Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   M5D45_RS01700 Genome accession   NZ_CP097330
Coordinates   369600..371081 (-) Length   493 a.a.
NCBI ID   WP_144198894.1    Uniprot ID   A0AAE9HZI7
Organism   Cupriavidus campinensis strain G5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 364600..376081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5D45_RS01670 (M5D45_01670) - 365125..365787 (-) 663 WP_144198901.1 LON peptidase substrate-binding domain-containing protein -
  M5D45_RS01675 (M5D45_01675) rapZ 365832..366731 (-) 900 WP_144198898.1 RNase adapter RapZ -
  M5D45_RS01680 (M5D45_01680) - 366796..367128 (-) 333 WP_144198896.1 PsiF family protein -
  M5D45_RS01685 (M5D45_01685) hprK 367237..368208 (-) 972 WP_008650955.1 HPr(Ser) kinase/phosphatase -
  M5D45_RS01690 (M5D45_01690) - 368265..368720 (-) 456 WP_092291690.1 PTS sugar transporter subunit IIA -
  M5D45_RS01695 (M5D45_01695) hpf 369196..369549 (-) 354 WP_092291692.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  M5D45_RS01700 (M5D45_01700) rpoN/rpoN1 369600..371081 (-) 1482 WP_144198894.1 RNA polymerase factor sigma-54 Machinery gene
  M5D45_RS01705 (M5D45_01705) lptB 371215..372015 (-) 801 WP_144198891.1 LPS export ABC transporter ATP-binding protein -
  M5D45_RS01710 (M5D45_01710) lptA 372055..372696 (-) 642 WP_144198889.1 lipopolysaccharide transport periplasmic protein LptA -
  M5D45_RS01715 (M5D45_01715) lptC 372760..373395 (-) 636 WP_144198887.1 LPS export ABC transporter periplasmic protein LptC -
  M5D45_RS01720 (M5D45_01720) - 373408..374391 (-) 984 WP_144198885.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54994.29 Da        Isoelectric Point: 5.1104

>NTDB_id=688616 M5D45_RS01700 WP_144198894.1 369600..371081(-) (rpoN/rpoN1) [Cupriavidus campinensis strain G5]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVSADGSVNLQSAPAPASSEPQ
PNGDARSDGASSGEDDNYNDNGSEDYGSDWSLDDFARRPQGDEDEKTPMQLRDAEPTLREHLMEQLAPLKISVRDKGLAI
FLVESLDDDGYLSASLEEICTELPEELEFEIDEVHAILTLLQSFDPPGVGARNAAECLSLQLRRLKHPHRDLALTIVNHH
LELLAVRDYTRLKKALQVDEIALKAAHELIRSLAPYPGHAFNRPEADFVVPDVFVRKSGSGWIAALNPDVMPRLRINDMY
AQILRGAKGESGTAGLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKNFFTHGEIAMRPLVLREIADTLGLHESTISR
VTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRNPLSDSRIAELLGEQGFVVARRTVAKYRE
ALKIPAVNLRKTL

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=688616 M5D45_RS01700 WP_144198894.1 369600..371081(-) (rpoN/rpoN1) [Cupriavidus campinensis strain G5]
ATGAAACCGTCGCTTCAGCTTCGTCTCTCGCAACATCTGGCATTGACCCCACAGCTGCAACAGTCGATTCGATTGTTGCA
GCTCTCGACGCTTGAACTCCAGCAGGAAGTGGAACAGGCGCTCACCGAAAACCCCCTGCTCGAACGCGAAAACGACTGGA
TCGAAAGCCCGCTGCGGGTGTCCGCCGATGGCTCGGTCAACCTCCAGAGCGCCCCGGCGCCGGCCTCCTCCGAGCCGCAG
CCCAACGGCGACGCGCGCAGCGACGGGGCATCCAGCGGCGAGGACGACAACTACAACGACAACGGCAGCGAGGACTACGG
CAGCGACTGGAGCCTCGACGACTTTGCCCGCCGCCCGCAGGGCGACGAGGACGAAAAGACGCCGATGCAGCTGCGCGACG
CCGAACCCACGCTGCGCGAGCACCTGATGGAGCAGCTGGCCCCGCTGAAGATCTCGGTGCGCGACAAGGGCCTCGCCATC
TTTCTGGTCGAGTCGCTCGACGATGACGGCTACCTGAGCGCCTCGCTCGAGGAAATCTGCACCGAACTGCCCGAGGAACT
CGAATTCGAGATCGACGAAGTCCACGCGATCCTGACCCTGCTGCAGAGCTTCGACCCGCCCGGCGTGGGCGCGCGCAATG
CCGCCGAGTGCCTCAGCCTGCAATTGCGGCGCCTGAAGCACCCGCACCGCGACCTGGCGCTGACCATCGTCAACCATCAC
CTGGAATTGTTGGCCGTGCGCGACTACACACGGCTAAAGAAAGCGCTCCAGGTGGACGAAATAGCACTGAAGGCGGCTCA
CGAACTGATTCGCTCGCTGGCGCCGTATCCGGGGCATGCGTTCAACCGTCCCGAGGCCGACTTCGTGGTGCCCGACGTGT
TCGTGCGCAAGAGCGGCAGCGGGTGGATCGCGGCGCTCAATCCGGACGTCATGCCACGGCTGCGCATCAACGACATGTAT
GCGCAGATTCTGCGCGGCGCGAAGGGCGAATCGGGGACGGCGGGCCTGCAGCAGAAGCTTCAGGAGGCGCGGTGGCTTAT
CAAGAACATCCAGCAACGATTCGACACGATCCTGCGTGTTTCTCAGGCCATTGTCGAACGTCAAAAGAACTTTTTCACGC
ATGGCGAAATCGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTCGGTTTACATGAGTCGACCATTTCCCGG
GTCACCACGAATAAATACATGGCCACGCCCATGGGTACTTTCGAGTTGAAGTACTTCTTTGGGAGCCACGTCTCCACGGA
GACTGGCGGCGCGGCCTCTTCCACGGCAATCCGCGCGCTGATCAAGCAACTGATAGGAGCCGAAGACCCCAGGAATCCCC
TTTCCGACAGCCGGATCGCCGAACTGCTGGGTGAGCAAGGCTTCGTGGTCGCACGCCGCACGGTGGCCAAATACCGCGAA
GCGCTCAAGATCCCCGCAGTGAATCTCCGCAAGACTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(336-491)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(130-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

79.641

100

0.809