Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   M8966_RS16540 Genome accession   NZ_CP097589
Coordinates   3337445..3338245 (+) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain UA0311     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 3332445..3343245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8966_RS16520 - 3332567..3333412 (+) 846 WP_007612503.1 TlyA family RNA methyltransferase -
  M8966_RS16525 argR 3333509..3333958 (+) 450 WP_003153173.1 transcriptional regulator ArgR -
  M8966_RS16530 recN 3333990..3335720 (+) 1731 WP_012117962.1 DNA repair protein RecN Machinery gene
  M8966_RS16535 spoIVB 3335884..3337164 (+) 1281 WP_012117961.1 SpoIVB peptidase -
  M8966_RS16540 spo0A 3337445..3338245 (+) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  M8966_RS16545 alr 3338394..3339575 (-) 1182 WP_012117960.1 alanine racemase -
  M8966_RS16550 - 3339752..3339964 (-) 213 WP_003153181.1 spore germination protein -
  M8966_RS16555 - 3340090..3340302 (-) 213 WP_007408362.1 spore germination protein -
  M8966_RS16560 - 3340430..3341548 (-) 1119 WP_015240192.1 NADH-dependent flavin oxidoreductase -
  M8966_RS16565 - 3341642..3341779 (-) 138 WP_014470646.1 hypothetical protein -
  M8966_RS16570 - 3342076..3342708 (-) 633 WP_007408365.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=690669 M8966_RS16540 WP_003153177.1 3337445..3338245(+) (spo0A) [Bacillus velezensis strain UA0311]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=690669 M8966_RS16540 WP_003153177.1 3337445..3338245(+) (spo0A) [Bacillus velezensis strain UA0311]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCAGATGTGCTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCCGCGCAAAGCAGTATTATCCGCAGCCAGCCGGAACCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACTGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAGCCAACCAACTCGGAATTCATCGCGATGGTTGCTGACAAGCTGAGATTAGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97