Detailed information NTG0688595

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: M5L76_RS17680
• Genome accession: NZ_CP097328
• Coordinates: 3581770..3582915 (+)
• Length: 381 a.a.
• NCBI ID: WP_074534220.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain ES2-4
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3576770..3587915

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M5L76_RS17660 (M5L76_17660)	-	3577667..3578095 (-)	429	WP_032869448.1	DUF1934 domain-containing protein	-
M5L76_RS17665 (M5L76_17665)	-	3578249..3578761 (+)	513	Protein_3426	bacteriocin biosynthesis protein AlbG	-
M5L76_RS17670 (M5L76_17670)	-	3578768..3580144 (-)	1377	WP_032875890.1	YncE family protein	-
M5L76_RS17675 (M5L76_17675)	-	3580184..3581539 (-)	1356	WP_032875887.1	YncE family protein	-
M5L76_RS17680 (M5L76_17680)	rapF	3581770..3582915 (+)	1146	WP_074534220.1	Rap family tetratricopeptide repeat protein	Regulator
M5L76_RS17685 (M5L76_17685)	-	3582899..3583018 (+)	120	WP_017418707.1	PhrC/PhrF family phosphatase-inhibitory pheromone	-
M5L76_RS17690 (M5L76_17690)	speB	3583141..3584013 (-)	873	WP_032875884.1	agmatinase	-
M5L76_RS17695 (M5L76_17695)	speE	3584072..3584902 (-)	831	WP_003151052.1	spermidine synthase	-
M5L76_RS17700 (M5L76_17700)	-	3585102..3587174 (+)	2073	WP_032875881.1	transglycosylase domain-containing protein	-
M5L76_RS17705 (M5L76_17705)	-	3587199..3587632 (-)	434	Protein_3434	hypothetical protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45737.21 Da
• Isoelectric Point: 5.0614

>NTDB_id=688595 M5L76_RS17680 WP_074534220.1 3581770..3582915(+) (rapF) [Bacillus velezensis strain ES2-4]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAESKLTYVKDHIEKAEFYFKMSEVYYYMKQTYVS
MDYARQAYLIYKDAEKYNIRLTQCHSLFGANYLDVKQYDHAISHFQKAYTMAELEQQPQLMGRTLFNIALCHNNQNNKVE
AIPHLKEAIAVFEKAQMSHSLPQPYFLLTQIYFKLKNIDNAYDYHKKGISICKEIDDIIYEEKFKFLEYLYMPDPRDTLI
NDCLHFLENKLMYADIEELALDVAKYYYTQEEYKKASVYFLKVEEARQQIEGGVNLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=688595 M5L76_RS17680 WP_074534220.1 3581770..3582915(+) (rapF) [Bacillus velezensis strain ES2-4]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGTCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAAAGCAAACTGACGTACG
TTAAAGATCATATAGAAAAAGCTGAATTTTACTTTAAAATGTCAGAAGTCTATTATTATATGAAACAAACTTATGTATCG
ATGGATTATGCGCGGCAGGCTTATTTAATTTATAAAGATGCAGAAAAATATAATATTAGATTAACCCAGTGCCATTCTTT
ATTTGGGGCTAATTATCTTGATGTAAAGCAATACGATCACGCGATTTCCCACTTTCAAAAGGCATATACGATGGCTGAAT
TGGAGCAGCAGCCGCAATTAATGGGGAGGACACTTTTTAACATCGCTTTATGCCATAATAATCAAAACAATAAAGTCGAG
GCTATTCCCCATTTAAAAGAAGCCATAGCTGTTTTTGAAAAAGCTCAAATGAGTCATTCACTTCCGCAGCCCTACTTCTT
ACTTACACAGATTTATTTTAAGTTAAAAAATATAGATAACGCGTACGATTATCATAAAAAAGGTATCTCAATATGTAAGG
AAATAGATGATATAATTTACGAGGAAAAATTTAAATTTTTAGAATACTTGTATATGCCGGATCCAAGAGATACATTAATA
AACGATTGTCTGCATTTTCTTGAAAATAAATTGATGTATGCTGATATTGAGGAATTAGCCCTTGATGTAGCTAAATATTA
TTACACACAAGAAGAATATAAAAAAGCTTCTGTCTACTTTTTAAAGGTTGAAGAAGCCAGACAGCAGATCGAAGGAGGAG
TGAATTTATATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (5-65)

• Tetratricopeptide repeat (TPR_12) (188-254)

• Tetratricopeptide repeat (TPR_8) (265-293)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	70.079	100	0.701
rapC	Bacillus subtilis subsp. subtilis str. 168	54.211	99.738	0.541