Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   M5E02_RS05170 Genome accession   NZ_CP097373
Coordinates   1028544..1032248 (+) Length   1234 a.a.
NCBI ID   WP_252057803.1    Uniprot ID   -
Organism   Bacillus safensis strain AHB2     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1023544..1037248
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5E02_RS05155 (M5E02_05155) - 1023850..1024260 (+) 411 WP_025093362.1 DoxX family protein -
  M5E02_RS05160 (M5E02_05160) - 1024457..1024903 (-) 447 WP_048238419.1 ferritin-like domain-containing protein -
  M5E02_RS05165 (M5E02_05165) addB 1025057..1028566 (+) 3510 WP_252057802.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  M5E02_RS05170 (M5E02_05170) addA 1028544..1032248 (+) 3705 WP_252057803.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  M5E02_RS05175 (M5E02_05175) sbcD 1032275..1033450 (+) 1176 WP_142944452.1 exonuclease subunit SbcD -
  M5E02_RS05180 (M5E02_05180) - 1033447..1036842 (+) 3396 WP_252057804.1 SMC family ATPase -
  M5E02_RS05185 (M5E02_05185) - 1036862..1037161 (+) 300 WP_039177660.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142187.35 Da        Isoelectric Point: 5.0840

>NTDB_id=689185 M5E02_RS05170 WP_252057803.1 1028544..1032248(+) (addA) [Bacillus safensis strain AHB2]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEDPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELVKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPA
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEQLPFYPYIKEDLSLVFRSCLELLERA
LALSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNINFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGQRFERAKQEKSIVDFSDLEHYCLRILAEQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHAAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKQFKPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPDSKDTTTEMLLVHLDQQEAESDEEREELETVQLEARVIAKK
IRELVEQPFQVYDAKQQLTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFRLLRKWRDFSMNHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPIVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEDWLIPDFERYQSKTYLDFIGPALIRHQAMSSILEETGNVVLSHPSVFTISFTQASDL
LKEDMSLEKKQQDEVVQALMDGLPVEGYGDAGEEVAARLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSLRRAR
TTLYDRPAFMKKKALTAAEQGTAMHTVMQHIPLPSDVPYDDSRIQQLLDSLQQQDLLTNEQVQSIDQEGITAFFSTSIGQ
KLQKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTGGLEAAAPILKKRYE
TQIALYAKAVERLTNRKLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=689185 M5E02_RS05170 WP_252057803.1 1028544..1032248(+) (addA) [Bacillus safensis strain AHB2]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCCT
TGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCAGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGTCCTGAAGATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAGGAATTAGTCAAAAACCCCGGGTCCCTTCATATGAGAAGACAGCTGTCACTCATGAATCGTGCCAATATTTC
TACCCTGCACTCCTTTTGCTTGCAGGTTCTGCGCACCTTTTATTATGAGATTGACCTAGATCCAGGCTTTCGTTTGGCTG
ATCAAACAGAGGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCAGCG
TTCTTTGAACTGGTTGATCGTTACACTTCTGACCGGCATGATTTAGACTTGCAATGGCTTGTGAAGAGAATTTATGAGTT
TTCCAGATCTCACCCTTCACCTGAGCAATGGATGAGGGCATTCCTTTCTCTTTATGATGTAGATGCTCAAACAAAAGTAG
AACAATTACCGTTTTATCCATATATCAAAGAGGATCTTTCTCTCGTTTTTCGAAGCTGTCTGGAACTGCTCGAACGAGCT
CTCGCTCTATCAAAAGAACCGGGCGGCCCAGCACCGAGAGCGGAAAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCAGGCATCAAGATGATTTTGAGAAGCTTTACGAGCTTTTGCCAAACATCAATTTTAAAAGGCTCAAAACATGTAAAG
GGGACGAATATGACCCTGTGCTGTTAGAAAAAGCCACAGATGCACGCAATCAAGCAAAAAAACAATTAGAAAAGCTCAAA
GATGAATACTTTATGCGAAGTCCCGCTCAGCATCTGAAAAGCTTAGCTGAAATGAAGCCAGTGGTCGAAACACTTGTGGA
GCTTGTCATCCAATTTGGACAGCGATTCGAAAGAGCGAAGCAAGAAAAATCAATCGTGGACTTTTCGGATTTAGAGCATT
ATTGCTTACGCATTTTAGCGGAGCAGGACGCGGAAGGAAATTTGATCGAAACAGAAGCGGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATATCAAGATACAAACCTTGTACAAGAAACAATTTTAAAGCTTGTATCTAAAGGGGA
ACATGCTGCTGAGGGCAACCTGTTTATGGTTGGTGATGTAAAGCAGTCCATTTATCGTTTTAGACTGGCTGAACCCATGC
TCTTTTTAAACAAATATAAACAATTTAAACCAGATGGCAAAGAAACAGGGAAGAGAATTGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTGTTAGACAGTACGAACTTTTTGTTCAAACAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGATAGTAAAGATACAACGACAGAAATGCTCCTTGTTCATTTAG
ATCAACAGGAAGCAGAAAGTGACGAAGAGCGAGAAGAACTAGAGACCGTACAACTTGAAGCGAGAGTCATTGCTAAAAAA
ATAAGAGAATTGGTAGAGCAGCCATTTCAAGTGTACGATGCAAAACAGCAATTGACCCGTAACTTGCAGTATCGGGACAT
TGTGATTTTGCTTCGCTCCATGCCGTGGGCCCCGCAAATGATGGAGGAATTGAAGAAGCAGGGAATCCCTGTGTATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCTCTATTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCAGCTGTTTTAAGATCACCAATTGTTCATTTAGACGAAAATGAAATGGCACTGATCCGTACGAGTGA
TAAGAAAGGCACTTATTATGATGCTGTGAAGGCATTTATGAGTGTGACTCATTCAGATCACCCTACATGTAAAAAACTAG
AACGATTTTTCAGGTTGCTGCGCAAGTGGCGTGATTTCTCCATGAATCATTCAGTAGCGGAATTGATTTGGGAAGTATAC
CGCGATACGCAGTATCTAGATTACGTTGGCGGGATGCCTGGAGGTAAGCAGAGACAGGCGAACCTCCGTGCTCTTTATGA
TCGAGCGAAGCAGTATGAAAAAGCGGCCTTTAGAGGGCTGTTCCGTTTTCTTCGATTTATCGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTTCGCATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAATCGTCTTTACCGCAGGACTCGGCAGGAACTTTAATATGATGGACTTAAACCAGTCCTACTTGCTTGA
TAAAGAATTAGGATTTGGAAGCAAATATATTCATCCAGAGCTGAGAATTAGTTATGCAACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGCTATTATCTGAAGAGCTGAGAGTTCTATATGTGGCGCTCACAAGGGCTAAGGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCTGCAACCGGAGAGGACTGGCTGAT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGTCCTGCTCTTATCCGTCACCAAGCGATGTCAT
CTATTTTAGAAGAAACAGGTAATGTCGTTCTGTCGCATCCATCAGTATTCACAATCTCATTTACTCAAGCTTCTGATCTT
CTAAAAGAAGATATGTCACTTGAAAAGAAGCAGCAGGACGAGGTGGTACAAGCATTGATGGACGGTCTGCCTGTTGAAGG
ATATGGTGATGCTGGTGAGGAAGTAGCAGCGAGACTTTCATGGAGATACCCATATTTAGCGGCCTCACAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTCTTCGAAGAGCTCGT
ACAACGTTGTATGACCGTCCGGCTTTTATGAAGAAAAAAGCACTCACTGCTGCAGAGCAAGGCACCGCAATGCATACAGT
CATGCAGCATATTCCGCTTCCTTCTGACGTGCCCTATGACGATTCACGTATACAGCAGCTGCTCGATTCCTTACAGCAGC
AAGACTTGCTAACAAATGAACAAGTCCAGTCAATTGATCAAGAAGGAATTACAGCTTTCTTCTCTACTTCTATTGGTCAA
AAGCTGCAAAAAGCGGACTGGGTGAAACGAGAGGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGCCATAT
TGATGCAGAGGGAGAGCCTGTCCTTATTCAAGGGATGATTGACTGTTTATTTGAAACGGACGGAAAGCTATATTTACTAG
ACTATAAAACCGATAGAGTGCAAGGCAGATATACAGGCGGTCTTGAAGCAGCTGCGCCTATTTTGAAAAAGCGCTATGAA
ACTCAAATAGCGTTATATGCAAAAGCAGTAGAACGTTTAACAAACAGAAAATTAGAGGAGAAAATTCTTTATTTCTTTGA
CGGGAATTTAGAAATTTCTTTATAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.505

100

0.666