Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   M5524_RS04170 Genome accession   NZ_CP097466
Coordinates   974358..975830 (+) Length   490 a.a.
NCBI ID   WP_373990208.1    Uniprot ID   -
Organism   Duganella sp. BuS-21     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 969358..980830
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5524_RS04145 (M5524_04140) - 970865..971854 (+) 990 WP_373990203.1 SIS domain-containing protein -
  M5524_RS04150 (M5524_04145) - 971854..972390 (+) 537 WP_373990204.1 KdsC family phosphatase -
  M5524_RS04155 (M5524_04150) lptC 972402..972995 (+) 594 WP_373990205.1 LPS export ABC transporter periplasmic protein LptC -
  M5524_RS04160 (M5524_04155) lptA 973013..973597 (+) 585 WP_373990206.1 lipopolysaccharide transport periplasmic protein LptA -
  M5524_RS04165 (M5524_04160) lptB 973599..974351 (+) 753 WP_373990207.1 LPS export ABC transporter ATP-binding protein -
  M5524_RS04170 (M5524_04165) rpoN/rpoN1 974358..975830 (+) 1473 WP_373990208.1 RNA polymerase factor sigma-54 Machinery gene
  M5524_RS04175 (M5524_04170) hpf 975908..976267 (+) 360 WP_373990209.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  M5524_RS04180 (M5524_04175) - 976358..977275 (+) 918 WP_373990210.1 alginate lyase family protein -
  M5524_RS04185 (M5524_04180) - 977286..978365 (+) 1080 WP_373990211.1 enoyl-CoA hydratase/isomerase family protein -
  M5524_RS04190 (M5524_04185) - 978382..978594 (-) 213 WP_373990212.1 DNA-binding protein -
  M5524_RS04195 (M5524_04190) - 978587..978892 (-) 306 WP_373990213.1 type II toxin-antitoxin system RelE/ParE family toxin -
  M5524_RS04200 (M5524_04195) rapZ 978942..979802 (-) 861 WP_373990214.1 RNase adapter RapZ -

Sequence

Protein


Download         Length: 490 a.a.        Molecular weight: 54696.39 Da        Isoelectric Point: 5.0435

>NTDB_id=689573 M5524_RS04170 WP_373990208.1 974358..975830(+) (rpoN/rpoN1) [Duganella sp. BuS-21]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQLLTDNPLLERLDDPLDHSLRLLSDGAINQQAPVGEAPPEGP
PNEAPAVEADAYEGPAADTESTTAEADMDWGDAGRSKNSDDEESRPQLEAHHCTLREHLMEQMRVTVLEMRDRALVELII
DALDENGYLEEALEEIHARLPEELEVEIEELHTALSLLQSFDPMGVGARNASECLALQIKRLPGVPMVTRRMALVIVENH
LTWFAQRDFNKLKKTLLCDDEDLREAQAVIRQCNPHPGAAFASDVSDYVVPDVIVKKSRNGWQVSLNNDVMPRLRVNAMY
ANLLKQGKGEGAMGAQLQEAKWLIKNMRQRFDTILRVAQAIVERQKNFFSHGAVAMRPLVLREIADTLGLHESTISRVTT
QKYMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQLTGAEDPKNPLSDSKIADMLGEQGMVIARRTVAKYREALK
IPPVSLRKSL

Nucleotide


Download         Length: 1473 bp        

>NTDB_id=689573 M5524_RS04170 WP_373990208.1 974358..975830(+) (rpoN/rpoN1) [Duganella sp. BuS-21]
ATGAAACAGTCTTTGCAACTGCGCACGTCGCAGCACTTAGCGTTGACGCCACAGTTGCAGCAGTCGATCCGGCTGCTGCA
ACTGTCGACGCTGGAGCTGCACCAGGAACTCGAACAGCTGCTGACCGATAACCCGCTGCTGGAACGTCTCGACGATCCGC
TCGACCACTCGCTGCGCCTGCTGTCCGACGGCGCCATCAACCAGCAGGCGCCGGTCGGCGAAGCGCCGCCCGAAGGTCCG
CCCAACGAAGCACCTGCCGTCGAGGCCGACGCCTACGAGGGCCCGGCCGCCGACACCGAAAGCACCACCGCCGAAGCCGA
CATGGACTGGGGCGACGCCGGCCGCAGCAAGAACAGCGACGACGAGGAATCGCGTCCGCAACTCGAAGCCCATCACTGCA
CGCTGCGTGAGCACCTGATGGAACAGATGCGCGTGACGGTGCTCGAAATGCGCGACCGCGCGCTGGTCGAACTGATCATC
GACGCGCTCGACGAGAACGGCTACCTGGAGGAGGCGCTGGAAGAAATCCACGCGCGCCTGCCGGAAGAACTGGAGGTGGA
GATCGAGGAACTGCATACGGCCTTGAGCCTGCTGCAAAGCTTCGATCCGATGGGCGTGGGCGCGCGCAACGCGTCCGAAT
GCCTGGCGCTGCAAATCAAGCGCTTGCCGGGCGTGCCGATGGTGACGCGGCGCATGGCGCTGGTCATCGTCGAGAACCAC
CTGACCTGGTTCGCGCAGCGCGATTTCAACAAGCTGAAAAAGACCCTGCTGTGCGACGACGAAGACTTGCGTGAAGCGCA
GGCAGTGATCCGCCAGTGCAATCCGCATCCGGGCGCGGCCTTCGCCTCCGACGTGTCGGACTATGTGGTGCCGGACGTGA
TCGTGAAGAAATCGCGCAACGGCTGGCAGGTCAGCTTGAACAACGACGTCATGCCGCGCCTGCGCGTGAACGCCATGTAC
GCCAACCTGCTCAAGCAGGGTAAAGGCGAGGGCGCCATGGGCGCGCAATTGCAGGAGGCCAAATGGCTGATCAAGAATAT
GCGCCAGCGCTTCGATACGATCCTGCGGGTGGCACAAGCGATAGTAGAACGACAAAAGAACTTTTTCTCGCATGGCGCGG
TTGCCATGCGGCCCCTTGTGCTTCGCGAAATTGCTGATACACTGGGTTTACACGAGAGTACTATTTCTCGGGTAACAACT
CAAAAATATATGCTGACCCCGCATGGCATGTTTGAGTTGAAATATTTCTTTGGTAGCCATGTCGCCACCGAAGCGGGGGG
TGAAGCAAGTTCGACGGCGATACGGGCATTGATCGTGCAACTGACAGGAGCAGAAGACCCGAAAAATCCTTTATCCGACA
GTAAGATTGCGGACATGCTGGGAGAGCAAGGCATGGTGATTGCGCGGCGAACTGTTGCCAAATATCGCGAAGCGCTCAAA
ATCCCACCAGTCAGCCTCCGTAAATCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(335-488)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

60.516

100

0.622