Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   M8969_RS14890 Genome accession   NZ_CP097593
Coordinates   3021223..3021837 (+) Length   204 a.a.
NCBI ID   WP_032873955.1    Uniprot ID   -
Organism   Bacillus velezensis strain UA0229     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3016223..3026837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8969_RS14855 aroE 3016909..3017751 (+) 843 WP_032873946.1 shikimate dehydrogenase -
  M8969_RS14860 yhbY 3017745..3018035 (+) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  M8969_RS14865 - 3018046..3018615 (+) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  M8969_RS14870 yqeK 3018605..3019180 (+) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  M8969_RS14875 rsfS 3019184..3019540 (+) 357 WP_007408260.1 ribosome silencing factor -
  M8969_RS14880 - 3019537..3020274 (+) 738 WP_032873951.1 class I SAM-dependent methyltransferase -
  M8969_RS14885 comER 3020343..3021164 (-) 822 WP_012118027.1 late competence protein ComER -
  M8969_RS14890 comEA 3021223..3021837 (+) 615 WP_032873955.1 helix-hairpin-helix domain-containing protein Machinery gene
  M8969_RS14895 - 3021904..3022473 (+) 570 WP_003152868.1 ComE operon protein 2 -
  M8969_RS14900 comEC 3022474..3024825 (+) 2352 WP_032873958.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  M8969_RS14905 - 3024844..3024978 (-) 135 WP_003152870.1 YqzM family protein -
  M8969_RS14910 - 3025019..3025170 (+) 152 Protein_2921 hypothetical protein -
  M8969_RS14915 holA 3025210..3026251 (+) 1042 Protein_2922 DNA polymerase III subunit delta -
  M8969_RS14920 rpsT 3026268..3026534 (-) 267 WP_003152876.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22265.47 Da        Isoelectric Point: 6.4940

>NTDB_id=690879 M8969_RS14890 WP_032873955.1 3021223..3021837(+) (comEA) [Bacillus velezensis strain UA0229]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=690879 M8969_RS14890 WP_032873955.1 3021223..3021837(+) (comEA) [Bacillus velezensis strain UA0229]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363