Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M2M59_RS15815 Genome accession   NZ_CP097314
Coordinates   3115212..3116588 (-) Length   458 a.a.
NCBI ID   WP_147197434.1    Uniprot ID   -
Organism   Rummeliibacillus sp. G93     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3110212..3121588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2M59_RS15795 (M2M59_15795) gltX 3111011..3112468 (-) 1458 WP_147197428.1 glutamate--tRNA ligase -
  M2M59_RS15800 (M2M59_15800) ispF 3112546..3113022 (-) 477 WP_066790974.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  M2M59_RS15805 (M2M59_15805) ispD 3113024..3113710 (-) 687 WP_147197430.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  M2M59_RS15810 (M2M59_15810) - 3113785..3114900 (-) 1116 WP_147197432.1 PIN/TRAM domain-containing protein -
  M2M59_RS15815 (M2M59_15815) radA 3115212..3116588 (-) 1377 WP_147197434.1 DNA repair protein RadA Machinery gene
  M2M59_RS15820 (M2M59_15820) - 3116706..3119144 (-) 2439 WP_147197436.1 ATP-dependent Clp protease ATP-binding subunit -
  M2M59_RS15825 (M2M59_15825) - 3119172..3120242 (-) 1071 WP_147197438.1 protein arginine kinase -
  M2M59_RS15830 (M2M59_15830) - 3120232..3120798 (-) 567 WP_066790986.1 UvrB/UvrC motif-containing protein -
  M2M59_RS15835 (M2M59_15835) - 3120788..3121282 (-) 495 WP_066790988.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50286.86 Da        Isoelectric Point: 7.0793

>NTDB_id=688382 M2M59_RS15815 WP_147197434.1 3115212..3116588(-) (radA) [Rummeliibacillus sp. G93]
MAKKKTKFFCNACGYESPKWLGRCPGCGDWNTMVEEVEVVSKGPRGAFQHSSSVTQKATPLIAVETEDAPRIETELNELN
RVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSAILSNKGHRVLYISGEESIRQTKLRAERLGVKSEELYIYSETNLEFLNQ
TIEEVNPKFVIVDSIQTVYHPNVTSAPGSVSQVRECTAEFMRIAKTKNIAIFLVGHVTKEGQIAGPRILEHMVDTVLYFE
GERHHTYRILRSQKNRFGSTNEIAIFEMLQNGLKEVLNPSELFLQERSQGAPGSTVVASMEGTRPILVEVQALVTPSSFN
YPKRMATGVDQNRISLLMAVLEKRMGMLLQAQDAYIKVAGGVKLDEPAIDLAVLVSIVSSFRDTAAKPMDCYIGEVGLTG
EVRRVSRIEQRIQEAAKLGFKRAIVPASNLGGFEYPDTIEVVGVDTVNQALKLSFDKA

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=688382 M2M59_RS15815 WP_147197434.1 3115212..3116588(-) (radA) [Rummeliibacillus sp. G93]
ATGGCCAAAAAGAAGACAAAGTTTTTTTGTAATGCATGTGGATATGAATCTCCAAAATGGCTAGGTAGATGTCCTGGTTG
CGGTGATTGGAATACAATGGTGGAAGAAGTAGAAGTAGTATCCAAGGGGCCAAGAGGGGCTTTTCAGCATTCCTCGTCTG
TAACGCAAAAAGCTACGCCATTAATTGCAGTTGAAACAGAAGATGCACCAAGGATCGAAACGGAACTAAATGAATTGAAT
CGCGTCCTAGGAGGTGGGATTGTCCCAGGCTCACTTGTCCTAATTGGCGGGGATCCGGGGATTGGGAAATCCACGCTTTT
GCTGCAAGTTTCAGCGATTCTTTCGAATAAAGGGCATCGTGTCCTCTATATTTCCGGAGAAGAATCTATTCGCCAAACGA
AGCTAAGAGCTGAAAGACTAGGCGTAAAATCCGAAGAGCTTTACATTTATTCAGAAACGAATCTTGAATTTCTAAATCAA
ACGATTGAAGAAGTGAACCCCAAGTTTGTTATTGTGGACTCCATTCAAACAGTGTACCACCCGAATGTAACAAGCGCACC
GGGAAGTGTCTCACAGGTTAGAGAATGTACTGCTGAATTTATGCGTATCGCGAAAACGAAGAACATTGCAATTTTCTTAG
TTGGACATGTGACGAAGGAAGGCCAAATCGCAGGGCCAAGGATACTTGAACATATGGTGGATACGGTCCTTTACTTTGAA
GGAGAACGCCACCACACTTATCGCATTCTACGGAGCCAAAAAAATAGATTTGGTTCTACTAATGAAATTGCTATTTTTGA
AATGCTCCAAAATGGTTTGAAGGAAGTATTAAACCCTTCAGAATTGTTTCTGCAAGAACGTTCACAGGGTGCCCCGGGCT
CCACTGTTGTGGCCTCTATGGAAGGGACTCGCCCTATCTTAGTCGAAGTACAAGCTCTCGTGACGCCTTCAAGTTTTAAT
TATCCAAAGCGAATGGCAACAGGTGTAGACCAAAACCGAATATCCTTATTGATGGCAGTATTAGAAAAAAGGATGGGCAT
GCTTTTACAGGCACAAGATGCTTATATAAAGGTAGCAGGCGGGGTAAAGCTAGATGAGCCTGCTATTGATTTAGCGGTTC
TTGTAAGCATTGTTTCAAGCTTTAGAGATACAGCTGCTAAACCAATGGATTGTTATATCGGAGAAGTAGGATTAACAGGT
GAAGTGCGAAGAGTGTCACGGATTGAACAAAGAATCCAGGAAGCAGCCAAACTAGGGTTCAAACGCGCGATTGTCCCAGC
ATCCAACTTGGGTGGCTTTGAGTATCCAGATACCATTGAAGTAGTCGGGGTAGATACAGTTAATCAAGCACTAAAATTAA
GTTTTGATAAGGCATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-35)

AAA domain (AAA_25)

(65-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

70.087

100

0.701
  radA Streptococcus pneumoniae Rx1

63.158

99.563

0.629
  radA Streptococcus pneumoniae D39

63.158

99.563

0.629
  radA Streptococcus pneumoniae R6

63.158

99.563

0.629
  radA Streptococcus pneumoniae TIGR4

63.158

99.563

0.629
  radA Streptococcus mitis NCTC 12261

63.158

99.563

0.629
  radA Streptococcus mitis SK321

63.158

99.563

0.629