Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M5D45_RS08960 Genome accession   NZ_CP097330
Coordinates   1931476..1932843 (-) Length   455 a.a.
NCBI ID   WP_250024492.1    Uniprot ID   -
Organism   Cupriavidus campinensis strain G5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1926476..1937843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5D45_RS08940 (M5D45_08940) - 1926871..1927356 (-) 486 WP_149135809.1 disulfide bond formation protein B -
  M5D45_RS08945 (M5D45_08945) oxlT 1927496..1928782 (-) 1287 WP_377731794.1 oxalate/formate MFS antiporter -
  M5D45_RS08950 (M5D45_08950) - 1928895..1930463 (-) 1569 WP_250025359.1 gamma-glutamyltransferase family protein -
  M5D45_RS08955 (M5D45_08955) - 1930679..1931458 (-) 780 WP_144200590.1 GntR family transcriptional regulator -
  M5D45_RS08960 (M5D45_08960) radA 1931476..1932843 (-) 1368 WP_250024492.1 DNA repair protein RadA Machinery gene
  M5D45_RS08965 (M5D45_08965) alr 1932904..1934013 (-) 1110 WP_144200594.1 alanine racemase -
  M5D45_RS08970 (M5D45_08970) lplT 1934499..1935818 (+) 1320 WP_144200596.1 lysophospholipid transporter LplT -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48388.59 Da        Isoelectric Point: 6.9498

>NTDB_id=688624 M5D45_RS08960 WP_250024492.1 1931476..1932843(-) (radA) [Cupriavidus campinensis strain G5]
MAKTKTVYSCTECGGTTPRWAGQCPQCNAWNTLVESVADSGSSAARRFQPLANSAVVRKLSEIDAADVPRFTTGIDEFDR
VLGGGLVSGGVVLIGGDPGIGKSTLLLQTLANLAGQRRVLYVSGEESGAQIALRAQRLGVDAPALGLLPEIQLEKIQATL
EAEQPEVAVIDSIQTLYSEALTSAPGSVAQVRECAAQLTRIAKSTGITIILVGHVTKEGSLAGPRVLEHIVDTVLYFEGD
THSSHRLIRAFKNRFGAVNELGVFAMTERGLRGISNPSALFLSQHEESVAGSCVLVTQEGTRPLLVEVQALVDTANVPNP
RRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLSIHSSMRNKPLPRGLVVFGEVGLAGEI
RPSPRGQERLKEAAKLGFTVAVIPKANAPKQKIEGIEVIAVDRLEQAIDRVRHLD

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=688624 M5D45_RS08960 WP_250024492.1 1931476..1932843(-) (radA) [Cupriavidus campinensis strain G5]
TTGGCCAAGACCAAGACTGTCTATTCCTGCACCGAGTGCGGCGGTACCACGCCGCGCTGGGCCGGCCAGTGCCCGCAGTG
CAACGCCTGGAACACGCTCGTGGAATCCGTGGCGGATTCCGGTTCCAGCGCCGCGCGGCGCTTCCAGCCGCTGGCCAACT
CGGCCGTGGTGCGCAAGCTGTCCGAGATCGATGCGGCCGATGTGCCGCGCTTCACCACCGGCATCGACGAGTTCGACCGC
GTGCTCGGCGGCGGGCTGGTCTCGGGCGGCGTGGTGCTGATCGGCGGCGACCCCGGCATCGGCAAGTCCACGCTGCTGCT
CCAGACGCTGGCCAATCTGGCCGGCCAGCGGCGCGTGCTCTATGTGAGCGGCGAGGAATCCGGCGCCCAGATCGCGCTGC
GTGCCCAGCGGCTGGGCGTGGATGCCCCGGCGCTGGGCCTGCTGCCGGAGATCCAGCTCGAGAAAATCCAGGCCACGCTC
GAGGCGGAGCAGCCCGAAGTGGCCGTGATCGACTCGATCCAGACCCTGTATTCGGAGGCGCTGACCTCGGCGCCCGGCTC
GGTGGCGCAGGTGCGCGAGTGCGCGGCGCAACTGACGCGCATCGCCAAAAGCACCGGCATCACCATCATCCTGGTCGGCC
ATGTGACCAAGGAAGGCAGCCTGGCCGGGCCGCGCGTGCTGGAACATATCGTCGATACGGTGCTGTACTTCGAAGGGGAC
ACCCATTCGTCGCACCGGCTGATCCGCGCGTTCAAGAACCGCTTCGGCGCGGTCAATGAGCTGGGTGTTTTCGCGATGAC
CGAGCGCGGCTTGCGCGGCATCAGCAACCCGTCGGCGCTGTTCCTCTCGCAGCACGAGGAATCGGTGGCGGGTTCGTGCG
TGCTGGTTACGCAGGAGGGCACCCGGCCGCTGCTGGTGGAGGTGCAGGCGCTGGTCGATACCGCCAACGTGCCCAATCCG
CGCCGGCTGGCGGTGGGGCTGGAGCAGAACCGGCTGGCGATGCTGCTGGCGGTGCTGCACCGGCACGCGGGCATTGCGTG
CTTCGATCAGGACGTGTTCCTGAACGCCGTGGGCGGGGTCAAGATCACCGAACCGGCCGCCGATCTGGCGGTATTGCTGT
CGATCCATTCGTCGATGCGCAACAAGCCGCTGCCGCGCGGGCTGGTGGTGTTCGGCGAAGTGGGGCTGGCGGGCGAGATC
CGCCCGAGCCCGCGCGGGCAGGAGCGGCTCAAGGAGGCCGCCAAGCTCGGCTTCACGGTGGCGGTGATCCCCAAGGCCAA
CGCGCCGAAGCAGAAGATCGAGGGCATCGAGGTGATCGCCGTGGACCGCCTCGAACAAGCCATTGACCGCGTGCGGCACC
TGGACTGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(66-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.993

99.56

0.508
  radA Streptococcus pneumoniae Rx1

46.537

100

0.473
  radA Streptococcus pneumoniae D39

46.537

100

0.473
  radA Streptococcus pneumoniae TIGR4

46.537

100

0.473
  radA Streptococcus pneumoniae R6

46.537

100

0.473
  radA Streptococcus mitis SK321

46.421

100

0.47
  radA Streptococcus mitis NCTC 12261

46.32

100

0.47