Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CDC37_RS01655 Genome accession   NZ_AP018254
Coordinates   436039..437145 (+) Length   368 a.a.
NCBI ID   WP_096625809.1    Uniprot ID   -
Organism   Calothrix sp. NIES-3974     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 431039..442145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDC37_RS01640 (NIES3974_03370) - 432094..433050 (+) 957 WP_096620186.1 hypothetical protein -
  CDC37_RS01645 (NIES3974_03380) - 433080..434390 (+) 1311 WP_096620187.1 folylpolyglutamate synthase/dihydrofolate synthase family protein -
  CDC37_RS01650 (NIES3974_03390) - 434557..435861 (-) 1305 WP_096620188.1 sugar ABC transporter substrate-binding protein -
  CDC37_RS01655 (NIES3974_03400) pilM 436039..437145 (+) 1107 WP_096625809.1 type IV pilus biogenesis protein PilM Machinery gene
  CDC37_RS01660 (NIES3974_03410) - 437150..437929 (+) 780 WP_096620189.1 PilN domain-containing protein -
  CDC37_RS01665 (NIES3974_03420) - 437926..438708 (+) 783 WP_096620190.1 pilus assembly protein PilO -
  CDC37_RS01670 (NIES3974_03430) - 439056..441341 (+) 2286 WP_096620191.1 type IV pilus secretin family protein -
  CDC37_RS01675 (NIES3974_03440) - 441704..441946 (-) 243 WP_096620192.1 DUF4351 domain-containing protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40488.66 Da        Isoelectric Point: 4.5575

>NTDB_id=68907 CDC37_RS01655 WP_096625809.1 436039..437145(+) (pilM) [Calothrix sp. NIES-3974]
MIKSLNKLFAKSSQTVGIELSPERVNLACLRKQRQGLKLEAISSAPVPEGVFENGQIADPPAMAKIIQQLLTENKIKASH
VISGVPGRESIVRLIPVPAELDDQELREMVLNHEAGLYLPYPREEADVDYQKLGYFVDEDGIEKVQVLLVGTRKDIVDTY
IRTFEQVGLQVKALEINSFALIRTIREQLRQFGPQEAVVLVDIEFDSTEIAIIVNGVPQFSRTVPIGTYQMQTALSRAMS
LPPSRDIDLLLGMTIPATSMDGGKTGQTAVNPGMAAMMRVLGELTDELRRSIDFYLNQSENLEVAQIFLAGSGGGIAQLD
EFFTQRLSLPTTQIDPVSALSLQIDEEKFSPVQRPGLGIVLGLGMREV

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=68907 CDC37_RS01655 WP_096625809.1 436039..437145(+) (pilM) [Calothrix sp. NIES-3974]
GTGATTAAAAGCTTAAATAAATTATTTGCAAAATCAAGTCAAACGGTTGGCATCGAACTTTCACCAGAAAGAGTTAATTT
GGCTTGTCTGCGCAAACAGCGCCAAGGTTTAAAACTAGAAGCAATTTCCTCTGCCCCAGTTCCCGAAGGCGTATTTGAGA
ACGGACAAATTGCCGACCCACCAGCAATGGCAAAAATTATCCAGCAATTGCTAACAGAAAACAAGATCAAAGCCTCCCAT
GTCATCAGTGGGGTTCCGGGACGGGAATCGATTGTGCGTCTGATTCCGGTTCCAGCTGAACTTGATGACCAGGAATTGCG
GGAAATGGTACTCAACCATGAAGCTGGTTTGTATTTACCCTATCCCCGCGAAGAGGCTGATGTAGATTACCAGAAGTTGG
GATACTTTGTCGATGAGGATGGGATTGAAAAGGTACAAGTTCTGTTGGTGGGAACCCGCAAGGACATTGTTGACACCTAT
ATTCGTACCTTTGAACAGGTTGGTTTACAGGTTAAGGCACTGGAAATCAACAGTTTTGCCCTAATTCGGACAATCCGTGA
ACAACTGCGTCAATTCGGACCCCAGGAAGCCGTGGTACTGGTGGATATTGAATTTGATAGTACGGAAATTGCCATTATTG
TCAACGGTGTACCCCAGTTTTCCCGGACAGTGCCAATTGGTACATACCAAATGCAAACAGCCCTATCACGGGCCATGAGT
TTACCTCCATCGCGGGATATCGATTTACTCCTGGGGATGACCATCCCTGCGACATCAATGGATGGGGGGAAAACCGGACA
GACTGCTGTCAACCCTGGTATGGCAGCAATGATGCGGGTTTTAGGTGAACTGACCGATGAATTGCGTCGTTCCATCGATT
TTTATCTCAACCAAAGTGAAAATCTGGAAGTAGCCCAAATTTTCCTCGCTGGTTCTGGTGGGGGAATAGCCCAACTGGAT
GAATTCTTTACCCAACGTTTGAGTTTACCTACCACCCAAATTGACCCGGTGAGTGCCTTGTCCTTGCAAATAGATGAGGA
AAAATTTTCCCCAGTCCAAAGACCGGGTTTAGGAATTGTTCTAGGTTTAGGAATGCGGGAGGTTTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(283-367)

Type IV pilus assembly protein PilM; (PilM_2)

(17-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

67.03

99.728

0.668

Multiple sequence alignment