Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   M8968_RS09435 Genome accession   NZ_CP097594
Coordinates   1835192..1835806 (+) Length   204 a.a.
NCBI ID   WP_032873955.1    Uniprot ID   -
Organism   Bacillus velezensis strain UA0182     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1830192..1840806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8968_RS09400 aroE 1830878..1831720 (+) 843 WP_032873946.1 shikimate dehydrogenase -
  M8968_RS09405 yhbY 1831714..1832004 (+) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  M8968_RS09410 - 1832015..1832584 (+) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  M8968_RS09415 yqeK 1832574..1833149 (+) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  M8968_RS09420 rsfS 1833153..1833509 (+) 357 WP_007408260.1 ribosome silencing factor -
  M8968_RS09425 - 1833506..1834243 (+) 738 WP_032873951.1 class I SAM-dependent methyltransferase -
  M8968_RS09430 comER 1834312..1835133 (-) 822 WP_012118027.1 late competence protein ComER -
  M8968_RS09435 comEA 1835192..1835806 (+) 615 WP_032873955.1 helix-hairpin-helix domain-containing protein Machinery gene
  M8968_RS09440 - 1835873..1836442 (+) 570 WP_003152868.1 ComE operon protein 2 -
  M8968_RS09445 comEC 1836443..1838794 (+) 2352 WP_032873958.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  M8968_RS09450 - 1838813..1838947 (-) 135 WP_003152870.1 YqzM family protein -
  M8968_RS09455 - 1838988..1839139 (+) 152 Protein_1849 hypothetical protein -
  M8968_RS09460 holA 1839179..1840220 (+) 1042 Protein_1850 DNA polymerase III subunit delta -
  M8968_RS09465 rpsT 1840237..1840503 (-) 267 WP_003152876.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22265.47 Da        Isoelectric Point: 6.4940

>NTDB_id=690934 M8968_RS09435 WP_032873955.1 1835192..1835806(+) (comEA) [Bacillus velezensis strain UA0182]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=690934 M8968_RS09435 WP_032873955.1 1835192..1835806(+) (comEA) [Bacillus velezensis strain UA0182]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363