Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   M5E03_RS01985 Genome accession   NZ_CP097374
Coordinates   369136..369564 (-) Length   142 a.a.
NCBI ID   WP_024424305.1    Uniprot ID   A0A5C0WGQ8
Organism   Bacillus safensis strain AHB11     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 364136..374564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5E03_RS01955 (M5E03_01955) - 364296..364625 (+) 330 WP_317222586.1 YckD family protein -
  M5E03_RS01960 (M5E03_01960) - 364671..365435 (-) 765 WP_034624002.1 MBL fold metallo-hydrolase -
  M5E03_RS01965 (M5E03_01965) - 365551..365967 (+) 417 WP_025092811.1 Lrp/AsnC family transcriptional regulator -
  M5E03_RS01970 (M5E03_01970) - 365996..366802 (+) 807 WP_252050065.1 aminoglycoside N(3)-acetyltransferase -
  M5E03_RS01975 (M5E03_01975) - 366804..367190 (-) 387 WP_034283302.1 competence protein ComJ -
  M5E03_RS01980 (M5E03_01980) - 367311..369008 (-) 1698 WP_034283304.1 methyl-accepting chemotaxis protein -
  M5E03_RS01985 (M5E03_01985) nucA/comI 369136..369564 (-) 429 WP_024424305.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  M5E03_RS01990 (M5E03_01990) - 369932..372001 (+) 2070 WP_252050066.1 hydantoinase/oxoprolinase family protein -
  M5E03_RS01995 (M5E03_01995) - 371991..373979 (+) 1989 WP_252050067.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15971.96 Da        Isoelectric Point: 6.2215

>NTDB_id=689236 M5E03_RS01985 WP_024424305.1 369136..369564(-) (nucA/comI) [Bacillus safensis strain AHB11]
MKLLKMILLLLLIVIGVATGYIQLEQGKQETANSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSICTIDRKHSDEQRDRS
LHGVPTKRGYDRDEWPMAMCKEGGTGASVKYVRPSDNRGAGSWVSHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=689236 M5E03_RS01985 WP_024424305.1 369136..369564(-) (nucA/comI) [Bacillus safensis strain AHB11]
ATGAAACTTCTTAAAATGATTCTCTTGCTCTTATTGATTGTTATTGGTGTAGCAACAGGCTATATCCAGCTGGAGCAAGG
GAAACAAGAGACAGCGAATTCATCTTATGACAAAACGATTCATTTTCCATCAGACCGCTATCCCGAAACAGCCAAGCACA
TAGAAGAAGCCATTGATGAAGGTCATTCGTCCATTTGTACAATTGACCGTAAACATAGTGATGAGCAGAGGGATCGATCA
TTACATGGCGTTCCAACAAAGCGCGGGTATGATCGTGACGAATGGCCAATGGCGATGTGCAAAGAAGGAGGCACAGGAGC
TTCTGTCAAATATGTACGCCCTTCTGATAATAGAGGCGCAGGCTCGTGGGTAAGTCATCAATTATCGGATGATCCAGACG
GCACAAGAATCCAATTTATCATCGATTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WGQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.667

80.282

0.535