Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   M8X21_RS06325 Genome accession   NZ_CP097467
Coordinates   1204455..1206284 (+) Length   609 a.a.
NCBI ID   WP_003155028.1    Uniprot ID   -
Organism   Bacillus velezensis strain HMB26553     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1199455..1211284
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8X21_RS06300 (M8X21_06300) - 1200118..1200696 (+) 579 WP_007409110.1 GNAT family N-acetyltransferase -
  M8X21_RS06305 (M8X21_06305) spx 1200875..1201270 (+) 396 WP_003155034.1 transcriptional regulator Spx -
  M8X21_RS06310 (M8X21_06310) - 1201328..1201984 (-) 657 WP_014417421.1 TerC family protein -
  M8X21_RS06315 (M8X21_06315) mecA 1202260..1202916 (+) 657 WP_003155032.1 adaptor protein MecA Regulator
  M8X21_RS06320 (M8X21_06320) - 1203067..1204227 (+) 1161 WP_029973144.1 competence protein CoiA family protein -
  M8X21_RS06325 (M8X21_06325) pepF 1204455..1206284 (+) 1830 WP_003155028.1 oligoendopeptidase F Regulator
  M8X21_RS06330 (M8X21_06330) - 1206322..1206489 (-) 168 WP_003155026.1 hypothetical protein -
  M8X21_RS06335 (M8X21_06335) spxH 1206776..1207678 (-) 903 WP_003155024.1 protease adaptor protein SpxH -
  M8X21_RS06340 (M8X21_06340) - 1207675..1208073 (-) 399 WP_003155023.1 thiol management oxidoreductase -
  M8X21_RS06345 (M8X21_06345) - 1208302..1208988 (-) 687 WP_022553553.1 lytic transglycosylase domain-containing protein -
  M8X21_RS06350 (M8X21_06350) - 1208993..1209565 (-) 573 WP_029973141.1 CYTH domain-containing protein -
  M8X21_RS06355 (M8X21_06355) - 1209690..1210055 (+) 366 WP_012117290.1 hypothetical protein -
  M8X21_RS06360 (M8X21_06360) yjbM 1210083..1210718 (+) 636 WP_003155019.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 69799.43 Da        Isoelectric Point: 5.2024

>NTDB_id=689618 M8X21_RS06325 WP_003155028.1 1204455..1206284(+) (pepF) [Bacillus velezensis strain HMB26553]
MTEENKAAQLPDRSEVKAEHTWRLEDIFPSDEAWNKEFQAVKELIPNLSSFKGTLAHSADNLYAALTAQDEVTNRLGKLY
AYAHMRSDQDTGNSFYQGLNDKAASLYSQAASATAYIEPEILAIQEDKLHQFILEKEELKLYSHAIEEITKQRPHVLSEQ
EEALLAEASEALSSPSNTFSVLNNADITFPSIKDENGKETQITHGNFINFLESSNREVRKNAFEAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLIKTINKHLPLLHRYIDLRKKVLGLDEVHIYDLYTPLVKDSGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRSGAYSSGTYGTNPYILMNWHDNVNNLFTLVHEFGHS
VHSYYTRKYQPYPYGNYSIFVAEVASTTNEALLGEYMLNSLKDEKQRLYLLNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLNELYYDLNKKYFGDNMVIDKEISLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVERYTEFL
KAGSSEYPIDILKKAGVDMTSPEPIEAACKMFEEKLNEMEELLSKVNPS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=689618 M8X21_RS06325 WP_003155028.1 1204455..1206284(+) (pepF) [Bacillus velezensis strain HMB26553]
ATGACTGAGGAAAATAAAGCAGCACAGCTGCCTGACAGAAGTGAAGTAAAGGCAGAGCATACATGGAGACTAGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCAGTTAAAGAATTAATCCCGAATTTATCTTCTTTTAAAGGAA
CGCTTGCTCATTCGGCTGACAATTTATACGCGGCGCTTACGGCACAGGATGAAGTGACGAACCGCCTTGGCAAACTTTAT
GCATACGCGCATATGCGCTCAGACCAGGATACGGGAAATTCTTTCTACCAAGGGCTGAACGACAAAGCCGCAAGCCTGTA
TTCCCAGGCTGCCAGCGCGACTGCTTACATTGAACCGGAAATTCTGGCGATTCAGGAGGACAAGCTGCACCAGTTCATTC
TGGAAAAAGAAGAGCTTAAGCTTTACTCCCATGCAATTGAAGAAATTACAAAACAGCGCCCGCACGTGCTGAGCGAACAA
GAAGAGGCCCTGCTTGCGGAAGCCTCAGAAGCGCTGTCTTCGCCTTCCAATACATTCAGCGTTTTAAATAACGCCGATAT
TACATTTCCGTCGATTAAAGATGAAAACGGCAAGGAAACTCAAATCACACACGGCAACTTTATTAATTTTCTGGAAAGCA
GCAATCGTGAAGTCCGCAAAAACGCATTTGAAGCTGTATATAAAACATACGGACAGTATAAAAACACGATGGCGACTACA
CTCAGCGGCACTGTGAAAAAAGATAATTTTTACGCCCGTGTGAAAAAATATAAATCAGCACGGGAAGCGGCTTTATCCAA
TAACAGCATCCCTGAAGAAGTTTACGATAACCTGATTAAAACGATAAATAAACATCTTCCGTTATTACACCGTTACATTG
ACCTGCGGAAAAAGGTGCTCGGCCTTGATGAAGTCCATATTTATGACCTGTACACACCGCTCGTAAAAGATTCGGGAATG
AAGGTCACATATGAGGAAGCGAAAGATTATATGCTCAAAGGACTTGCTCCGCTGGGCGAGGAATACGCGTCTATTTTAAA
AGAAGGACTTGAAAACCGCTGGGTCGATGTATACGAAAACAAAGGAAAACGCAGCGGTGCTTATTCGTCCGGTACATACG
GCACGAATCCGTACATCTTGATGAACTGGCATGATAATGTCAATAATCTGTTTACGCTCGTTCACGAGTTCGGCCATTCC
GTGCACAGCTACTATACGAGAAAATATCAGCCGTATCCGTACGGTAACTACAGCATTTTTGTCGCTGAAGTCGCTTCAAC
GACTAATGAAGCGCTGCTCGGTGAATATATGCTGAACAGCTTAAAGGATGAAAAACAGCGTCTGTACCTGCTCAACCACA
TGCTTGAAGGATTCAGAGGCACAGTGTTCAGACAGACGATGTTCGCTGAATTTGAGCATCTGATTCATACGAAAGCGCAG
GAAGGCGAGCCGCTGACGCCTGAACTGCTGAACGAACTTTATTATGATCTGAATAAAAAGTATTTCGGTGACAACATGGT
GATTGATAAAGAAATCAGTCTGGAATGGTCAAGAATTCCGCATTTCTATTACAATTATTATGTGTATCAATATGCGACAG
GCTACAGTGCGGCCCAAGCGCTGAGCAGCCAGATCTTAAAAGAAGGCAAGCCTGCGGTGGAGCGCTATACCGAATTCTTA
AAAGCGGGAAGCTCGGAATATCCGATCGACATACTGAAAAAAGCGGGAGTCGATATGACATCGCCTGAACCGATCGAGGC
GGCCTGCAAAATGTTTGAAGAGAAGCTGAACGAAATGGAAGAGCTTCTTTCGAAAGTGAACCCTTCGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(211-590)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50

97.537

0.488