Detailed information NTG0689994

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M8333_RS23520
• Genome accession: NZ_CP097554
• Coordinates: 4983000..4983797 (+)
• Length: 265 a.a.
• NCBI ID: WP_014227970.1
• Uniprot ID: A0A0H3H8P5
• Organism: Klebsiella michiganensis strain YZUMF202001
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4978000..4988797

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M8333_RS23485 (M8333_23480)	gspH	4978141..4978653 (+)	513	WP_048262354.1	type II secretion system minor pseudopilin GspH	-
M8333_RS23490 (M8333_23485)	gspI	4978650..4979015 (+)	366	WP_048262356.1	type II secretion system minor pseudopilin GspI	-
M8333_RS23495 (M8333_23490)	gspJ	4979012..4979608 (+)	597	WP_048262357.1	type II secretion system minor pseudopilin GspJ	-
M8333_RS23500 (M8333_23495)	gspK	4979605..4980585 (+)	981	WP_048262360.1	type II secretion system minor pseudopilin GspK	-
M8333_RS23505 (M8333_23500)	gspL	4980578..4981771 (+)	1194	WP_025107363.1	type II secretion system protein GspL	-
M8333_RS23510 (M8333_23505)	gspM	4981764..4982249 (+)	486	WP_025107362.1	type II secretion system protein GspM	-
M8333_RS23515 (M8333_23510)	-	4982239..4982997 (+)	759	WP_064405742.1	type II secretion system protein N	-
M8333_RS23520 (M8333_23515)	pilD	4983000..4983797 (+)	798	WP_014227970.1	prepilin peptidase	Machinery gene
M8333_RS23525 (M8333_23520)	hrpB	4984159..4986588 (-)	2430	WP_049102372.1	ATP-dependent helicase HrpB	-
M8333_RS23530 (M8333_23525)	thpR	4986661..4987197 (+)	537	WP_014837425.1	RNA 2',3'-cyclic phosphodiesterase	-
M8333_RS23535 (M8333_23530)	sfsA	4987197..4987913 (+)	717	WP_275050591.1	DNA/RNA nuclease SfsA	-
M8333_RS23540 (M8333_23535)	dksA	4988078..4988533 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29166.77 Da
• Isoelectric Point: 8.3358

>NTDB_id=689994 M8333_RS23520 WP_014227970.1 4983000..4983797(+) (pilD) [Klebsiella michiganensis strain YZUMF202001]
MENIALLPEFAAQYPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLWL
RGRARCCGQPISRRYPLMELTTGALFVLAGYLMAPGMPLLGGLIFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFAPLAEAVIGAMVGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=689994 M8333_RS23520 WP_014227970.1 4983000..4983797(+) (pilD) [Klebsiella michiganensis strain YZUMF202001]
ATGGAAAATATCGCGCTGTTACCCGAGTTCGCCGCGCAATACCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTGAATCTCTGCC
TCCCCGCGTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGCTTTTTATGGCTC
AGGGGGCGCGCGCGCTGCTGCGGGCAGCCGATCTCGCGTCGCTATCCCCTGATGGAGCTGACGACCGGCGCGCTGTTTGT
ACTCGCGGGGTACCTGATGGCTCCGGGCATGCCGCTGCTGGGCGGGCTAATTTTCGTGTCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGGCTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGTCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCGGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGCCTGGTAACCCGTCGT
TCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0H3H8P5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.708	100	0.498
pilD	Neisseria gonorrhoeae MS11	44.604	100	0.468
pilD	Vibrio campbellii strain DS40M4	43.636	100	0.453
pilD	Acinetobacter nosocomialis M2	44.788	97.736	0.438
pilD	Acinetobacter baumannii D1279779	44.402	97.736	0.434