Detailed information NTG0690286

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: OUY06_RS05265
• Genome accession: NZ_CP113247
• Coordinates: 1031554..1035288 (+)
• Length: 1244 a.a.
• NCBI ID: WP_226474277.1
• Uniprot ID: -
• Organism: Latilactobacillus sakei strain CNSC001WB
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1026554..1040288

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OUY06_RS05255	mvk	1026777..1027724 (-)	948	WP_025015793.1	mevalonate kinase	-
OUY06_RS05260	-	1028001..1031561 (+)	3561	WP_016265071.1	PD-(D/E)XK nuclease family protein	-
OUY06_RS05265	addA	1031554..1035288 (+)	3735	WP_226474277.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
OUY06_RS05270	-	1035354..1038188 (+)	2835	WP_016265073.1	helicase C-terminal domain-containing protein	-
OUY06_RS05275	-	1038266..1038742 (+)	477	WP_011374615.1	DUF5590 domain-containing protein	-
OUY06_RS05280	asnS	1038853..1040151 (+)	1299	WP_011374616.1	asparagine--tRNA ligase	-

Sequence

Protein

• Length: 1244 a.a.
• Molecular weight: 141274.03 Da
• Isoelectric Point: 4.7686

>NTDB_id=690286 OUY06_RS05265 WP_226474277.1 1031554..1035288(+) (addA) [Latilactobacillus sakei strain CNSC001WB]
MSNPQFTPSQQAAVDHDGHDILVSASAGSGKTSVLVARVIQKILNGTDVDTLLVVTFTEAAATEMRQRIQVALRDASEKA
TEPAVKQRLRQQLSLVPTAQISTLHAFCLKVIKQFYYVIDRDPVFRLLSDTAERLLLADQVWQRVREAFYNHEYVKENEQ
DTLFYELAQNFSNDRNDDGLTDIVFELLDFANANPNPVKWLNNLPKSYAVDEAGLTASDYFQKKILPILQQTITECLEAL
QEAEQLSQTSENLMIYAPQIATTQAALTQINADAKTLNWQAWRQQLTDAQLGPAKRTKKLEPDEQINKDRLKADLDDVKK
KIQTLLDTYFVFDEATTANIQRQAGQLVQKLVSVTLTFRAAFQAEKERRHLLDFSDLEQLCLTILSAEDSPARAFYQQKF
SEVLVDEYQDTNPLQETIIQKVTSDHPRNLFMVGDVKQSIYAFRLADPSLFKNKYNEFGVTEAERDSERIILAENFRSRR
NIDDFTNLIFKQLMDENLGELDYDENAALQYGARYYPDQHPTAPTELLLYETKPEEAVANPQLDKSEGQVVAVAKRIQAM
MTNGEQIWDKKLEKMRPIEYRDIVLLAPTRGNNLFILDYFKRFGLPVVIKDAQNYFQTTEVQIMLALLQVIDNPNQDIPL
VSVLRSPIVGLNENELALIRINDKTDDYYQAVFNFIDQYNEQKVGHLGQQVMQKLTHFMALLTSFRTIARQQPIVDLIWT
IYQETGFLDYVGGMPAGRQRQANLHALYERATAYEENGFKGLFQFIQFIERLQKQDKDLAQPTSLENQDAISVMTIHGSK
GLEFPVVFLIDTSRRFNQQDLQRSYVLDNHGGLGVVYLDSQKRLKVPTLPELAITAQKRKKLRAEEMRKLYVALTRAEQR
LIIVGHCDNQAQLMKQWQKGQNSTQQILDDGVRNDAASLLDWIGLSLIRHPQAKDWTTDYEPLLALSGDQTEFKLIMTNE
TLLGEIPGSLHNDQDWGQQQQKALQTIDLNSGIATAIAEQLTFKYPANVATQTTAYQSVSEVKRLFEDPDNTGLGQLDLA
AEQPRQANRYVTDSLAQPQFLQTVQQPTAAEVGTATHLVLQEIDLTQPVTLASLQALVDRLVLQNSLSANVAAKINLNHL
LTFFESDLGQQLLAHPEAVKREVPFSLLLPAETIFEGIRPTDDDHVLIHGIMDGYLLTDDGCYLFDYKTDFIDPSDQEAA
IERVKTRYAGQINLYSAALNQIAQKPVTHKYLYLLSIGELVEIN

Nucleotide

• Length: 3735 bp

>NTDB_id=690286 OUY06_RS05265 WP_226474277.1 1031554..1035288(+) (addA) [Latilactobacillus sakei strain CNSC001WB]
TTGAGTAACCCCCAATTTACACCTTCACAGCAAGCCGCTGTCGATCATGATGGCCACGATATTTTAGTATCCGCATCAGC
TGGTTCAGGGAAGACATCCGTTCTTGTTGCGCGGGTCATCCAAAAAATCCTAAACGGGACCGATGTCGATACCTTACTTG
TTGTGACGTTTACGGAAGCCGCTGCTACTGAAATGCGCCAACGGATTCAAGTAGCACTCCGCGACGCCAGTGAGAAAGCA
ACAGAACCAGCTGTTAAGCAACGCTTACGGCAACAATTAAGTCTCGTGCCTACCGCACAAATTAGTACACTGCATGCCTT
TTGTTTAAAAGTCATTAAACAATTTTATTATGTCATTGATCGTGATCCCGTATTCCGCTTATTGAGTGATACCGCAGAAC
GACTCCTATTAGCGGATCAAGTATGGCAACGGGTGAGAGAGGCCTTCTATAATCACGAATATGTCAAAGAAAATGAGCAA
GATACATTATTCTACGAATTAGCCCAAAATTTCTCGAATGATCGTAACGATGACGGCCTGACCGACATTGTTTTTGAGTT
GCTCGATTTTGCAAACGCCAATCCAAACCCGGTTAAATGGTTAAATAACCTTCCCAAAAGTTATGCCGTTGACGAAGCTG
GTTTGACGGCCAGTGATTATTTCCAAAAAAAAATATTACCGATTTTACAGCAAACCATCACCGAATGTTTGGAAGCCTTA
CAAGAAGCTGAACAGCTCAGTCAAACGAGCGAAAATCTAATGATCTATGCCCCTCAAATTGCAACTACGCAAGCAGCCTT
AACACAAATTAACGCAGATGCAAAAACGTTGAATTGGCAAGCATGGCGCCAACAACTAACAGATGCACAGCTGGGGCCTG
CTAAACGGACTAAGAAATTAGAACCCGACGAACAAATTAATAAGGACCGGTTGAAAGCCGATTTAGATGACGTCAAAAAG
AAAATTCAGACGTTACTGGACACCTATTTTGTGTTTGATGAAGCAACCACCGCTAATATTCAACGTCAAGCTGGCCAATT
GGTTCAAAAATTGGTGAGCGTCACGTTAACTTTTAGAGCAGCCTTTCAAGCCGAAAAAGAACGACGTCATTTATTGGACT
TTAGTGATTTAGAACAACTTTGTCTGACAATTTTATCGGCAGAGGATTCGCCGGCAAGGGCCTTTTACCAACAAAAATTT
AGCGAAGTGCTAGTTGATGAATATCAAGATACCAATCCGCTCCAAGAAACGATTATCCAAAAGGTCACTTCTGATCATCC
ACGCAATCTCTTCATGGTCGGGGATGTTAAGCAGTCAATTTATGCTTTCCGATTGGCCGATCCAAGCTTATTTAAGAATA
AGTATAACGAATTTGGTGTCACGGAAGCTGAACGTGATAGTGAACGAATCATCTTAGCTGAAAACTTCCGTTCGCGCCGC
AATATCGATGACTTTACCAATCTGATTTTTAAACAATTGATGGACGAAAATCTCGGCGAGTTAGACTACGACGAAAATGC
GGCCTTACAATATGGTGCTCGTTATTATCCTGACCAGCACCCAACCGCGCCAACCGAACTCTTGCTTTATGAAACTAAGC
CGGAAGAGGCAGTTGCCAACCCGCAATTAGATAAGAGTGAGGGCCAAGTCGTGGCGGTCGCTAAACGGATTCAAGCCATG
ATGACTAATGGGGAACAGATTTGGGATAAGAAACTCGAAAAAATGCGGCCGATTGAATATCGGGACATCGTTTTACTAGC
CCCAACCCGTGGTAATAATTTGTTTATCCTGGATTATTTCAAACGCTTCGGTTTACCGGTTGTGATTAAGGATGCGCAAA
ACTATTTCCAAACGACCGAAGTCCAAATTATGTTAGCTCTACTTCAGGTGATTGATAATCCTAACCAAGATATTCCATTG
GTCAGTGTTTTACGCTCACCAATTGTCGGGTTAAACGAAAATGAATTGGCATTAATTCGGATTAACGATAAGACGGATGA
TTATTATCAAGCGGTCTTTAACTTTATTGACCAATATAACGAGCAGAAAGTCGGGCATTTAGGCCAACAGGTCATGCAGA
AGCTGACGCACTTCATGGCTTTATTGACGTCGTTTAGAACAATTGCTCGCCAACAACCGATTGTGGACTTAATTTGGACG
ATCTACCAAGAAACTGGCTTTTTAGACTATGTCGGTGGGATGCCAGCTGGACGTCAACGTCAAGCTAATTTACACGCGCT
TTATGAACGTGCCACGGCGTATGAAGAAAATGGCTTTAAAGGACTCTTCCAATTTATTCAATTTATTGAACGTCTCCAGA
AACAAGATAAGGATTTAGCACAACCAACGAGCTTAGAAAATCAGGACGCCATCTCTGTTATGACCATTCACGGGAGTAAG
GGGCTTGAATTTCCAGTCGTCTTTTTAATCGATACGAGTCGTCGCTTTAACCAACAGGATTTACAACGCTCATATGTTTT
GGATAATCATGGTGGTTTAGGGGTCGTTTATTTAGATAGCCAAAAGCGGTTGAAGGTGCCAACACTGCCAGAGTTAGCGA
TTACCGCCCAGAAACGAAAAAAATTACGAGCAGAAGAAATGCGAAAACTATACGTTGCTTTGACACGTGCGGAACAACGG
TTAATCATCGTTGGCCATTGTGATAACCAGGCCCAATTGATGAAGCAATGGCAAAAAGGGCAAAATAGTACCCAACAAAT
ATTAGATGATGGGGTTCGCAACGATGCCGCAAGTTTACTGGACTGGATAGGTCTTAGCTTAATTCGCCATCCACAAGCTA
AAGATTGGACCACTGATTATGAGCCACTACTGGCTTTAAGTGGTGATCAAACCGAATTTAAGTTGATTATGACTAATGAA
ACCTTACTTGGTGAAATACCAGGTAGTCTGCATAACGATCAGGACTGGGGACAACAACAGCAAAAGGCACTTCAAACAAT
TGATTTAAACAGTGGGATAGCAACTGCGATTGCTGAGCAATTAACCTTTAAGTATCCCGCTAATGTTGCCACCCAAACGA
CGGCTTATCAATCTGTTTCGGAAGTCAAACGCCTTTTTGAAGACCCTGATAATACCGGGTTGGGACAATTAGACCTAGCG
GCTGAACAACCGCGCCAAGCAAATCGCTACGTAACGGATAGCTTAGCGCAACCACAGTTCTTACAAACCGTTCAACAACC
AACGGCAGCAGAAGTAGGGACCGCAACGCATTTGGTCTTGCAAGAAATTGATTTAACGCAACCAGTAACGCTAGCGAGTT
TGCAAGCCCTTGTCGATCGACTTGTTTTACAGAATAGTTTAAGCGCCAATGTTGCGGCTAAGATTAATCTTAATCACCTA
TTAACCTTCTTTGAAAGTGACTTGGGACAGCAATTATTAGCCCATCCAGAAGCGGTTAAGCGGGAAGTGCCATTTTCGTT
GTTATTACCAGCAGAAACGATTTTTGAAGGCATTCGTCCCACAGATGACGATCACGTCCTAATCCATGGGATTATGGATG
GTTATTTATTGACTGACGACGGTTGTTATTTATTTGATTACAAAACCGACTTTATTGACCCCAGTGATCAAGAAGCGGCG
ATTGAACGGGTTAAAACCCGCTATGCTGGCCAGATTAACCTCTATAGTGCTGCTTTAAACCAGATTGCCCAAAAGCCAGT
GACCCATAAATATCTCTATTTATTGTCAATTGGCGAGTTGGTTGAGATTAACTAG

Domains

Predicted by InterProScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1064-1227)

• UvrD-like helicase C-terminal domain (UvrD_C) (470-884)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (7-458)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	37.51	100	0.383