Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   M8965_RS13295 Genome accession   NZ_CP097602
Coordinates   2597790..2598404 (-) Length   204 a.a.
NCBI ID   WP_032873955.1    Uniprot ID   -
Organism   Bacillus velezensis strain UA0244     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2592790..2603404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8965_RS13265 rpsT 2593093..2593359 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  M8965_RS13270 holA 2593376..2594417 (-) 1042 Protein_2609 DNA polymerase III subunit delta -
  M8965_RS13275 - 2594457..2594608 (-) 152 Protein_2610 hypothetical protein -
  M8965_RS13280 - 2594649..2594783 (+) 135 WP_003152870.1 YqzM family protein -
  M8965_RS13285 comEC 2594802..2597153 (-) 2352 WP_032873958.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  M8965_RS13290 - 2597154..2597723 (-) 570 WP_003152868.1 ComE operon protein 2 -
  M8965_RS13295 comEA 2597790..2598404 (-) 615 WP_032873955.1 helix-hairpin-helix domain-containing protein Machinery gene
  M8965_RS13300 comER 2598463..2599284 (+) 822 WP_012118027.1 late competence protein ComER -
  M8965_RS13305 - 2599353..2600090 (-) 738 WP_032873951.1 class I SAM-dependent methyltransferase -
  M8965_RS13310 rsfS 2600087..2600443 (-) 357 WP_007408260.1 ribosome silencing factor -
  M8965_RS13315 yqeK 2600447..2601022 (-) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  M8965_RS13320 - 2601012..2601581 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  M8965_RS13325 yhbY 2601592..2601882 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  M8965_RS13330 aroE 2601876..2602718 (-) 843 WP_032873946.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22265.47 Da        Isoelectric Point: 6.4940

>NTDB_id=691030 M8965_RS13295 WP_032873955.1 2597790..2598404(-) (comEA) [Bacillus velezensis strain UA0244]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=691030 M8965_RS13295 WP_032873955.1 2597790..2598404(-) (comEA) [Bacillus velezensis strain UA0244]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363