Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   N508_RS03255 Genome accession   NZ_CP097562
Coordinates   684439..685782 (-) Length   447 a.a.
NCBI ID   WP_023274968.1    Uniprot ID   V2QGZ2
Organism   Mucispirillum schaedleri ASF457     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 679439..690782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N508_RS03235 (N508_000655) - 680364..680930 (-) 567 Protein_631 restriction endonuclease subunit S -
  N508_RS03240 (N508_000656) - 680942..681562 (-) 621 WP_251930658.1 restriction endonuclease subunit S -
  N508_RS03245 (N508_000657) - 681566..682585 (-) 1020 WP_023274965.1 virulence RhuM family protein -
  N508_RS10910 (N508_000658) - 682733..682858 (-) 126 WP_023274966.1 hypothetical protein -
  N508_RS03250 (N508_000659) - 682859..684424 (-) 1566 WP_023274967.1 type I restriction-modification system subunit M -
  N508_RS03255 (N508_000660) radA 684439..685782 (-) 1344 WP_023274968.1 DNA repair protein RadA Machinery gene
  N508_RS03260 (N508_000661) ruvB 685782..686810 (-) 1029 WP_023274969.1 Holliday junction branch migration DNA helicase RuvB -
  N508_RS03265 (N508_000662) ruvA 686810..687382 (-) 573 WP_023274970.1 Holliday junction branch migration protein RuvA -
  N508_RS03270 (N508_000663) ruvC 687405..687893 (-) 489 WP_023274971.1 crossover junction endodeoxyribonuclease RuvC -
  N508_RS03275 (N508_000664) - 687900..688643 (-) 744 WP_023274972.1 YebC/PmpR family DNA-binding transcriptional regulator -
  N508_RS03280 (N508_000665) - 689175..689474 (+) 300 WP_023274973.1 HNH endonuclease -
  N508_RS03285 (N508_000666) - 689553..690002 (+) 450 WP_023274974.1 hypothetical protein -
  N508_RS03290 (N508_000667) - 690008..690604 (+) 597 WP_023274975.1 hypothetical protein -

Sequence

Protein


Download         Length: 447 a.a.        Molecular weight: 48628.83 Da        Isoelectric Point: 8.1234

>NTDB_id=690163 N508_RS03255 WP_023274968.1 684439..685782(-) (radA) [Mucispirillum schaedleri ASF457]
MAKLKTTYVCQNCGAVQSKWMGKCPECNQWNSLVEEVIEKQSSSKSSYNQNYIPKNTPVILSNVAGIETHRFETGIKELD
QVLGGGIVKGSVVLIGGEPGIGKSTVILQVAGILNKKNKKVLYVSGEESPSQIRMRADRLNVTADNLELLTTTSFEDVLS
ATAIKQYDYLIIDSIQTITSEEIISGGGAVGQIRHVTYRLVELAKSTGLTVFIIGQVTKDGNIAGPKVLEHLVDTVLYFE
GDYSRGVRIIRSVKNRFGSTDEVGIFEMSQSGLIEAGDRAFFDKDSSQASGKVICPVLEGTRPFLIEIQALVAPTFFQYP
KRNATGYDNNRLAMLIAILEKKGGLNLSGADVYLNIAGGIKINEPSADLAVCAALISSFKDKPVPYTMAFAGEVGLTGEI
RSVSNIQGRINECSKFGVETLYTNNKHEKSKNIKLVNVKNINDLLKL

Nucleotide


Download         Length: 1344 bp        

>NTDB_id=690163 N508_RS03255 WP_023274968.1 684439..685782(-) (radA) [Mucispirillum schaedleri ASF457]
ATGGCAAAATTAAAAACAACTTATGTATGCCAGAACTGTGGAGCAGTCCAGTCTAAATGGATGGGAAAATGTCCAGAATG
TAATCAGTGGAATTCGCTGGTAGAAGAAGTTATTGAAAAGCAGTCATCATCAAAATCATCTTATAATCAAAACTATATTC
CTAAAAACACACCTGTTATTTTATCAAATGTGGCAGGTATAGAAACTCACCGCTTTGAAACAGGCATAAAAGAGCTGGAT
CAGGTATTAGGTGGCGGTATAGTTAAAGGTTCTGTTGTTTTAATAGGCGGCGAGCCGGGTATTGGCAAATCTACTGTTAT
ATTGCAGGTTGCAGGCATATTAAATAAGAAAAATAAAAAAGTGCTTTATGTATCAGGTGAAGAATCACCATCACAAATCC
GTATGCGGGCAGACAGACTAAATGTTACAGCTGATAATTTAGAGCTTTTAACAACTACTTCTTTTGAAGATGTGCTTTCT
GCTACAGCTATTAAACAGTATGATTATTTAATAATAGATTCTATACAGACAATTACAAGTGAAGAAATTATTTCAGGCGG
CGGTGCAGTAGGGCAGATTCGCCATGTTACATACAGGCTTGTAGAGCTTGCAAAAAGCACTGGTCTTACTGTCTTTATTA
TAGGGCAGGTAACAAAAGACGGCAATATTGCAGGTCCGAAAGTATTAGAACATTTAGTTGATACTGTTCTTTATTTTGAG
GGCGATTATTCAAGGGGAGTCCGCATAATCCGCTCTGTTAAAAACAGGTTTGGCTCAACTGATGAAGTGGGTATTTTTGA
AATGAGCCAGTCTGGTTTAATAGAAGCAGGGGACAGAGCCTTTTTTGATAAAGACAGCAGTCAGGCAAGCGGCAAAGTAA
TATGCCCTGTGTTAGAGGGCACAAGACCTTTTTTAATAGAAATACAGGCACTTGTTGCACCAACATTTTTTCAATACCCT
AAAAGAAATGCCACAGGCTATGACAACAACAGGCTTGCAATGCTTATTGCTATTTTAGAAAAAAAGGGCGGCTTAAACTT
ATCTGGTGCAGATGTTTATTTAAATATTGCAGGTGGAATTAAAATCAATGAGCCAAGTGCAGATTTAGCAGTATGTGCTG
CCCTTATTTCATCATTTAAAGATAAGCCAGTGCCATATACAATGGCTTTTGCAGGGGAAGTTGGCTTGACTGGAGAAATC
CGCTCTGTTTCCAATATACAAGGCAGAATAAATGAATGTTCTAAATTTGGAGTGGAAACTTTATATACTAATAATAAACA
TGAAAAAAGTAAAAATATAAAATTAGTTAATGTGAAAAACATAAATGATTTATTAAAACTATAA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(357-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

KaiC (ATPase)

(73-160)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V2QGZ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae D39

47.788

100

0.483
  radA Streptococcus mitis NCTC 12261

47.788

100

0.483
  radA Streptococcus pneumoniae Rx1

47.788

100

0.483
  radA Streptococcus pneumoniae R6

47.788

100

0.483
  radA Streptococcus pneumoniae TIGR4

47.788

100

0.483
  radA Streptococcus mitis SK321

47.566

100

0.481
  radA Bacillus subtilis subsp. subtilis str. 168

45.475

100

0.461