Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   M5J22_RS08825 Genome accession   NZ_CP097326
Coordinates   1822348..1822782 (+) Length   144 a.a.
NCBI ID   WP_095315408.1    Uniprot ID   -
Organism   Bacillus velezensis strain GUCC45     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1817348..1827782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5J22_RS08820 (M5J22_08820) - 1820848..1822059 (-) 1212 WP_057080788.1 cytochrome P450 -
  M5J22_RS08825 (M5J22_08825) nucA/comI 1822348..1822782 (+) 435 WP_095315408.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  M5J22_RS08830 (M5J22_08830) - 1822842..1823597 (+) 756 WP_003154084.1 YoaK family protein -
  M5J22_RS08835 (M5J22_08835) - 1823631..1823993 (-) 363 WP_003154082.1 hypothetical protein -
  M5J22_RS08840 (M5J22_08840) - 1824186..1825514 (-) 1329 WP_032857388.1 S8 family peptidase -
  M5J22_RS08845 (M5J22_08845) - 1825694..1825927 (+) 234 WP_024085328.1 hypothetical protein -
  M5J22_RS08850 (M5J22_08850) - 1826183..1826890 (+) 708 WP_003154076.1 poly-gamma-glutamate hydrolase family protein -
  M5J22_RS08855 (M5J22_08855) - 1826950..1827402 (+) 453 WP_014305039.1 OsmC family protein -
  M5J22_RS08860 (M5J22_08860) - 1827416..1827769 (-) 354 WP_003154073.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15551.50 Da        Isoelectric Point: 6.8606

>NTDB_id=688465 M5J22_RS08825 WP_095315408.1 1822348..1822782(+) (nucA/comI) [Bacillus velezensis strain GUCC45]
MNAFMKWAASLLLVISLQFVLTGAGIHSDSAAHAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=688465 M5J22_RS08825 WP_095315408.1 1822348..1822782(+) (nucA/comI) [Bacillus velezensis strain GUCC45]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGTCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCATGCTGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAACAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTATGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCATCGGATAACCGCGGGGCCGGTTCATGGGTCGGAAATCAAATGAGCGGCTATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56

86.806

0.486