Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   OTU45_RS03420 Genome accession   NZ_CP113114
Coordinates   678295..680517 (+) Length   740 a.a.
NCBI ID   WP_001809966.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain NP1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 673295..685517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OTU45_RS03395 (OTU45_03395) - 673762..674952 (-) 1191 WP_000843710.1 ferric reductase-like transmembrane domain-containing protein -
  OTU45_RS03400 (OTU45_03400) - 675097..675756 (-) 660 WP_000443860.1 amino acid ABC transporter permease -
  OTU45_RS03405 (OTU45_03405) - 675766..676443 (-) 678 WP_000131140.1 amino acid ABC transporter permease -
  OTU45_RS03410 (OTU45_03410) - 676456..677220 (-) 765 WP_000624332.1 transporter substrate-binding domain-containing protein -
  OTU45_RS03415 (OTU45_03415) - 677260..678018 (-) 759 WP_001229597.1 amino acid ABC transporter ATP-binding protein -
  OTU45_RS03420 (OTU45_03420) recJ 678295..680517 (+) 2223 WP_001809966.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  OTU45_RS03425 (OTU45_03425) - 680692..682353 (+) 1662 WP_000065636.1 ribonuclease J -
  OTU45_RS03430 (OTU45_03430) - 682422..683201 (+) 780 WP_267750161.1 alpha/beta hydrolase -
  OTU45_RS03435 (OTU45_03435) murM 683313..684533 (+) 1221 WP_000286563.1 peptidoglycan bridge formation alanyltransferase MurM -

Sequence

Protein


Download         Length: 740 a.a.        Molecular weight: 82507.00 Da        Isoelectric Point: 4.3320

>NTDB_id=689657 OTU45_RS03420 WP_001809966.1 678295..680517(+) (recJ) [Streptococcus pneumoniae strain NP1]
MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEE
GENILVYGDYDADGMTSASIVKESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQVELLDLVAIGTIADMVSLTDENR
ILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEETVGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKN
EERKEIVQSIYEEAKTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAVDIFEALDP
YRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNLDEELDLEALSLETVKSFERLAPFGMDNQKP
IFYIKNFQVESARTMGAGNAHLKLKISKGEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVE
GVQLFNIRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKNDIDKAYYLTGYGTRDQ
FAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFEELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKD
QEMMALGTVQEIYDFLMEKE

Nucleotide


Download         Length: 2223 bp        

>NTDB_id=689657 OTU45_RS03420 WP_001809966.1 678295..680517(+) (recJ) [Streptococcus pneumoniae strain NP1]
TTGATAACACCTACTTATGAATGGCAGTTTGCCCTGCAGGTAGAAGATGCGGATTTTACAAAGATAGCCAAGAAGGCTGG
ACTGGGTCCTGAGGTGGCTCGGTTATTGTTTGAGAGAGGGATTCAGGACCAAGAAAGTCTGAAGAAGTTTTTAGAACCTT
CCTTGGAGGACTTACATGATGCTTATCTGCTCCATGATATGGACAAGGCAGTGGAGCGGATTCGTCAGGCTATTGAAGAA
GGGGAAAATATTCTCGTTTATGGAGACTATGATGCGGATGGCATGACTTCGGCTTCTATTGTGAAGGAAAGTTTGGAACA
ACTTGGTGCTGAGTGCCGAGTTTACCTGCCAAATCGTTTTACCGATGGCTATGGCCCTAATGCTAGTGTTTATAAATACT
TTATCGAGCAAGAAGGAATTTCCTTGATTGTGACGGTGGACAATGGGGTTGCTGGTCATGAGGCTATTGCATTGGCTCAG
TCTATGGGAGTAGATGTCATTGTGACAGACCATCATTCCATGCCTGAAACCCTGCCAGATGCTTATGCTATTGTCCATCC
TGAACATCCAGATGCGGATTATCCTTTTAAATATTTGGCTGGTTGTGGAGTTGCTTTCAAGTTGGCTTGTGCCCTGTTAG
AAGAAGTGCAAGTGGAATTGCTTGATTTGGTCGCTATTGGAACTATTGCAGATATGGTGAGTCTGACGGATGAAAATCGT
ATCTTAGTTCAATATGGTCTGGAAATGTTGGGTCATACCCAGCGCATTGGTCTGCAAGAAATGCTGGACATGGCTGGGAT
TGCTGCCAACGAAGTAACAGAAGAAACGGTTGGTTTCCAGATTGCTCCTCGTTTGAATGCCTTGGGTCGCTTGGATGATC
CCAATCCTGCCATTGATTTGTTGACTGGATTTGATGATGAGGAAGCGCATGAGATTGCCCTTATGATTCACCAGAAAAAC
GAAGAGCGCAAGGAAATCGTTCAGTCTATCTATGAAGAAGCCAAGACCATGGTGGATCCTGAGAAGAAGGTTCAGGTCTT
GGCCAAGGAAGGCTGGAATCCTGGGGTTCTAGGAATCGTGGCTGGTCGTTTATTGGAAGAATTGGGACAGACAGTCATTG
TTCTTAATATAGAAGACGGTCGTGCCAAGGGCAGTGCTCGTAGTGTGGAAGCGGTCGATATTTTTGAAGCTCTGGATCCC
TATCGAGACCTCTTCATCGCCTTTGGAGGTCATGCAGGTGCAGCGGGTATGACGCTGGAAGTTGAGCAACTCTCAGATTT
ATCTCAGGTTTTGGAAGATTATGTTCGTGAAAAAGGTGCAGATGCTGGTGGCAAGAATAAGTTAAACCTAGATGAAGAGT
TGGATTTGGAGGCACTTAGCTTGGAAACGGTCAAAAGTTTTGAACGTTTAGCTCCTTTTGGAATGGATAATCAGAAACCT
ATTTTTTATATCAAGAATTTTCAGGTCGAAAGTGCTCGTACTATGGGGGCAGGTAATGCCCATCTAAAGCTGAAAATTTC
CAAGGGTGAGGCGAGTTTTGAAGTGGTAGCCTTTGGTCAAGGCAGATGGGCGACAGAGTTTTCTCAAACCAAGAATCTAG
AGTTAGCGGTTAAATTGTCTGTCAACCAATGGAATGGCCAAACTGCCCTCCAGTTGATGATGGTGGATGCGCGAGTGGAA
GGTGTTCAGCTTTTTAACATTCGTGGAAAAAATGCAGTCTTGCCAGAAGGTGTTCCAGTCTTGGATTTTCCTGGAGAACT
GCCAAATCTTGCGGCTAGTGAAGCTGTTGTCGTAAAAAACATTCCAGAGGATATTACTCAGCTGAAGACCATTTTTCAGG
AACAGCATTTCTCTGCTGTCTATTTCAAAAATGATATTGACAAGGCCTATTATCTGACAGGTTATGGGACTAGAGATCAG
TTTGCCAAATTGTACAAGACTATTTACCAGTTCCCAGAGTTTGATATTCGCTACAAGCTGAAAGATTTGGCTGCATATCT
TAATATTCAACAAATCTTGCTGGTCAAGATGATTCAAGTATTTGAAGAACTAGGCTTTGTGACGATAAAAGATGGTGTGA
TGACAGTCAATAAAGAGGCGCCAAAGCGGGAGATAGGAGAAAGTCAAATTTACCAAAATCTCAAACAAACCGTTAAAGAC
CAAGAAATGATGGCGCTGGGTACGGTGCAAGAAATTTATGATTTTTTGATGGAAAAAGAGTAG

Domains

Predicted by InterProScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(593-735)

DHHA1 domain (DHHA1)

(317-434)

RecJ OB domain (RecJ_OB)

(451-556)

DHH family (DHH)

(83-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

99.865

100

0.999
  recJ Bacillus subtilis subsp. subtilis str. 168

36.917

100

0.385