Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   M5E02_RS08705 Genome accession   NZ_CP097373
Coordinates   1724048..1724428 (+) Length   126 a.a.
NCBI ID   WP_251215771.1    Uniprot ID   -
Organism   Bacillus safensis strain AHB2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1719048..1729428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5E02_RS08695 (M5E02_08695) - 1719545..1722688 (+) 3144 WP_252058132.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  M5E02_RS08700 (M5E02_08700) - 1722881..1723789 (+) 909 WP_048240225.1 trypsin-like serine protease -
  M5E02_RS08705 (M5E02_08705) nucA/comI 1724048..1724428 (+) 381 WP_251215771.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  M5E02_RS08710 (M5E02_08710) - 1724575..1725423 (+) 849 WP_252058133.1 STAS domain-containing protein -
  M5E02_RS08715 (M5E02_08715) - 1725457..1725999 (-) 543 WP_252058134.1 IseA DL-endopeptidase inhibitor family protein -
  M5E02_RS08720 (M5E02_08720) - 1726295..1726738 (+) 444 WP_252058135.1 hypothetical protein -
  M5E02_RS08725 (M5E02_08725) lexA 1726784..1727404 (-) 621 WP_034283422.1 transcriptional repressor LexA -
  M5E02_RS08730 (M5E02_08730) yneA 1727565..1727876 (+) 312 WP_024425130.1 cell division suppressor protein YneA -
  M5E02_RS08735 (M5E02_08735) - 1727894..1728541 (+) 648 WP_073979991.1 recombinase family protein -
  M5E02_RS08740 (M5E02_08740) - 1728611..1728841 (+) 231 WP_048240230.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13693.39 Da        Isoelectric Point: 8.5068

>NTDB_id=689201 M5E02_RS08705 WP_251215771.1 1724048..1724428(+) (nucA/comI) [Bacillus safensis strain AHB2]
MGTLLGGLGEKQAAKGADRYDHVVQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISGFPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=689201 M5E02_RS08705 WP_251215771.1 1724048..1724428(+) (nucA/comI) [Bacillus safensis strain AHB2]
ATGGGCACGTTGTTAGGTGGACTTGGAGAGAAACAGGCAGCAAAAGGGGCTGATCGATATGATCATGTCGTTCAATTTCC
AAAGGAACGTTACCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACCATTGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAGTCATTAAAAGGAATTCCGACAAAACCTGGCTTTGACCGGGATGAATGGCCG
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGCGCAAGCGTTCAATATGTCAGTCCATCTGATAATAGGGGAGCAGGCTCATG
GGTCGGCCATCAAATCAGCGGGTTTCCTGATGGAAAAAGAATATTATTCATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

59.13

91.27

0.54