Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   M8966_RS13770 Genome accession   NZ_CP097589
Coordinates   2827915..2828682 (+) Length   255 a.a.
NCBI ID   WP_012118014.1    Uniprot ID   A7Z6U2
Organism   Bacillus velezensis strain UA0311     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2822915..2833682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8966_RS13740 - 2823199..2825331 (+) 2133 WP_007408284.1 HD family phosphohydrolase -
  M8966_RS13745 ybeY 2825332..2825805 (+) 474 WP_012118016.1 rRNA maturation RNase YbeY -
  M8966_RS13750 - 2825786..2826160 (+) 375 WP_015240233.1 diacylglycerol kinase family protein -
  M8966_RS13755 - 2826284..2826694 (+) 411 WP_003152976.1 cytidine deaminase -
  M8966_RS13760 era 2826675..2827580 (+) 906 WP_007408285.1 GTPase Era -
  M8966_RS13765 - 2827735..2827878 (+) 144 WP_007408286.1 YqzL family protein -
  M8966_RS13770 recO 2827915..2828682 (+) 768 WP_012118014.1 DNA repair protein RecO Machinery gene
  M8966_RS13775 glyQ 2828984..2829871 (+) 888 WP_003152984.1 glycine--tRNA ligase subunit alpha -
  M8966_RS13780 glyS 2829864..2831903 (+) 2040 WP_012118013.1 glycine--tRNA ligase subunit beta -
  M8966_RS13785 ccpN 2832004..2832642 (+) 639 WP_003152989.1 transcriptional regulator CcpN -
  M8966_RS13790 - 2832668..2833480 (+) 813 WP_003152991.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29310.95 Da        Isoelectric Point: 8.5625

>NTDB_id=690647 M8966_RS13770 WP_012118014.1 2827915..2828682(+) (recO) [Bacillus velezensis strain UA0311]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNDGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSGEG
TFHFSIRDNGFICHRCFEKDPYRVPMSPQTARLLRLFYYFDIGRLGSVSLKQETKNEIKRVIDLYYEEYSGLYLKSKRFL
DQMESMKNLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=690647 M8966_RS13770 WP_012118014.1 2827915..2828682(+) (recO) [Bacillus velezensis strain UA0311]
ATGCTGACTAAATGTGAAGGAATCGTGCTCCGTACGAATGACTACGGGGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTCATGGCAAGGGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGATTTATTTTTGACGGCGTATGCCGCTTTTATCGCTGAACTCACTGACAAAGGAACGGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGACGGGACTGACCCGGACGTGATCACCTTCA
TCGTGCAGATGAAAATGCTCGGCGTCATGGGGCTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCGGGGAAGGG
ACGTTTCATTTTTCGATAAGAGATAACGGGTTTATCTGCCACCGCTGCTTTGAAAAGGATCCGTACAGAGTTCCGATGTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCGTTAAAACAGGAAA
CCAAAAACGAAATTAAACGGGTCATTGATCTGTATTACGAGGAATATTCAGGACTTTATTTAAAGTCAAAACGGTTTTTA
GATCAGATGGAAAGCATGAAGAACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7Z6U2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

88.627

100

0.886