Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BSFP_RS08245 Genome accession   NZ_AP018111
Coordinates   1804390..1805895 (+) Length   501 a.a.
NCBI ID   WP_096471806.1    Uniprot ID   A0A1Y1BG49
Organism   Burkholderia stabilis strain FERMP-21014     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1799390..1810895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSFP_RS08220 (BSFP_016260) kdsD 1800633..1801616 (+) 984 WP_096471802.1 arabinose 5-phosphate isomerase KdsD -
  BSFP_RS08225 (BSFP_016270) - 1801616..1802152 (+) 537 WP_047899045.1 KdsC family phosphatase -
  BSFP_RS08230 (BSFP_016280) lptC 1802156..1802770 (+) 615 WP_096471803.1 LPS export ABC transporter periplasmic protein LptC -
  BSFP_RS08235 (BSFP_016290) lptA 1802791..1803456 (+) 666 WP_096471804.1 lipopolysaccharide transport periplasmic protein LptA -
  BSFP_RS08240 (BSFP_016300) lptB 1803453..1804229 (+) 777 WP_096471805.1 LPS export ABC transporter ATP-binding protein -
  BSFP_RS08245 (BSFP_016310) rpoN/rpoN1 1804390..1805895 (+) 1506 WP_096471806.1 RNA polymerase factor sigma-54 Machinery gene
  BSFP_RS08250 (BSFP_016320) hpf 1806053..1806412 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BSFP_RS08255 (BSFP_016330) - 1806610..1807122 (+) 513 WP_034195643.1 PTS sugar transporter subunit IIA -
  BSFP_RS08260 (BSFP_016340) hprK 1807252..1808220 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  BSFP_RS08265 (BSFP_016350) rapZ 1808296..1809204 (+) 909 WP_096471807.1 RNase adapter RapZ -
  BSFP_RS08270 (BSFP_016360) - 1809232..1809867 (+) 636 WP_065499174.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54739.81 Da        Isoelectric Point: 4.8237

>NTDB_id=68861 BSFP_RS08245 WP_096471806.1 1804390..1805895(+) (rpoN/rpoN1) [Burkholderia stabilis strain FERMP-21014]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPGQNTEPAP
ANSGSTSGDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTATLDEVLADLPDELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=68861 BSFP_RS08245 WP_096471806.1 1804390..1805895(+) (rpoN/rpoN1) [Burkholderia stabilis strain FERMP-21014]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACACCCCAGCTACAGCAGTCGATCCGGCTTCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCCGCGGACGGATCGCTGATCGCGCAGACACCTCCCGGCCAGAACACCGAGCCGGCCCCG
GCAAACAGCGGCAGCACGTCGGGCGATCGCGCGGAGCGCGACGAGCCGGCCGGTGCCGACGAATACGACGGTTATGGGTC
CGGCGACGGCAACGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCTCGAGCGGCGCGTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGTTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGGACGAACTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGGGCCCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCACGCGACTTCACGCGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTGAAGCAGCA
GCTTCAGGAAGCACGCTGGCTGATCAAGAACATTCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACTGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCACACGTTTCAACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y1BG49

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.515

100

0.691

Multiple sequence alignment