Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   M5C96_RS23795 Genome accession   NZ_CP097269
Coordinates   5074921..5076507 (-) Length   528 a.a.
NCBI ID   WP_272565716.1    Uniprot ID   A0AAN5B0S1
Organism   Acidovorax sp. GBBC 1281     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5069921..5081507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5C96_RS23775 (M5C96_23770) - 5070326..5070805 (-) 480 WP_272565711.1 putative toxin-antitoxin system toxin component, PIN family -
  M5C96_RS23780 (M5C96_23775) - 5070795..5072066 (-) 1272 WP_272565712.1 THUMP domain-containing protein -
  M5C96_RS23785 (M5C96_23780) - 5072273..5074135 (+) 1863 WP_272565713.1 methyl-accepting chemotaxis protein -
  M5C96_RS23790 (M5C96_23785) - 5074349..5074894 (+) 546 WP_272565715.1 TspO/MBR family protein -
  M5C96_RS23795 (M5C96_23790) rpoN/rpoN1 5074921..5076507 (-) 1587 WP_272565716.1 RNA polymerase factor sigma-54 Machinery gene
  M5C96_RS23800 (M5C96_23795) lptB 5076596..5077381 (-) 786 WP_272565717.1 LPS export ABC transporter ATP-binding protein -
  M5C96_RS23805 (M5C96_23800) lptA 5077381..5078034 (-) 654 WP_272565719.1 lipopolysaccharide transport periplasmic protein LptA -
  M5C96_RS23810 (M5C96_23805) - 5078139..5078798 (-) 660 WP_272565721.1 thiol:disulfide interchange protein DsbA/DsbL -
  M5C96_RS23815 (M5C96_23810) - 5078856..5079503 (-) 648 WP_272565722.1 SPOR domain-containing protein -
  M5C96_RS23820 (M5C96_23815) argS 5079516..5081324 (-) 1809 WP_272565723.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 528 a.a.        Molecular weight: 57515.55 Da        Isoelectric Point: 4.6499

>NTDB_id=688246 M5C96_RS23795 WP_272565716.1 5074921..5076507(-) (rpoN/rpoN1) [Acidovorax sp. GBBC 1281]
MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAAREEFGLPQADAPVSEDDRHTEEAIY
SGAPGESSAAVESSSEGDAASAAEAPGSADAPDWEGDGTVEMAPDDGEWGGDAPARTRTAGEGDEADATELARSHESLTA
YLHRQALGLRLSEVDTAALRFLIESLNDDGYLDEPLPSLAVSLAGEDDTEQVEELVHRFTVALGLLQSLDPVGVGARGLA
ECLTLQLKALREDGEGDPDVVQTALRICQQPLEMLARRDVRRLAQACGGGEERTRAAMALIARLEPRPGRAFAEVERNII
VPDVIVKKTGRGTQQNFSVQLNPDVMPRLRVHDIYAGALRGNRGGEGHQGLQQRLQEARWFIKNIQQRFDTILRVSRAIV
DRQKNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTETGGNASSTAVRALIKQFV
SAESPAKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1587 bp        

>NTDB_id=688246 M5C96_RS23795 WP_272565716.1 5074921..5076507(-) (rpoN/rpoN1) [Acidovorax sp. GBBC 1281]
ATGAAGCCGGGGCTGTCGCTGCGCGTCTCGCAGCATCTGGCACTCACGCCGCAGTTGCAGCAGTCCATCCGGCTGCTGCA
GTTGTCCACCCTGGAACTGTCGCAAGAGGTCGAGCAGATGCTCGACGAAAACCCCTTCCTTGAACGCACCGCCGAAGAGG
CCGCGCGCGAAGAATTCGGCCTCCCGCAGGCCGATGCGCCCGTGAGCGAGGACGATCGCCACACCGAAGAGGCCATCTAT
TCCGGCGCGCCCGGCGAGTCCAGCGCGGCGGTGGAGTCCTCTTCGGAGGGCGATGCCGCGAGCGCCGCCGAGGCGCCGGG
CAGCGCCGACGCCCCCGACTGGGAAGGCGACGGCACCGTCGAGATGGCGCCGGACGACGGCGAATGGGGCGGCGACGCCC
CGGCGCGCACGCGCACGGCCGGCGAGGGCGACGAAGCCGACGCCACCGAACTGGCCCGCAGCCACGAGTCGCTCACCGCC
TACCTGCACCGGCAGGCGTTGGGCCTGCGCCTGTCCGAGGTCGACACCGCCGCTTTGCGTTTTCTCATCGAGTCGCTCAA
CGACGATGGCTACCTGGACGAGCCGCTGCCGTCGCTCGCCGTGAGCCTGGCGGGCGAGGACGACACCGAGCAGGTCGAGG
AGCTGGTGCACCGCTTCACCGTGGCGCTGGGCCTGCTGCAGAGCCTGGACCCGGTGGGCGTGGGCGCGCGCGGACTGGCC
GAGTGCCTGACCCTGCAGCTCAAGGCGCTGCGCGAGGACGGCGAGGGCGACCCCGACGTGGTGCAGACCGCGCTGCGCAT
CTGCCAGCAGCCGCTGGAGATGCTCGCGCGGCGCGACGTGCGCCGCCTGGCGCAGGCCTGCGGTGGCGGCGAGGAGCGCA
CGCGCGCGGCCATGGCGCTGATCGCCCGCCTGGAGCCCCGGCCCGGCCGCGCCTTCGCCGAGGTGGAGCGCAACATCATC
GTGCCCGACGTCATCGTGAAGAAGACCGGGCGCGGCACGCAGCAGAACTTCAGCGTGCAGCTCAACCCCGATGTGATGCC
GCGCCTGCGCGTGCACGACATCTACGCCGGCGCGCTGCGCGGCAACCGTGGCGGCGAGGGCCACCAGGGCCTGCAGCAGC
GGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGTCGCGCGCCATCGTG
GACCGGCAGAAGAACTTCTTCGTGCACGGCGAGCTGGCCATGCGGCCGCTGGTGCTGCGCGACATCGCCGATGAGCTGGG
CCTGCACGAGTCCACCATCAGCCGCGTGACCACGGCCAAGTACATGGCCACGCCGCAGGGCACGTACGAGCTCAAGTATT
TCTTCGGCTCGGGCCTGGGCACCGAGACCGGGGGCAACGCCTCCAGCACGGCGGTGCGGGCGCTCATCAAGCAGTTCGTC
TCGGCCGAGAGCCCGGCCAAGCCGCTGTCGGACAGCCAGATCGCCGAGATGCTGAAGGAGCAGGGCATCGAGTGCGCGCG
CCGCACCGTGGCCAAGTACCGCGAGGCGCTCAAGATCGCGCCGGCCAACCTGCGCAAGGCGCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(149-355)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(370-526)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.03

100

0.53