Detailed information NTG0689352

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M8G39_RS02085
• Genome accession: NZ_CP097383
• Coordinates: 445434..446582 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain L00-a
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 440434..451582

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M8G39_RS02060 (M8G39_02060)	-	441585..441881 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
M8G39_RS02065 (M8G39_02065)	-	441878..442471 (-)	594	WP_003084544.1	YggT family protein	-
M8G39_RS02070 (M8G39_02070)	proC	442482..443303 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
M8G39_RS02075 (M8G39_02075)	-	443315..444007 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
M8G39_RS02080 (M8G39_02080)	pilT	444222..445256 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
M8G39_RS02085 (M8G39_02085)	pilU	445434..446582 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
M8G39_RS02090 (M8G39_02090)	czcD	446590..447489 (-)	900	WP_023913256.1	CDF family zinc transporter CzcD	-
M8G39_RS02095 (M8G39_02095)	-	447644..448048 (+)	405	WP_003084560.1	NINE protein	-
M8G39_RS02100 (M8G39_02100)	-	448291..449664 (+)	1374	WP_023098360.1	pyridoxal-phosphate dependent enzyme	-
M8G39_RS02105 (M8G39_02105)	-	449661..450845 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=689352 M8G39_RS02085 WP_003110016.1 445434..446582(+) (pilU) [Pseudomonas aeruginosa strain L00-a]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=689352 M8G39_RS02085 WP_003110016.1 445434..446582(+) (pilU) [Pseudomonas aeruginosa strain L00-a]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382