Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M5524_RS06595 Genome accession   NZ_CP097466
Coordinates   1524990..1526051 (-) Length   353 a.a.
NCBI ID   WP_373990653.1    Uniprot ID   -
Organism   Duganella sp. BuS-21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1519990..1531051
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5524_RS06575 (M5524_06550) - 1521305..1521808 (-) 504 WP_373990649.1 hypothetical protein -
  M5524_RS06580 (M5524_06555) rpoH 1522278..1523165 (-) 888 WP_373990650.1 RNA polymerase sigma factor RpoH -
  M5524_RS06585 (M5524_06560) ftsX 1523406..1524326 (-) 921 WP_373990651.1 permease-like cell division protein FtsX -
  M5524_RS06590 (M5524_06565) ftsE 1524323..1524979 (-) 657 WP_373990652.1 cell division ATP-binding protein FtsE -
  M5524_RS06595 (M5524_06570) pilA 1524990..1526051 (-) 1062 WP_373990653.1 signal recognition particle-docking protein FtsY Machinery gene
  M5524_RS06600 (M5524_06575) - 1526175..1527125 (-) 951 WP_373990654.1 MerR family transcriptional regulator -
  M5524_RS06605 (M5524_06580) - 1527398..1527931 (+) 534 WP_373990655.1 chalcone isomerase family protein -
  M5524_RS06610 (M5524_06585) - 1527928..1529235 (+) 1308 WP_373990656.1 MFS transporter -
  M5524_RS06615 (M5524_06590) - 1529239..1529790 (+) 552 WP_373990657.1 DUF3833 domain-containing protein -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 37401.59 Da        Isoelectric Point: 5.7363

>NTDB_id=689579 M5524_RS06595 WP_373990653.1 1524990..1526051(-) (pilA) [Duganella sp. BuS-21]
MFSFFKKKPVAPEAPAAPVVAPAQVAPPAPVTPAVPDLPDLLDLPDLLPIEEPAQKQSWMMRLKAGLSKTSSNLSVLFVG
AKIDDELYEELESALLMADAGVDATTYLLDALKRKVKDDKLLDAAAVKAALKQLLLELLSPLERPFELGRHTPTVMMIAG
VNGAGKTTTIGKLAKHMQTHGQSVLLAAGDTFRAAAREQLMVWGQRNNISVVSQASGDPAAVSFDAVQSGKAKGMDVVMI
DTAGRLPTQLHLMEELKKIQRVLGKGLEGAPHETLLVIDGNTGQNALAQVKAFDDALTLTGLVITKLDGTAKGGVLAAIA
RARPVPVYFIGVGEKIEDLQPFVAAEFVEALLG

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=689579 M5524_RS06595 WP_373990653.1 1524990..1526051(-) (pilA) [Duganella sp. BuS-21]
ATGTTCAGCTTCTTCAAGAAAAAACCTGTCGCTCCCGAAGCGCCTGCGGCGCCTGTCGTCGCCCCTGCCCAAGTCGCCCC
CCCCGCCCCCGTCACGCCCGCTGTGCCCGACTTGCCCGACTTGCTCGACTTGCCCGACCTGCTGCCGATCGAAGAGCCGG
CGCAGAAGCAATCGTGGATGATGCGCCTGAAGGCCGGCCTGTCCAAGACCTCGTCCAACCTGTCGGTGCTGTTCGTCGGC
GCCAAGATCGACGACGAGCTGTACGAGGAGCTGGAATCGGCGCTGTTGATGGCCGACGCCGGCGTCGACGCCACCACCTA
CCTGCTCGACGCCCTCAAGCGCAAGGTCAAGGACGACAAACTGCTGGACGCCGCCGCCGTCAAGGCCGCGCTGAAGCAGC
TACTGCTGGAACTGCTATCGCCGCTGGAGCGCCCGTTCGAACTGGGCCGCCACACGCCAACCGTGATGATGATCGCTGGC
GTCAACGGCGCCGGCAAGACCACCACCATCGGCAAGCTGGCCAAGCATATGCAAACCCACGGCCAATCGGTGCTGCTGGC
CGCCGGCGACACCTTCCGCGCCGCCGCCCGCGAACAGCTGATGGTCTGGGGCCAGCGCAACAACATCAGCGTGGTCTCGC
AAGCTTCGGGCGACCCGGCCGCCGTGTCGTTTGACGCCGTCCAGTCCGGCAAGGCCAAAGGCATGGACGTGGTCATGATC
GACACCGCCGGCCGCCTGCCGACCCAGCTGCACCTGATGGAAGAGCTGAAAAAAATCCAGCGCGTGCTCGGCAAGGGCCT
GGAGGGCGCACCGCACGAAACGCTGCTGGTGATCGACGGCAATACCGGGCAAAATGCGCTGGCGCAGGTGAAAGCCTTCG
ACGACGCTCTCACGCTGACCGGCCTGGTGATCACCAAGCTGGACGGCACGGCCAAGGGCGGCGTGCTGGCCGCCATCGCG
CGCGCGCGTCCGGTGCCGGTGTATTTCATCGGCGTCGGTGAAAAAATTGAAGACCTGCAGCCCTTCGTGGCTGCGGAGTT
TGTCGAAGCACTGCTAGGCTAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(79-133)

SRP54-type protein, GTPase domain (SRP54)

(153-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

59.406

85.836

0.51