Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   M5E02_RS01960 Genome accession   NZ_CP097373
Coordinates   359888..360316 (-) Length   142 a.a.
NCBI ID   WP_048238125.1    Uniprot ID   -
Organism   Bacillus safensis strain AHB2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 354888..365316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5E02_RS01930 (M5E02_01930) - 355050..355379 (+) 330 WP_048238266.1 YckD family protein -
  M5E02_RS01935 (M5E02_01935) - 355423..356187 (-) 765 WP_252057255.1 MBL fold metallo-hydrolase -
  M5E02_RS01940 (M5E02_01940) - 356303..356719 (+) 417 WP_048238129.1 Lrp/AsnC family transcriptional regulator -
  M5E02_RS01945 (M5E02_01945) - 356748..357554 (+) 807 WP_252057256.1 AAC(3) family N-acetyltransferase -
  M5E02_RS01950 (M5E02_01950) - 357556..357942 (-) 387 WP_252057257.1 competence protein ComJ -
  M5E02_RS01955 (M5E02_01955) - 358063..359760 (-) 1698 WP_252057258.1 methyl-accepting chemotaxis protein -
  M5E02_RS01960 (M5E02_01960) nucA/comI 359888..360316 (-) 429 WP_048238125.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  M5E02_RS01965 (M5E02_01965) - 360684..362753 (+) 2070 WP_252057259.1 hydantoinase/oxoprolinase family protein -
  M5E02_RS01970 (M5E02_01970) - 362743..364731 (+) 1989 WP_252057260.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15953.97 Da        Isoelectric Point: 6.4888

>NTDB_id=689177 M5E02_RS01960 WP_048238125.1 359888..360316(-) (nucA/comI) [Bacillus safensis strain AHB2]
MKLLKIILLLLLIVIGVATGYIKLEQGKQETANSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSICTIDRKHSDEQRDRS
LHGVPTKRGYDRDEWPMAMCKEGGTGASVKYVRPSDNRGAGSWVSHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=689177 M5E02_RS01960 WP_048238125.1 359888..360316(-) (nucA/comI) [Bacillus safensis strain AHB2]
ATGAAACTTCTTAAAATCATTCTCTTGCTTTTATTGATTGTCATTGGTGTAGCAACAGGCTATATCAAGCTGGAGCAAGG
GAAACAAGAGACAGCGAATTCATCTTATGACAAAACCATTCACTTTCCATCAGATCGCTATCCCGAAACAGCCAAGCACA
TAGAAGAAGCCATTGATGAAGGTCATTCGTCCATTTGCACAATTGACCGTAAACATAGCGATGAGCAGAGAGATCGATCA
TTACATGGCGTTCCAACAAAGCGCGGTTATGATCGGGACGAATGGCCAATGGCGATGTGTAAAGAAGGGGGCACAGGAGC
TTCTGTCAAATATGTACGCCCTTCAGATAATAGAGGCGCAGGCTCGTGGGTAAGTCATCAATTATCAGATGATCCTGACG
GCACAAGAATTCAATTTATCATTGATTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.667

80.282

0.535