Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   M8X21_RS12180 Genome accession   NZ_CP097467
Coordinates   2508511..2509311 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain HMB26553     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 2503511..2514311
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8X21_RS12150 (M8X21_12150) - 2504059..2504691 (+) 633 WP_014418348.1 helix-turn-helix domain-containing protein -
  M8X21_RS12155 (M8X21_12155) - 2504982..2505119 (+) 138 WP_014470646.1 hypothetical protein -
  M8X21_RS12160 (M8X21_12160) - 2505213..2506331 (+) 1119 WP_029973892.1 NADH-dependent flavin oxidoreductase -
  M8X21_RS12165 (M8X21_12165) - 2506457..2506669 (+) 213 WP_029973891.1 spore germination protein -
  M8X21_RS12170 (M8X21_12170) - 2506795..2507007 (+) 213 WP_014418351.1 spore germination protein -
  M8X21_RS12175 (M8X21_12175) alr 2507182..2508363 (+) 1182 WP_021494302.1 alanine racemase -
  M8X21_RS12180 (M8X21_12180) spo0A 2508511..2509311 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  M8X21_RS12185 (M8X21_12185) spoIVB 2509592..2510872 (-) 1281 WP_029973889.1 SpoIVB peptidase -
  M8X21_RS12190 (M8X21_12190) recN 2511036..2512766 (-) 1731 WP_007408359.1 DNA repair protein RecN Machinery gene
  M8X21_RS12195 (M8X21_12195) argR 2512798..2513247 (-) 450 WP_003153173.1 transcriptional regulator ArgR -
  M8X21_RS12200 (M8X21_12200) - 2513344..2514189 (-) 846 WP_029973888.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=689630 M8X21_RS12180 WP_003153177.1 2508511..2509311(-) (spo0A) [Bacillus velezensis strain HMB26553]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=689630 M8X21_RS12180 WP_003153177.1 2508511..2509311(-) (spo0A) [Bacillus velezensis strain HMB26553]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCTGATGTACTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGATGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCCGCGCAAAGCAGCATTATCCGCAGCCAGCCGGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAACCAACCAATTCAGAATTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97