rpoN/rpoN1 3707868..3709406 (+) 1539 WP_250198281.1 RNA polymerase factor sigma-54 Machinery gene
  MW290_RS31560 (MW290_31555) - 3709407..3710642 (+) 1236 WP_250198282.1 class I SAM-dependent RNA methyltransferase -
  MW290_RS31565 (MW290_31560) eutC 3710643..3711440 (-) 798 WP_250198283.1 ethanolamine ammonia-lyase subunit EutC -
  MW290_RS31570 (MW290_31565) - 3711433..3712830 (-) 1398 WP_250198284.1 ethanolamine ammonia-lyase subunit EutB -
  MW290_RS31575 (MW290_31570) eat 3712850..3714247 (-) 1398 WP_250198285.1 ethanolamine permease -

Sequence

Protein


Download         Length: 512 a.a.        Molecular weight: 56192.36 Da        Isoelectric Point: 4.6493

>NTDB_id=691379 MW290_RS31555 WP_250198281.1 3707868..3709406(+) (rpoN/rpoN1) [Aquincola tertiaricarbonis strain RN12]
MKPSLQIRLSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLEQNPFLETEEEHVPAFEQSLERPAASSNADDGDYAGSSS
TETGAAADGDGAEAPGVDSADFGTTERDDWENGTERDDFDGIRETPSASSSSNNDSDDFEPQERGGGGISLQEHLRAQMA
GMRLEPADAAALHVLIESLNEDGYLADPLAEIAAQLAGDADLEQQEELLERLQCALKWLQSLEPLGVGARDLPECLVLQL
KALPRSEAQAVAIIVCKQHLELLARRDLKKLIAATGADEELLKAAQELIRRCEPKPGRPFARAEANIIIPDVIVQRNGRG
WKVVLNPDVMPKLRINDLYAQAIRGNRGQNGNGALGARLQEARWFMKNIQQRFDTILRVSSAIVERQKNFFTHGEIAMKP
LVLREIADELGLHESTISRVTTAKYMATMFGTFELKYFFGSSLNTDAGGNASSTAVRALIKQLVAAEDAAKPLSDSQLSQ
MLEEQGIQVARRTVAKYREALKIAPANLRKAM

Nucleotide


Download         Length: 1539 bp        

>NTDB_id=691379 MW290_RS31555 WP_250198281.1 3707868..3709406(+) (rpoN/rpoN1) [Aquincola tertiaricarbonis strain RN12]
ATGAAACCCTCGCTGCAAATCCGCCTCTCGCAGCACCTGGCCCTGACGCCACAGCTGCAGCAGTCCATCCGGCTGCTGCA
GCTGTCCACCCTGGAACTGCACCAGGAAGTCGAGCAGATGCTCGAGCAGAACCCCTTCCTGGAGACCGAGGAAGAGCATG
TGCCGGCCTTCGAGCAGTCGCTCGAGCGGCCGGCCGCCAGCAGCAATGCCGACGACGGCGACTACGCCGGCAGCAGCAGC
ACCGAAACCGGCGCCGCCGCCGACGGTGACGGCGCCGAGGCGCCCGGCGTGGACAGCGCCGACTTCGGCACCACCGAGCG
CGACGACTGGGAAAACGGCACCGAGCGCGACGACTTCGACGGCATCCGCGAAACGCCCTCGGCCAGCAGCAGCAGCAACA
ACGACAGCGACGACTTCGAGCCGCAGGAGCGCGGTGGCGGCGGCATCTCGCTGCAGGAGCACCTGCGCGCGCAGATGGCC
GGCATGCGCCTGGAGCCGGCCGACGCCGCCGCGCTGCATGTGCTGATCGAATCGCTGAACGAAGACGGCTACCTGGCCGA
CCCGCTGGCCGAGATCGCGGCCCAGCTGGCCGGCGATGCCGACCTGGAGCAGCAGGAAGAGCTGCTGGAGCGGCTGCAGT
GCGCGCTCAAGTGGCTGCAAAGCCTGGAGCCGCTGGGCGTGGGCGCGCGCGACCTGCCCGAATGCCTGGTGTTGCAGCTC
AAGGCGCTGCCGCGCAGCGAAGCACAGGCGGTGGCCATCATCGTGTGCAAGCAGCACCTGGAGCTGCTGGCGCGCCGCGA
CCTGAAGAAGCTGATCGCCGCCACCGGCGCCGACGAGGAGCTGCTGAAGGCCGCGCAGGAGCTGATCCGCCGCTGCGAGC
CCAAGCCCGGCCGGCCCTTCGCACGGGCCGAGGCCAACATCATCATTCCGGACGTGATCGTGCAGCGCAACGGCCGCGGC
TGGAAGGTGGTGCTCAACCCCGACGTGATGCCCAAGCTGCGCATCAACGACCTGTATGCGCAGGCCATCCGCGGCAACCG
CGGCCAGAACGGCAACGGCGCGCTGGGCGCGCGGCTGCAGGAGGCGCGCTGGTTCATGAAGAACATCCAGCAGCGCTTCG
ACACCATCCTGCGGGTGTCCAGCGCCATCGTCGAGCGGCAGAAGAACTTCTTCACCCATGGCGAGATCGCGATGAAGCCG
CTGGTGCTGCGCGAGATCGCCGACGAGCTGGGCCTGCACGAGTCCACCATCTCGCGCGTGACCACCGCCAAGTACATGGC
CACGATGTTCGGCACCTTCGAGCTGAAGTACTTCTTCGGCTCGTCGCTCAACACCGATGCCGGCGGCAATGCCTCCAGCA
CCGCGGTGCGCGCGCTCATCAAACAGCTGGTGGCGGCCGAGGACGCGGCCAAGCCGCTGTCGGACAGCCAGCTCAGCCAG
ATGCTGGAAGAACAAGGCATCCAGGTGGCGCGCCGCACCGTCGCCAAGTACCGTGAAGCCCTGAAGATCGCGCCGGCCAA
CCTGCGCAAGGCCATGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(357-510)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(149-339)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S6R6J6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

54.943

100

0.564