Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   M8970_RS07775 Genome accession   NZ_CP097590
Coordinates   1657164..1657778 (-) Length   204 a.a.
NCBI ID   WP_032873955.1    Uniprot ID   -
Organism   Bacillus velezensis strain UA0195     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1652164..1662778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8970_RS07745 rpsT 1652467..1652733 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  M8970_RS07750 holA 1652750..1653791 (-) 1042 Protein_1528 DNA polymerase III subunit delta -
  M8970_RS07755 - 1653831..1653982 (-) 152 Protein_1529 hypothetical protein -
  M8970_RS07760 - 1654023..1654157 (+) 135 WP_003152870.1 YqzM family protein -
  M8970_RS07765 comEC 1654176..1656527 (-) 2352 WP_032873958.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  M8970_RS07770 - 1656528..1657097 (-) 570 WP_003152868.1 ComE operon protein 2 -
  M8970_RS07775 comEA 1657164..1657778 (-) 615 WP_032873955.1 helix-hairpin-helix domain-containing protein Machinery gene
  M8970_RS07780 comER 1657837..1658658 (+) 822 WP_012118027.1 late competence protein ComER -
  M8970_RS07785 - 1658727..1659464 (-) 738 WP_032873951.1 class I SAM-dependent methyltransferase -
  M8970_RS07790 rsfS 1659461..1659817 (-) 357 WP_007408260.1 ribosome silencing factor -
  M8970_RS07795 yqeK 1659821..1660396 (-) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  M8970_RS07800 - 1660386..1660955 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  M8970_RS07805 yhbY 1660966..1661256 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  M8970_RS07810 aroE 1661250..1662092 (-) 843 WP_032873946.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22265.47 Da        Isoelectric Point: 6.4940

>NTDB_id=690711 M8970_RS07775 WP_032873955.1 1657164..1657778(-) (comEA) [Bacillus velezensis strain UA0195]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=690711 M8970_RS07775 WP_032873955.1 1657164..1657778(-) (comEA) [Bacillus velezensis strain UA0195]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363